U2AF1 Antibody - #DF6195

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Product: U2AF1 Antibody
Catalog: DF6195
Description: Rabbit polyclonal antibody to U2AF1
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Zebrafish, Bovine, Dog, Chicken, Xenopus
Mol.Wt.: 28kDa; 28kD(Calculated).
Uniprot: Q01081
RRID: AB_2838161

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(80%), Bovine(100%), Dog(80%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
U2AF1 Antibody detects endogenous levels of total U2AF1.
RRID:
AB_2838161
Cite Format: Affinity Biosciences Cat# DF6195, RRID:AB_2838161.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DKFZp313J1712; FP793; Human U2 snRNP auxiliary factor small subunit9; RN; RNU2AF1; Splicing factor U2AF 35 kDa subunit; U2 auxiliary factor 35 kDa subunit; U2 small nuclear ribonucleoprotein auxillary factor 35 KD subunit; U2 small nuclear RNA auxiliary factor 1; U2 snRNP auxiliary factor small subunit; U2(RNU2) small nuclear RNA auxiliary factor 1; U2(RNU2) small nuclear RNA auxiliary factor binding protein; U2AF1; U2AF1_HUMAN; U2AF35; U2AFBP;

Immunogens

Immunogen:
Uniprot:

Q01081(U2AF1_HUMAN) >>Visit HPA database.

Gene(ID):
U2AF1(102724594)
Description:
U2 auxiliary factor (U2AF), comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. U2 (RNU2) small nuclear RNA auxiliary factor 1 (U2AF1,synonyms: RN, FP793, U2AF35, U2AFBP, RNU2AF1)is the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. The U2AF1 gene is localized to chromosome 21q22.3, which is the critical region for three diseases, progressive myoclonus epilepsy, autoimmune polyglandular disease type 1, and one form of bipolar affective disorder.
Sequence:
MAEYLASIFGTEKDKVNCSFYFKIGACRHGDRCSRLHNKPTFSQTIALLNIYRNPQNSSQSADGLRCAVSDVEMQEHYDEFFEEVFTEMEEKYGEVEEMNVCDNLGDHLVGNVYVKFRREEDAEKAVIDLNNRWFNGQPIHAELSPVTDFREACCRQYEMGECTRGGFCNFMHLKPISRELRRELYGRRRKKHRSRSRSRERRSRSRDRGRGGGGGGGGGGGGRERDRRRSRDRERSGRF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Xenopus
100
Chicken
100
Dog
80
Zebrafish
80
Pig
70
Horse
0
Sheep
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q01081 As Substrate

Site PTM Type Enzyme
Y4 Phosphorylation
S7 Phosphorylation
T11 Phosphorylation
K13 Acetylation
K13 Ubiquitination
K15 Sumoylation
K15 Ubiquitination
S19 Phosphorylation
K39 Methylation
S58 Phosphorylation
S59 Phosphorylation
S61 Phosphorylation
C67 S-Nitrosylation
S70 Phosphorylation
T87 Phosphorylation
K92 Sumoylation
Y93 Phosphorylation
C102 S-Nitrosylation
K116 Ubiquitination
K125 Ubiquitination
S145 Phosphorylation
R165 Methylation
K175 Acetylation
K175 Methylation
K175 Sumoylation
K175 Ubiquitination
S178 Phosphorylation
R179 Methylation
S204 Phosphorylation
S206 Phosphorylation
R209 Methylation
R211 Methylation
S231 Phosphorylation
S237 Phosphorylation

Research Backgrounds

Function:

Plays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron.

Subcellular Location:

Nucleus. Nucleus speckle.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts (via RS domain) with PHF5A (via N-terminus) (By similarity). Identified in the spliceosome C complex. Heterodimer with U2AF2. Interacts with ZRANB2.

Family&Domains:

The C-terminal SR-rich domain is required for interactions with SR proteins and the splicing regulators TRA and TRA2, and the N-terminal domain is required for formation of the U2AF1/U2AF2 heterodimer.

Belongs to the splicing factor SR family.

Research Fields

· Genetic Information Processing > Transcription > Spliceosome.

· Human Diseases > Infectious diseases: Bacterial > Shigellosis.

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