SERPINA3 Antibody - #DF6185

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Product: SERPINA3 Antibody
Catalog: DF6185
Description: Rabbit polyclonal antibody to SERPINA3
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Rabbit
Mol.Wt.: 48kDa; 48kD(Calculated).
Uniprot: P01011
RRID: AB_2838151

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Rabbit(80%)
Clonality:
Polyclonal
Specificity:
SERPINA3 Antibody detects endogenous levels of total SERPINA3.
RRID:
AB_2838151
Cite Format: Affinity Biosciences Cat# DF6185, RRID:AB_2838151.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

SERPINA3; AACT; AACT_HUMAN; ACT; Alpha-1-antichymotrypsin His-Pro-less; Antichymotrypsin; Cell growth inhibiting gene 24/25 protein; Cell growth-inhibiting gene 24/25 protein; GIG24; GIG25; Growth inhibiting protein 24; Growth inhibiting protein 25; MGC88254; Serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase, antitrypsin) member 3; Serine (or cysteine) proteinase inhibitor clade A member 3; Serine proteinase inhibitor clade A member 3; Serpin A3; Serpin family A member 3; Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 3; SERPINA3;

Immunogens

Immunogen:
Uniprot:

P01011(AACT_HUMAN) >>Visit HPA database.

Gene(ID):
SERPINA3(12)
Expression:
P01011 AACT_HUMAN:

Plasma. Synthesized in the liver. Like the related alpha-1-antitrypsin, its concentration increases in the acute phase of inflammation or infection. Found in the amyloid plaques from the hippocampus of Alzheimer disease brains.

Description:
The protein encoded by this gene is a plasma protease inhibitor and member of the serine protease inhibitor class. Polymorphisms in this protein appear to be tissue specific and influence protease targeting. Variations in this protein's sequence have been implicated in Alzheimer's disease, and deficiency of this protein has been associated with liver disease. Mutations have been identified in patients with Parkinson disease and chronic obstructive pulmonary disease. [provided by RefSeq, Jul 2008]
Sequence:
MERMLPLLALGLLAAGFCPAVLCHPNSPLDEENLTQENQDRGTHVDLGLASANVDFAFSLYKQLVLKAPDKNVIFSPLSISTALAFLSLGAHNTTLTEILKGLKFNLTETSEAEIHQSFQHLLRTLNQSSDELQLSMGNAMFVKEQLSLLDRFTEDAKRLYGSEAFATDFQDSAAAKKLINDYVKNGTRGKITDLIKDLDSQTMMVLVNYIFFKAKWEMPFDPQDTHQSRFYLSKKKWVMVPMMSLHHLTIPYFRDEELSCTVVELKYTGNASALFILPDQDKMEEVEAMLLPETLKRWRDSLEFREIGELYLPKFSISRDYNLNDILLQLGIEEAFTSKADLSGITGARNLAVSQVVHKAVLDVFEEGTEASAATAVKITLLSALVETRTIVRFNRPFLMIIVPTDTQNIFFMSKVTNPKQA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
80
Horse
70
Dog
70
Xenopus
67
Bovine
44
Pig
0
Sheep
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P01011 As Substrate

Site PTM Type Enzyme
N33 N-Glycosylation
T35 Phosphorylation
N93 N-Glycosylation
N106 N-Glycosylation
N127 N-Glycosylation
K158 Acetylation
K177 Acetylation
N186 N-Glycosylation
K191 Acetylation
S201 Phosphorylation
T203 Phosphorylation
K235 Acetylation
K236 Acetylation
K237 Acetylation
C261 S-Nitrosylation
N271 N-Glycosylation
S273 Phosphorylation
T295 Phosphorylation
S302 Phosphorylation
T338 Phosphorylation
T347 O-Glycosylation
T347 Phosphorylation
S384 Phosphorylation
K416 Acetylation
K421 Acetylation

Research Backgrounds

Function:

Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2.

PTMs:

N- and O-glycosylated.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Plasma. Synthesized in the liver. Like the related alpha-1-antitrypsin, its concentration increases in the acute phase of inflammation or infection. Found in the amyloid plaques from the hippocampus of Alzheimer disease brains.

Subunit Structure:

Interacts with DNAJC1.

Family&Domains:

The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.

Belongs to the serpin family.

References

1). Serpina3n/serpina3 alleviates cyclophosphamide-induced interstitial cystitis by activating the Wnt/β-catenin signal. International Urology and Nephrology, 2023 (PubMed: 37594700) [IF=2.0]

Application: WB    Species: Human    Sample:

Fig. 1 Serpina3/serpina3n expression was down-regulated in IC/BPS. A Assessment of the pathological changes in human bladder tissues by HE staining (× 200). B Evaluation of mast-cell infiltration in human bladder tissues using toluidine blue staining (× 200). C, D The mRNA and protein levels of serpina3 in the bladder tissues from IC/BPS patients and healthy individuals were tested by qPCR and western blotting. E Assessment of the pathological changes in mouse bladder tissues by HE staining (× 200). F Evaluation of mast-cell infiltration in mouse bladder tissues using toluidine blue staining (× 200). G, H The mRNA and protein levels of serpina3 in the bladder tissues from IC/BPS patients and healthy individuals were tested by qPCR and western blotting. (*P 

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