PPIA Antibody - #DF6175

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Product: PPIA Antibody
Catalog: DF6175
Description: Rabbit polyclonal antibody to PPIA
Application: WB IHC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Bovine, Horse, Sheep, Rabbit
Mol.Wt.: 18kDa; 18kD(Calculated).
Uniprot: P62937
RRID: AB_2838142

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(83%)
Clonality:
Polyclonal
Specificity:
PPIA Antibody detects endogenous levels of total PPIA.
RRID:
AB_2838142
Cite Format: Affinity Biosciences Cat# DF6175, RRID:AB_2838142.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Cyclophilin A; Cyclophilin; CyclophilinA; Cyclosporin A binding protein; Cyclosporin A-binding protein; CYPA; CYPH; Epididymis secretory sperm binding protein Li 69p; HEL S 69p; MGC117158; MGC12404; MGC23397; Peptidyl prolyl cis trans isomerase A; Peptidyl-prolyl cis-trans isomerase A; Peptidylprolyl isomerase A (cyclophilin A); Peptidylprolyl isomerase A; PPIA; PPIA protein; PPIA_HUMAN; PPIase A; Rotamase A; RotamaseA; T cell cyclophilin;

Immunogens

Immunogen:
Uniprot:

P62937(PPIA_HUMAN) >>Visit HPA database.

Gene(ID):
PPIA(5478)
Description:
Cyclophilins are a highly conserved family of peptidylprolylcis-trans-isomerases (PPIA) that are targets of the immunosuppressant drug cyclosporin A (CsA) (1,2). The complex of cyclophilin and CsA can bind to and inhibit calcineurin which leads to inhibition of the transcription factor NFAT and decreased production of cytokines (3,4). As isomerases, cyclophilins have been proposed to aid in protein folding. Cyclophilin A can bind to the p55 Gag protein of HIV and appears necessary for HIV infection (5,6). There is also some evidence that cyclophilins have nuclease activity and play a role in apoptosis (7).
Sequence:
MVNPTVFFDIAVDGEPLGRVSFELFADKVPKTAENFRALSTGEKGFGYKGSCFHRIIPGFMCQGGDFTRHNGTGGKSIYGEKFEDENFILKHTGPGILSMANAGPNTNGSQFFICTAKTEWLDGKHVVFGKVKEGMNIVEAMERFGSRNGKTSKKITIADCGQLE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Rabbit
83
Chicken
75
Xenopus
58
Dog
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P62937 As Substrate

Site PTM Type Enzyme
M1 Acetylation
V2 Acetylation
T5 Phosphorylation
S21 Phosphorylation
K28 Acetylation
K28 Ubiquitination
K31 Acetylation
K31 Sumoylation
K31 Ubiquitination
S40 Phosphorylation
K44 Acetylation
K44 Sumoylation
K44 Ubiquitination
Y48 Phosphorylation
K49 Acetylation
K49 Methylation
K49 Sumoylation
K49 Ubiquitination
S51 Phosphorylation
C52 S-Nitrosylation
T68 Phosphorylation
K76 Acetylation
K76 Sumoylation
K76 Ubiquitination
S77 Phosphorylation
Y79 Phosphorylation
K82 Acetylation
K82 Sumoylation
K82 Ubiquitination
K91 Acetylation
K91 Methylation
K91 Ubiquitination
T93 Phosphorylation
S99 Phosphorylation
T107 Phosphorylation
S110 Phosphorylation
T116 Phosphorylation
K118 Acetylation
K118 Sumoylation
K118 Ubiquitination
K125 Acetylation
K125 Methylation
K125 Sumoylation
K125 Ubiquitination
K131 Acetylation
K131 Ubiquitination
K133 Acetylation
K133 Sumoylation
K133 Ubiquitination
S147 Phosphorylation
K151 Ubiquitination
K155 Acetylation
K155 Sumoylation
K155 Ubiquitination
T157 Phosphorylation
C161 S-Nitrosylation

Research Backgrounds

Function:

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.

PTMs:

Acetylation at Lys-125 markedly inhibits catalysis of cis to trans isomerization and stabilizes cis rather than trans forms of the HIV-1 capsid. PPIA acetylation also antagonizes the immunosuppressive effects of cyclosporine by inhibiting the sequential steps of cyclosporine binding and calcineurin inhibition.

Subcellular Location:

Cytoplasm. Secreted.
Note: Secretion occurs in response to oxidative stress in vascular smooth muscle through a vesicular secretory pathway that involves actin remodeling and myosin II activation, and mediates ERK1/2 activation.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with protein phosphatase PPP3CA/calcineurin A.

(Microbial infection) Interacts with HIV-1 capsid protein.

Family&Domains:

Belongs to the cyclophilin-type PPIase family. PPIase A subfamily.

Research Fields

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

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