MTAP Antibody - #DF6197

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Product: MTAP Antibody
Catalog: DF6197
Description: Rabbit polyclonal antibody to MTAP
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Zebrafish, Bovine, Sheep, Rabbit, Chicken, Xenopus
Mol.Wt.: 31kDa; 31kD(Calculated).
Uniprot: Q13126
RRID: AB_2838163

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
MTAP Antibody detects endogenous levels of total MTAP.
RRID:
AB_2838163
Cite Format: Affinity Biosciences Cat# DF6197, RRID:AB_2838163.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

5' methylthioadenosine phosphorylase; 5''-methylthioadenosine phosphorylase; BDMF; c86fus; DMSFH; DMSMFH; Epididymis luminal protein 249; HEL 249; LGMBF; MeSAdo phosphorylase; Methylthioadenosine phosphorylase; MSAP; MTA phosphorylase; MTAP; MTAP_HUMAN; MTAPase; S methyl 5 thioadenosine phosphorylase; S methyl 5' thioadenosine phosphorylase; S-methyl-5''-thioadenosine phosphorylase;

Immunogens

Immunogen:
Uniprot:

Q13126(MTAP_HUMAN) >>Visit HPA database.

Gene(ID):
MTAP(4507)
Expression:
Q13126 MTAP_HUMAN:

Ubiquitously expressed.

Description:
MTAP is an enzyme that is essential for the salvage pathway for both adenine and methionine synthesis. MTAP catalyzes the cleavage of 5’-methylthioadenosine into adenine and 5-methylthio-D-ribose-1-phosphate. Adenine is then used to generate AMP whereas 5-methylthio-D-ribose-1-phosphate is converted into methionine (1,2). MTAP is expressed in all normal cells and tissues, although frequently lost in different human tumors including pancreatic adenocarcinoma, neuroendocrine tumors, non-small cell lung carcinoma and breast carcinoma. MTAP is usually codeleted with p16 (cdkN2a/ARF) (3-5). MTAP overexpression in breast cancer cells inhibits their ability to form colonies in soft agar, thereby implicating its function as a tumor suppressor (6).
Sequence:
MASGTTTTAVKIGIIGGTGLDDPEILEGRTEKYVDTPFGKPSDALILGKIKNVDCVLLARHGRQHTIMPSKVNYQANIWALKEEGCTHVIVTTACGSLREEIQPGDIVIIDQFIDRTTMRPQSFYDGSHSCARGVCHIPMAEPFCPKTREVLIETAKKLGLRCHSKGTMVTIEGPRFSSRAESFMFRTWGADVINMTTVPEVVLAKEAGICYASIAMATDYDCWKEHEEAVSVDRVLKTLKENANKAKSLLLTTIPQIGSTEWSETLHNLKNMAQFSVLLPRH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Pig
0
Horse
0
Dog
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13126 As Substrate

Site PTM Type Enzyme
S3 Phosphorylation
T5 Phosphorylation
T6 Phosphorylation
T7 Phosphorylation
T8 Phosphorylation
K32 Ubiquitination
K40 Ubiquitination
K49 Ubiquitination
R60 Methylation
K71 Ubiquitination
C131 S-Nitrosylation
K157 Ubiquitination
K166 Ubiquitination
S183 Phosphorylation
T188 Phosphorylation
R235 Methylation
K248 Ubiquitination

Research Backgrounds

Function:

Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.

Subcellular Location:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed.

Subunit Structure:

Homotrimer.

Family&Domains:

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.

Research Fields

· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

 

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