GRP94 Antibody | Affinity Biosciences

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Western blot analysis of HSP90B1 expression in Rat muscle lysate.

Product:	GRP94 Antibody
Catalog:	DF6171
Description:	Rabbit polyclonal antibody to GRP94
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Sheep, Rabbit, Dog
Mol.Wt.:	92kDa; 92kD(Calculated).
Uniprot:	P14625
RRID:	AB_2838138

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(88%), Sheep(88%), Rabbit(88%), Dog(88%)

Clonality:

Polyclonal

Specificity:

GRP94 Antibody detects endogenous levels of total GRP94.

RRID:

AB_2838138
Cite Format: Affinity Biosciences Cat# DF6171, RRID:AB_2838138.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

94 kDa glucose regulated protein; 94 kDa glucose-regulated protein; ECGP; Endoplasmin; Endothelial cell (HBMEC) glycoprotein; ENPL_HUMAN; Glucose regulated protein 94kDa; gp96; gp96 homolog; GRP 94; GRP-94; Heat shock protein 90 kDa beta member 1; heat shock protein 90kDa beta (Grp94), member 1; Heat shock protein, 90 kDa, beta, 1; HSP90B1; Stress inducible tumor rejection antigen GP96; TRA1; tumor rejection antigen (gp96) 1; Tumor rejection antigen 1; Tumor rejection antigen gp96; Tumor rejection antigen-1 (gp96);

Immunogens

Immunogen:

Uniprot:

P14625(ENPL_HUMAN) >>Visit HPA database.

Gene(ID):

HSP90B1(7184)

Description:

Secretory proteins are synthesized on polysomes and translocated into the endoplasmic reticulum (ER). Inside ER, these proteins are often modified by disulfide bond formation, amino-linked glycosylation and folding. The ER contains a pool of molecular chaperones, including Grp94, to help proteins fold properly. Grp94 is a glucose-regulated protein (1) with sequence homology to Hsp90 (2). In addition to its role to help some secretory proteins fold to their correct conformation (3), studies suggest that Grp94 derived from cancer cells also induces anti-tumor immune responses in mouse tumor models (4, 5). One reason for this tumor immunogenicity is that Grp94 binds to the peptides from proteins in cancer cells and can therefore present these peptides as tumor antigens (6). Furthermore, Grp94 has also been shown to induce maturation of dendritic cells (7). Taken together, Grp94 functions both as a tumor-specific antigen and as an activator of antigen-presenting cells to elicit immunity to cancers (8).

Sequence:

P14625 ENPL_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MRALWVLGLCCVLLTFGSVRADDEVDVDGTVEEDLGKSREGSRTDDEVVQREEEAIQLDGLNASQIRELREKSEKFAFQAEVNRMMKLIINSLYKNKEIFLRELISNASDALDKIRLISLTDENALSGNEELTVKIKCDKEKNLLHVTDTGVGMTREELVKNLGTIAKSGTSEFLNKMTEAQEDGQSTSELIGQFGVGFYSAFLVADKVIVTSKHNNDTQHIWESDSNEFSVIADPRGNTLGRGTTITLVLKEEASDYLELDTIKNLVKKYSQFINFPIYVWSSKTETVEEPMEEEEAAKEEKEESDDEAAVEEEEEEKKPKTKKVEKTVWDWELMNDIKPIWQRPSKEVEEDEYKAFYKSFSKESDDPMAYIHFTAEGEVTFKSILFVPTSAPRGLFDEYGSKKSDYIKLYVRRVFITDDFHDMMPKYLNFVKGVVDSDDLPLNVSRETLQQHKLLKVIRKKLVRKTLDMIKKIADDKYNDTFWKEFGTNIKLGVIEDHSNRTRLAKLLRFQSSHHPTDITSLDQYVERMKEKQDKIYFMAGSSRKEAESSPFVERLLKKGYEVIYLTEPVDEYCIQALPEFDGKRFQNVAKEGVKFDESEKTKESREAVEKEFEPLLNWMKDKALKDKIEKAVVSQRLTESPCALVASQYGWSGNMERIMKAQAYQTGKDISTNYYASQKKTFEINPRHPLIRDMLRRIKEDEDDKTVLDLAVVLFETATLRSGYLLPDTKAYGDRIERMLRLSLNIDPDAKVEEEPEEEPEETAEDTTEDTEQDEDEEMDVGTDEEEETAKESTAEKDEL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

Sheep

Dog

Rabbit

Horse

Xenopus

Chicken

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P14625 As Substrate

P14625

Site	PTM Type	Source
S42	Phosphorylation	Uniprot
T44	Phosphorylation	Uniprot
N62	N-Glycosylation	Uniprot
S64	Phosphorylation	Uniprot
K72	Ubiquitination	Uniprot
K75	Acetylation	Uniprot
K75	Ubiquitination	Uniprot
S92	Phosphorylation	Uniprot
Y94	Phosphorylation	Uniprot
K95	Ubiquitination	Uniprot
K97	Acetylation	Uniprot
K97	Ubiquitination	Uniprot
S106	Phosphorylation	Uniprot
N107	N-Glycosylation	Uniprot
S109	Phosphorylation	Uniprot
K114	Acetylation	Uniprot
K114	Ubiquitination	Uniprot
S127	Phosphorylation	Uniprot
T133	Phosphorylation	Uniprot
K135	Ubiquitination	Uniprot
K140	Acetylation	Uniprot
K142	Ubiquitination	Uniprot
T148	Phosphorylation	Uniprot
T150	Phosphorylation	Uniprot
K161	Ubiquitination	Uniprot
T165	Phosphorylation	Uniprot
K168	Ubiquitination	Uniprot
S169	Phosphorylation	Uniprot
T171	Phosphorylation	Uniprot
S172	Phosphorylation	Uniprot
K177	Methylation	Uniprot
T179	Phosphorylation	Uniprot
S187	Phosphorylation	Uniprot
Y200	Phosphorylation	Uniprot
S201	Phosphorylation	Uniprot
N217	N-Glycosylation	Uniprot
S225	Phosphorylation	Uniprot
S227	Phosphorylation	Uniprot
K252	Ubiquitination	Uniprot
S256	Phosphorylation	Uniprot
Y258	Phosphorylation	Uniprot
K265	Ubiquitination	Uniprot
K270	Ubiquitination	Uniprot
Y280	Phosphorylation	Uniprot
T288	Phosphorylation	Uniprot
S306	Phosphorylation	Uniprot
K340	Ubiquitination	Uniprot
S347	Phosphorylation	Uniprot
K348	Ubiquitination	Uniprot
K356	Ubiquitination	Uniprot
K360	Ubiquitination	Uniprot
S385	Phosphorylation	Uniprot
T391	Phosphorylation	Uniprot
S392	Phosphorylation	Uniprot
R395	Methylation	Uniprot
Y401	Phosphorylation	Uniprot
S403	Phosphorylation	Uniprot
K404	Acetylation	Uniprot
K404	Ubiquitination	Uniprot
K405	Ubiquitination	Uniprot
Y408	Phosphorylation	Uniprot
K410	Ubiquitination	Uniprot
Y429	Phosphorylation	Uniprot
K434	Ubiquitination	Uniprot
S439	Phosphorylation	Uniprot
N445	N-Glycosylation	Uniprot
S447	Phosphorylation	Uniprot
K455	Ubiquitination	Uniprot
K458	Ubiquitination	Uniprot
K467	Ubiquitination	Uniprot
T468	Phosphorylation	Uniprot
K473	Ubiquitination	Uniprot
K479	Acetylation	Uniprot
K479	Ubiquitination	Uniprot
K486	Methylation	Uniprot
K486	Ubiquitination	Uniprot
K493	Ubiquitination	Uniprot
S501	Phosphorylation	Uniprot
T504	Phosphorylation	Uniprot
R511	Methylation	Uniprot
S515	Phosphorylation	Uniprot
T519	Phosphorylation	Uniprot
Y527	Phosphorylation	Uniprot
S544	Phosphorylation	Uniprot
S545	Phosphorylation	Uniprot
S551	Phosphorylation	Uniprot
S552	Phosphorylation	Uniprot
K561	Ubiquitination	Uniprot
K586	Acetylation	Uniprot
K586	Sumoylation	Uniprot
K586	Ubiquitination	Uniprot
K593	Ubiquitination	Uniprot
T604	Phosphorylation	Uniprot
K613	Acetylation	Uniprot
K613	Ubiquitination	Uniprot
K623	Ubiquitination	Uniprot
K633	Ubiquitination	Uniprot
T641	Phosphorylation	Uniprot
S643	Phosphorylation	Uniprot
Y652	Phosphorylation	Uniprot
K663	Methylation	Uniprot
K663	Ubiquitination	Uniprot
Y667	Phosphorylation	Uniprot
T669	Phosphorylation	Uniprot
K671	Ubiquitination	Uniprot
S674	Phosphorylation	Uniprot
T675	Phosphorylation	Uniprot
Y677	Phosphorylation	Uniprot
Y678	Phosphorylation	Uniprot
S680	Phosphorylation	Uniprot
K682	Acetylation	Uniprot
K682	Ubiquitination	Uniprot
K683	Ubiquitination	Uniprot
T684	Phosphorylation	Uniprot
R690	Methylation	Uniprot
Y727	Phosphorylation	Uniprot
K733	Ubiquitination	Uniprot
Y735	Phosphorylation	Uniprot
R738	Methylation	Uniprot
R744	Methylation	Uniprot
S746	Phosphorylation	Uniprot
T774	Phosphorylation	Uniprot
T786	Phosphorylation	Uniprot
T792	Phosphorylation	Uniprot

Research Backgrounds

P14625_ENPL_HUMAN

Function:

Molecular chaperone that functions in the processing and transport of secreted proteins (By similarity). When associated with CNPY3, required for proper folding of Toll-like receptors (By similarity). Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity (By similarity).

Subcellular Location:

Endoplasmic reticulum lumen. Sarcoplasmic reticulum lumen. Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Subunit Structure:

Homodimer; disulfide-linked. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5 (By similarity). Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with AIMP1; regulates its retention in the endoplasmic reticulum. Interacts with OS9. Interacts with CNPY3. This interaction is disrupted in the presence of ATP (By similarity). Interacts with TLR4 and TLR9, but not with TLR3. Interacts with MZB1 in a calcium-dependent manner (By similarity). Interacts with METTL23.

Family&Domains:

Belongs to the heat shock protein 90 family.

Research Fields

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Prostate cancer. (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway. (View pathway)

· Organismal Systems > Endocrine system > Estrogen signaling pathway. (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone synthesis.

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GRP94 Antibody - #DF6171