MCM2 Antibody - #DF6201

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Product: MCM2 Antibody
Catalog: DF6201
Description: Rabbit polyclonal antibody to MCM2
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Xenopus
Mol.Wt.: 102kDa; 102kD(Calculated).
Uniprot: P49736
RRID: AB_2838167

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Xenopus(86%)
Clonality:
Polyclonal
Specificity:
MCM2 Antibody detects endogenous levels of total MCM2.
RRID:
AB_2838167
Cite Format: Affinity Biosciences Cat# DF6201, RRID:AB_2838167.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BM28; CCNL 1; CCNL1; CDC like 1; cdc19; CDCL 1; CDCL1; Cell devision cycle like 1; Cyclin like 1; D3S3194; DNA replication licensing factor MCM2; KIAA0030; MCM 2; MCM2; MCM2 minichromosome maintenance deficient 2 mitotin; MCM2 minichromosome maintenance deficient 2 mitotin (S. cerevisiae); MCM2 minichromosome maintenance deficient 2, mitotin; MCM2_HUMAN; MGC10606; Minichromosome maintenance complex component 2; Minichromosome maintenance deficient 2 (mitotin); Minichromosome maintenance deficient 2 mitotin; Minichromosome maintenance protein 2; Minichromosome maintenance protein 2 homolog; Mitotin; Nuclear protein BM28; OTTHUMP00000216047; OTTHUMP00000216050;

Immunogens

Immunogen:
Uniprot:

P49736(MCM2_HUMAN) >>Visit HPA database.

Gene(ID):
MCM2(4171)
Description:
The minichromosome maintenance (MCM) 2-7 proteins are a family of six related proteins required for the initiation and elongation of DNA replication. MCM2-7 bind together to form the heterohexameric MCM complex that is thought to act as a replicative helicase at the DNA replication fork (1-5). This complex is also a key component of the pre-replication complex (pre-RC) (reviewed in 1). Cdc6 and CDT1 recruit the MCM complex to the origin recognition complex (ORC) during late mitosis/early G1 phase forming the pre-RC and licensing the DNA for replication (reviewed in 2). Phosphorylation of the MCM2, MCM3, MCM4, and MCM6 subunits appears to regulate MCM complex activity and the initiation of DNA synthesis (6-8). MCM proteins are removed during DNA replication, causing chromatin to become unlicensed through inhibition of pre-RC reformation. Licensing of the chromatin permits the DNA to replicate only once per cell cycle, thereby helping to ensure that genetic alterations and malignant cell growth do not occur (reviewed in 3). Studies have shown that the MCM complex is involved in checkpoint control by protecting the structure of the replication fork and assisting in restarting replication by recruiting checkpoint proteins after arrest (reviewed in 3,9).
Sequence:
MAESSESFTMASSPAQRRRGNDPLTSSPGRSSRRTDALTSSPGRDLPPFEDESEGLLGTEGPLEEEEDGEELIGDGMERDYRAIPELDAYEAEGLALDDEDVEELTASQREAAERAMRQRDREAGRGLGRMRRGLLYDSDEEDEERPARKRRQVERATEDGEEDEEMIESIENLEDLKGHSVREWVSMAGPRLEIHHRFKNFLRTHVDSHGHNVFKERISDMCKENRESLVVNYEDLAAREHVLAYFLPEAPAELLQIFDEAALEVVLAMYPKYDRITNHIHVRISHLPLVEELRSLRQLHLNQLIRTSGVVTSCTGVLPQLSMVKYNCNKCNFVLGPFCQSQNQEVKPGSCPECQSAGPFEVNMEETIYQNYQRIRIQESPGKVAAGRLPRSKDAILLADLVDSCKPGDEIELTGIYHNNYDGSLNTANGFPVFATVILANHVAKKDNKVAVGELTDEDVKMITSLSKDQQIGEKIFASIAPSIYGHEDIKRGLALALFGGEPKNPGGKHKVRGDINVLLCGDPGTAKSQFLKYIEKVSSRAIFTTGQGASAVGLTAYVQRHPVSREWTLEAGALVLADRGVCLIDEFDKMNDQDRTSIHEAMEQQSISISKAGIVTSLQARCTVIAAANPIGGRYDPSLTFSENVDLTEPIISRFDILCVVRDTVDPVQDEMLARFVVGSHVRHHPSNKEEEGLANGSAAEPAMPNTYGVEPLPQEVLKKYIIYAKERVHPKLNQMDQDKVAKMYSDLRKESMATGSIPITVRHIESMIRMAEAHARIHLRDYVIEDDVNMAIRVMLESFIDTQKFSVMRSMRKTFARYLSFRRDNNELLLFILKQLVAEQVTYQRNRFGAQQDTIEVPEKDLVDKARQINIHNLSAFYDSELFRMNKFSHDLKRKMILQQF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Zebrafish
100
Rabbit
100
Xenopus
86
Sheep
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P49736 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S4 Phosphorylation P50613 (CDK7) , O00311 (CDC7)
S5 Phosphorylation P50613 (CDK7) , O00311 (CDC7)
S7 Phosphorylation P50613 (CDK7) , O00311 (CDC7)
T9 Phosphorylation
S12 Phosphorylation
S13 Phosphorylation P24941 (CDK2) , P68400 (CSNK2A1) , O00311 (CDC7) , P50613 (CDK7)
T25 Phosphorylation
S26 Phosphorylation
S27 Phosphorylation P50613 (CDK7) , P24941 (CDK2) , P68400 (CSNK2A1) , O00311 (CDC7)
S31 Phosphorylation O00311 (CDC7)
S32 Phosphorylation
T35 Phosphorylation
T39 Phosphorylation
S40 Phosphorylation P68400 (CSNK2A1) , O00311 (CDC7) , P50613 (CDK7) , P24941 (CDK2)
S41 Phosphorylation O00311 (CDC7) , P24941 (CDK2) , P68400 (CSNK2A1) , P50613 (CDK7)
S53 Phosphorylation P68400 (CSNK2A1) , P24941 (CDK2) , P50613 (CDK7) , O00311 (CDC7)
T59 Phosphorylation P50613 (CDK7)
Y81 Phosphorylation
Y90 Phosphorylation
T106 Phosphorylation
S108 Phosphorylation P50613 (CDK7) , P24941 (CDK2) , P68400 (CSNK2A1) , Q13535 (ATR) , O00311 (CDC7)
R126 Methylation
Y137 Phosphorylation
S139 Phosphorylation P24941 (CDK2) , P68400 (CSNK2A1) , O00311 (CDC7) , P50613 (CDK7)
T158 Phosphorylation
S170 Phosphorylation
K178 Ubiquitination
K200 Ubiquitination
S209 Phosphorylation
K216 Acetylation
K216 Ubiquitination
S220 Phosphorylation O00311 (CDC7) , O14965 (AURKA)
K224 Ubiquitination
S229 Phosphorylation
Y234 Phosphorylation
R307 Methylation
C315 S-Nitrosylation
S381 Phosphorylation
K384 Ubiquitination
K394 Ubiquitination
K450 Ubiquitination
T457 Phosphorylation
K462 Ubiquitination
K469 Acetylation
K469 Ubiquitination
K476 Ubiquitination
S484 Phosphorylation
K492 Ubiquitination
T527 Phosphorylation
K529 Ubiquitination
K538 Ubiquitination
S566 Phosphorylation
K591 Ubiquitination
K613 Ubiquitination
R623 Methylation
R677 Methylation
K722 Ubiquitination
K728 Ubiquitination
K734 Ubiquitination
K742 Acetylation
K742 Ubiquitination
K745 Ubiquitination
R751 Methylation
K752 Ubiquitination
S754 Phosphorylation
T757 Phosphorylation
T763 Phosphorylation
S769 Phosphorylation
S801 Phosphorylation
T857 Phosphorylation
K863 Ubiquitination
K868 Ubiquitination
S878 Phosphorylation
K896 Acetylation
K896 Ubiquitination

Research Backgrounds

Function:

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division. Plays a role in terminally differentiated hair cells development of the cochlea and induces cells apoptosis.

PTMs:

Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5 (Probable). Interacts with DBF4 (By similarity). Interacts with KAT7. May interact with MCM10. Component of the replisome complex composed of at least DONSON, MCM2, MCM7, PCNA and TICRR.

Family&Domains:

Belongs to the MCM family.

Research Fields

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Genetic Information Processing > Replication and repair > DNA replication.

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