TPMT Antibody - #DF6183

(3)  
Product: TPMT Antibody
Catalog: DF6183
Description: Rabbit polyclonal antibody to TPMT
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Rabbit
Mol.Wt.: 28kDa, 35 kDa; 28kD(Calculated).
Uniprot: P51580
RRID: AB_2838149

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(83%), Horse(91%), Rabbit(80%)
Clonality:
Polyclonal
Specificity:
TPMT Antibody detects endogenous levels of total TPMT.
RRID:
AB_2838149
Cite Format: Affinity Biosciences Cat# DF6183, RRID:AB_2838149.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

HGNC:12014; S adenosyl L methionine thiopurine S methyltransferase; Thiopurine methyltransferase; Thiopurine S methyltransferase; Thiopurine S-methyltransferase; TPMT; TPMT_HUMAN;

Immunogens

Immunogen:
Uniprot:

P51580(TPMT_HUMAN) >>Visit HPA database.

Gene(ID):
TPMT(7172)
Description:
This gene encodes the enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine are used as chemotherapeutic agents. Genetic polymorphisms that affect this enzymatic activity are correlated with variations in sensitivity and toxicity to such drugs within individuals. A pseudogene for this locus is located on chromosome 18q. [provided by RefSeq, Jul 2008]
Sequence:
MDGTRTSLDIEEYSDTEVQKNQVLTLEEWQDKWVNGKTAFHQEQGHQLLKKHLDTFLKGKSGLRVFFPLCGKAVEMKWFADRGHSVVGVEISELGIQEFFTEQNLSYSEEPITEIPGTKVFKSSSGNISLYCCSIFDLPRTNIGKFDMIWDRGALVAINPGDRKCYADTMFSLLGKKFQYLLCVLSYDPTKHPGPPFYVPHAEIERLFGKICNIRCLEKVDAFEERHKSWGIDCLFEKLYLLTEK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
91
Bovine
83
Rabbit
80
Sheep
75
Dog
70
Pig
58
Zebrafish
45
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P51580 As Substrate

Site PTM Type Enzyme
T6 Phosphorylation
S7 Phosphorylation
Y13 Phosphorylation
S14 Phosphorylation
T16 Phosphorylation
T25 Phosphorylation
K32 Acetylation
K32 Ubiquitination
K37 Ubiquitination
K50 Ubiquitination
K51 Ubiquitination
K58 Acetylation
K58 Ubiquitination
K77 Acetylation
Y166 Phosphorylation
K176 Ubiquitination
K210 Ubiquitination
K219 Ubiquitination
K228 Ubiquitination
S229 Phosphorylation

Research Backgrounds

Function:

Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine (also called mercaptopurine, 6-MP or its brand name Purinethol) and 6-thioguanine (also called tioguanine or 6-TG) using S-adenosyl-L-methionine as the methyl donor. TPMT activity modulates the cytotoxic effects of thiopurine prodrugs. A natural substrate for this enzyme has yet to be identified.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Monomer.

Family&Domains:

Belongs to the class I-like SAM-binding methyltransferase superfamily. TPMT family.

Research Fields

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.

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