STAR Antibody - #DF6192

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Product: STAR Antibody
Catalog: DF6192
Description: Rabbit polyclonal antibody to STAR
Application: WB IHC
Reactivity: Human, Mouse, Rat, Duck
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 32kDa; 32kD(Calculated).
Uniprot: P49675
RRID: AB_2838158

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Duck
Prediction:
Pig(100%), Zebrafish(89%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(89%)
Clonality:
Polyclonal
Specificity:
STAR Antibody detects endogenous levels of total STAR.
RRID:
AB_2838158
Cite Format: Affinity Biosciences Cat# DF6192, RRID:AB_2838158.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Cholesterol trafficker; Luteinizing hormone induced protein; mitochondrial; Mitochondrial steroid acute regulatory protein; StAR; StAR related lipid transfer (START) domain containing 1; STAR_HUMAN; StARD1; START domain containing 1; START domain containing protein 1; START domain-containing protein 1; Steroid acute regulatory protein; Steroidogenic acute regulator; Steroidogenic acute regulatory protein; Steroidogenic acute regulatory protein mitochondrial;

Immunogens

Immunogen:
Uniprot:

P49675(STAR_HUMAN) >>Visit HPA database.

Gene(ID):
STAR(6770)
Expression:
P49675 STAR_HUMAN:

Expressed in gonads, adrenal cortex and kidney.

Description:
Steroidogenic acute regulatory protein (StAR) plays a significant role in cholesterol transport from the cytoplasmic outer membrane to the inner mitochondrial membrane (1). The 37 kDa precursor is cleaved to generate an active 28 kDa protein capable of facilitating cholesterol metabolism into pregnenolone (2,3). StAR is prevalently expressed in mitochondria of steroid-producing adrenal and gonadal tissue (3). Abnormalities in StAR gene expression are impacted in autosomal Lipoid Congenial Adrenal Hyperplasia (LCAH) resulting in defects in pregnenolone and cortisol synthesis (4). The mechanism of cholesterol binding to StAR has yet to be elucidated (4).
Sequence:
MLLATFKLCAGSSYRHMRNMKGLRQQAVMAISQELNRRALGGPTPSTWINQVRRRSSLLGSRLEETLYSDQELAYLQQGEEAMQKALGILSNQEGWKKESQQDNGDKVMSKVVPDVGKVFRLEVVVDQPMERLYEELVERMEAMGEWNPNVKEIKVLQKIGKDTFITHELAAEAAGNLVGPRDFVSVRCAKRRGSTCVLAGMATDFGNMPEQKGVIRAEHGPTCMVLHPLAGSPSKTKLTWLLSIDLKGWLPKSIINQVLSQTQVDFANHLRKRLESHPASEARC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
89
Zebrafish
89
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P49675 As Substrate

Site PTM Type Enzyme
S56 Phosphorylation Q13237 (PRKG2)
S57 Phosphorylation Q13237 (PRKG2)
S61 Phosphorylation
S100 Phosphorylation Q13237 (PRKG2)
S195 Phosphorylation
T204 Phosphorylation
S233 Phosphorylation P27361 (MAPK3)
K273 Acetylation

Research Backgrounds

Function:

Plays a key role in steroid hormone synthesis by enhancing the metabolism of cholesterol into pregnenolone. Mediates the transfer of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane where it is cleaved to pregnenolone.

Subcellular Location:

Mitochondrion.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in gonads, adrenal cortex and kidney.

Subunit Structure:

May interact with TSPO.

Research Fields

· Organismal Systems > Endocrine system > Ovarian steroidogenesis.

· Organismal Systems > Endocrine system > Aldosterone synthesis and secretion.

· Organismal Systems > Digestive system > Cholesterol metabolism.

References

1). The Curcumin Derivative, H10, Suppresses Hormone-Dependent Prostate Cancer by Inhibiting 17β-Hydroxysteroid Dehydrogenase Type 3. Frontiers in Pharmacology (PubMed: 32457626) [IF=5.6]

Application: WB    Species: mouse    Sample: LC540 (17b-HSD3) cells

FIGURE 3 | Effects of H10 on 17b-HSD3 in LC540 (17b-HSD3) cells. Effects of H10 on the production of (A) T and (B) P. (C) The expression of 17b-HSD3 mRNA.(D) The expression levels of the proteins in the T synthesis pathway were detected by western blotting following treatment with H10. The concentrations of H10 were 0.25, 0.5, and 1 mM, while the concentration of 3858858 (positive control) was 0.25 mM.
2). Effects of stocking density on ovarian development and maturation during the rearing period in Shan-ma ducks. POULTRY SCIENCE (PubMed: 35358924) [IF=4.4]

Application: WB    Species: Shan-maducks    Sample: ovarian

Figure 5.| Effects of stocking densities on protein levels of the ovarian steroid biosynthesis pathway. The protein expression levels of CYP19A1,3b-HSD, and StAR in ovarian tissue. Data are presented as the mean § SEM, n = 6. Adjacent letters indicate significant differences (P < 0.05) and interphase letters indicate extremely significant differences (P < 0.01).
3). Suppression of Notch Signaling Stimulates Progesterone Synthesis by Enhancing the Expression of NR5A2 and NR2F2 in Porcine Granulosa Cells. Genes (PubMed: 31978970) [IF=3.5]

Application: WB    Species: porcine    Sample: pGCs

Figure 2.| Effects of DAPT treatment on the expression of proteins involved in steroidogenesis in pGCs. (A) The gene expression of factors involved in cholesterol uptake (LDLR, VLDLR, SRB1, NPC1,SCP2) and progesterone synthesis (StAR, Cyp11a1, HSD3B); (B,C) The protein expression of StAR,Cyp11a1, and HSD3B

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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