Product: PSMD4 Antibody
Catalog: DF6206
Description: Rabbit polyclonal antibody to PSMD4
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 41kDa, 52kDa; 41kD(Calculated).
Uniprot: P55036
RRID: AB_2838172

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
PSMD4 Antibody detects endogenous levels of total PSMD4.
RRID:
AB_2838172
Cite Format: Affinity Biosciences Cat# DF6206, RRID:AB_2838172.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

26S protease subunit S5a; 26S proteasome non ATPase regulatory subunit 4; 26S proteasome non-ATPase regulatory subunit 4; 26S proteasome regulatory subunit rpn10; 26S proteasome regulatory subunit S5A; AF 1; AF; AF1; Angiocidin; Antisecretory factor 1; ASF; DS5a; MCB 1; MCB1; Multiubiquitin chain binding protein; Multiubiquitin chain-binding protein; OTTHUMP00000059963; Prosome macropain; Proteasome (prosome macropain) 26S subunit non ATPase 4; Proteasome 26S non ATPase subunit 4; Proteasome 26S subunit non ATPase 4; PSMD 4; Psmd4; PSMD4_HUMAN; pUB R5; pUBR5; Rpn 10; Rpn10; RPN10 homolog; S5A; S5a/antisecretory factor protein;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Description:
S5a (PSMD4) is a subunit of the 19S regulatory proteasome complex functioning in ubiquitinated-protein targeting and degradation (1). S5a contains two polyubiquitin binding motifs (UIM) that bind multiubiquitin chains by hydrophobic interaction (2,3). In addition to ubiquitin, the UIM of S5a shows high affinity to a ubiquitin-like domain present in many proteins. S5a binds to these types of proteins directly and mediates their targeting to the proteasome for degradation (4,5).
Sequence:
MVLESTMVCVDNSEYMRNGDFLPTRLQAQQDAVNIVCHSKTRSNPENNVGLITLANDCEVLTTLTPDTGRILSKLHTVQPKGKITFCTGIRVAHLALKHRQGKNHKMRIIAFVGSPVEDNEKDLVKLAKRLKKEKVNVDIINFGEEEVNTEKLTAFVNTLNGKDGTGSHLVTVPPGPSLADALISSPILAGEGGAMLGLGASDFEFGVDPSADPELALALRVSMEEQRQRQEEEARRAAAASAAEAGIATTGTEDSDDALLKMTISQQEFGRTGLPDLSSMTEEEQIAYAMQMSLQGAEFGQAESADIDASSAMDTSEPAKEEDDYDVMQDPEFLQSVLENLPGVDPNNEAIRNAMGSLASQATKDGKKDKKEEDKK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Horse
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P55036 As Substrate

Site PTM Type Enzyme
C9 S-Nitrosylation
C37 S-Nitrosylation
K40 Ubiquitination
K74 Ubiquitination
K81 Ubiquitination
K83 Ubiquitination
C87 S-Nitrosylation
K98 Ubiquitination
S115 Phosphorylation
K122 Acetylation
K122 Ubiquitination
K126 Acetylation
K126 Ubiquitination
K129 Ubiquitination
K133 Ubiquitination
K135 Ubiquitination
K152 Ubiquitination
S242 Phosphorylation
T250 Phosphorylation
T253 Phosphorylation
S256 Phosphorylation
K262 Ubiquitination
S266 Phosphorylation
Y326 Phosphorylation
S358 Phosphorylation
S361 Phosphorylation
K365 Acetylation
K365 Ubiquitination
K368 Acetylation
K369 Ubiquitination

Research Backgrounds

Function:

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMD4 acts as an ubiquitin receptor subunit through ubiquitin-interacting motifs and selects ubiquitin-conjugates for destruction. Displays a preferred selectivity for longer polyubiquitin chains.

Subunit Structure:

Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits, a base containing 6 ATPases and few additional components including PSMD4. Interacts with NUB1. Interacts with SQSTM1. Interacts with UBQLN4. Interacts with UBE3A. Interacts with UBQLN1 (via ubiquitin-like domain). Interacts with DDI2.

Family&Domains:

The 2 UIM motifs are involved in the binding to a multi-ubiquitin chain in a cooperative way.

Belongs to the proteasome subunit S5A family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Proteasome.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

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