ADA Antibody - #DF6184

(3)  
Product: ADA Antibody
Catalog: DF6184
Description: Rabbit polyclonal antibody to ADA
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 41kDa; 41kD(Calculated).
Uniprot: P00813
RRID: AB_2838150

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(91%), Bovine(92%), Horse(92%), Sheep(100%), Rabbit(100%), Dog(83%), Chicken(91%), Xenopus(82%)
Clonality:
Polyclonal
Specificity:
ADA Antibody detects endogenous levels of total ADA.
RRID:
AB_2838150
Cite Format: Affinity Biosciences Cat# DF6184, RRID:AB_2838150.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ada; ADA_HUMAN; ADA1; Adenosine aminohydrolase; Adenosine deaminase;

Immunogens

Immunogen:
Uniprot:

P00813(ADA_HUMAN) >>Visit HPA database.

Gene(ID):
ADA(100)
Expression:
P00813 ADA_HUMAN:

Found in all tissues, occurs in large amounts in T-lymphocytes (PubMed:20959412). Expressed at the time of weaning in gastrointestinal tissues.

Description:
This gene encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine. Various mutations have been described for this gene and have been linked to human diseases. Deficiency in this enzyme causes a form of severe combined immunodeficiency disease (SCID), in which there is dysfunction of both B and T lymphocytes with impaired cellular immunity and decreased production of immunoglobulins, whereas elevated levels of this enzyme have been associated with congenital hemolytic anemia. [provided by RefSeq, Jul 2008]
Sequence:
MAQTPAFDKPKVELHVHLDGSIKPETILYYGRRRGIALPANTAEGLLNVIGMDKPLTLPDFLAKFDYYMPAIAGCREAIKRIAYEFVEMKAKEGVVYVEVRYSPHLLANSKVEPIPWNQAEGDLTPDEVVALVGQGLQEGERDFGVKARSILCCMRHQPNWSPKVVELCKKYQQQTVVAIDLAGDETIPGSSLLPGHVQAYQEAVKSGIHRTVHAGEVGSAEVVKEAVDILKTERLGHGYHTLEDQALYNRLRQENMHFEICPWSSYLTGAWKPDTEHAVIRLKNDQANYSLNTDDPLIFKSTLDTDYQMTKRDMGFTEEEFKRLNINAAKSSFLPEDEKRELLDLLYKAYGMPPSASAGQNL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Sheep
100
Rabbit
100
Horse
92
Bovine
92
Zebrafish
91
Chicken
91
Dog
83
Xenopus
82
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P00813 As Substrate

Site PTM Type Enzyme
T4 Phosphorylation
K9 Acetylation
K9 Ubiquitination
K23 Ubiquitination
T26 Phosphorylation
Y29 Phosphorylation
Y30 Phosphorylation
K54 Acetylation
K54 Ubiquitination
Y67 Phosphorylation
Y84 Phosphorylation
K90 Ubiquitination
K92 Ubiquitination
K111 Ubiquitination
K147 Ubiquitination
S162 Phosphorylation
K164 Acetylation
K164 Ubiquitination
K170 Acetylation
K170 Ubiquitination
K171 Ubiquitination
K206 Ubiquitination
K225 Ubiquitination
K232 Acetylation
K232 Ubiquitination
Y240 Phosphorylation
Y249 Phosphorylation
K273 Ubiquitination
K284 Ubiquitination
Y290 Phosphorylation
S291 Phosphorylation
K301 Ubiquitination
Y308 Phosphorylation
K312 Acetylation
K312 Ubiquitination
K323 Acetylation
K323 Ubiquitination
K331 Ubiquitination
K340 Ubiquitination
Y348 Phosphorylation
Y351 Phosphorylation
S358 Phosphorylation

Research Backgrounds

Function:

Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion. Enhances dendritic cell immunogenicity by affecting dendritic cell costimulatory molecule expression and cytokines and chemokines secretion (By similarity). Enhances CD4+ T-cell differentiation and proliferation. Acts as a positive modulator of adenosine receptors ADORA1 and ADORA2A, by enhancing their ligand affinity via conformational change. Stimulates plasminogen activation. Plays a role in male fertility. Plays a protective role in early postimplantation embryonic development (By similarity).

Subcellular Location:

Cell membrane>Peripheral membrane protein>Extracellular side. Cell junction. Cytoplasmic vesicle lumen. Cytoplasm. Lysosome.
Note: Colocalized with DPP4 at the cell surface.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Found in all tissues, occurs in large amounts in T-lymphocytes. Expressed at the time of weaning in gastrointestinal tissues.

Subunit Structure:

Interacts with DPP4 (via extracellular domain). Interacts with PLG (via Kringle 4 domain); the interaction stimulates PLG activation when in complex with DPP4.

Family&Domains:

Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family.

Research Fields

· Human Diseases > Immune diseases > Primary immunodeficiency.

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.