GSS Antibody - #DF6214

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Product: GSS Antibody
Catalog: DF6214
Description: Rabbit polyclonal antibody to GSS
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 52kDa; 52kD(Calculated).
Uniprot: P48637
RRID: AB_2838180

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(100%), Zebrafish(83%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(92%), Chicken(100%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
GSS Antibody detects endogenous levels of total GSS.
RRID:
AB_2838180
Cite Format: Affinity Biosciences Cat# DF6214, RRID:AB_2838180.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

epididymis secretory sperm binding protein Li 64p; epididymis secretory sperm binding protein Li 88n; Glutathione synthase; Glutathione synthetase; GSH S; GSH synthetase; GSH-S; GSHB_HUMAN; GSHS; GSS; HEL-S-64p; HEL-S-88n; MGC14098; OTTHUMP00000030711;

Immunogens

Immunogen:
Uniprot:

P48637(GSHB_HUMAN) >>Visit HPA database.

Gene(ID):
GSS(2937)
Description:
Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency. [provided by RefSeq, Jul 2008]
Sequence:
MATNWGSLLQDKQQLEELARQAVDRALAEGVLLRTSQEPTSSEVVSYAPFTLFPSLVPSALLEQAYAVQMDFNLLVDAVSQNAAFLEQTLSSTIKQDDFTARLFDIHKQVLKEGIAQTVFLGLNRSDYMFQRSADGSPALKQIEINTISASFGGLASRTPAVHRHVLSVLSKTKEAGKILSNNPSKGLALGIAKAWELYGSPNALVLLIAQEKERNIFDQRAIENELLARNIHVIRRTFEDISEKGSLDQDRRLFVDGQEIAVVYFRDGYMPRQYSLQNWEARLLLERSHAAKCPDIATQLAGTKKVQQELSRPGMLEMLLPGQPEAVARLRATFAGLYSLDVGEEGDQAIAEALAAPSRFVLKPQREGGGNNLYGEEMVQALKQLKDSEERASYILMEKIEPEPFENCLLRPGSPARVVQCISELGIFGVYVRQEKTLVMNKHVGHLLRTKAIEHADGGVAAGVAVLDNPYPV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Chicken
100
Rabbit
100
Dog
92
Xenopus
92
Zebrafish
83
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P48637 As Substrate

Site PTM Type Enzyme
A2 Acetylation
K12 Ubiquitination
K108 Ubiquitination
K112 Ubiquitination
S137 Phosphorylation
K141 Ubiquitination
T147 Phosphorylation
S149 Phosphorylation
K178 Ubiquitination
S181 Phosphorylation
K186 Acetylation
K186 Ubiquitination
Y199 Phosphorylation
K213 Ubiquitination
R230 Methylation
T238 Phosphorylation
K245 Ubiquitination
R252 Methylation
Y270 Phosphorylation
K293 Ubiquitination
K306 Ubiquitination
K364 Ubiquitination
K384 Ubiquitination
K400 Ubiquitination
S415 Phosphorylation
K443 Ubiquitination
K452 Ubiquitination

Research Backgrounds

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the eukaryotic GSH synthase family.

Research Fields

· Cellular Processes > Cell growth and death > Ferroptosis.   (View pathway)

· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.

· Metabolism > Metabolism of other amino acids > Glutathione metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). Reconstructing hepatic metabolic profile and glutathione-mediated metabolic fate of acrylamide. Environmental Pollution, 2023 (PubMed: 37673322) [IF=8.9]
2). Poria cocos polysaccharides improve alcoholic liver disease by interfering with ferroptosis through NRF2 regulation. Aging, 2024 (PubMed: 38507458) [IF=5.2]
3). DL-3-n-butylphthalide improves ventricular function, and prevents ventricular remodeling and arrhythmias in post-MI rats. Naunyn-Schmiedeberg's Archives of Pharmacology, 2018 (PubMed: 29602954) [IF=3.6]

Application: WB    Species: rat    Sample: ventricular

Fig. 6 |NBP upregulated PI3k/Akt/Nrf2 signaling pathway and downstream antioxidant enzymes, including HO-1, GSH, SOD1, and SOD2, analyzed by western blot. Data are expressed as means + SD. *P < 0.05 versus Sham group; #P < 0.05 versus MI group

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