MSH6 Antibody | Affinity Biosciences

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Western blot analysis of Hela whole cell lysates, using MSH6 Antibody.

Product:	MSH6 Antibody
Catalog:	DF6165
Description:	Rabbit polyclonal antibody to MSH6
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	153kDa; 153kD(Calculated).
Uniprot:	P52701
RRID:	AB_2838132

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(83%), Bovine(91%), Horse(100%), Sheep(91%), Rabbit(82%), Dog(91%)

Clonality:

Polyclonal

Specificity:

MSH6 Antibody detects endogenous levels of total MSH6.

RRID:

AB_2838132
Cite Format: Affinity Biosciences Cat# DF6165, RRID:AB_2838132.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

DNA mismatch repair protein Msh6; G/T mismatch binding protein; G/T mismatch-binding protein; GTBP; GTMBP; hMSH6; HNPCC 5; HNPCC5; HSAP; MSH 6; MSH6; MSH6_HUMAN; mutS (E. coli) homolog 6; MutS alpha 160 kDa subunit; MutS homolog 6 (E. coli); mutS homolog 6; MutS-alpha 160 kDa subunit; p160; Sperm associated protein;

Immunogens

Immunogen:

Uniprot:

P52701(MSH6_HUMAN) >>Visit HPA database.

Gene(ID):

MSH6(2956)

Description:

The DNA mismatch repair system (MMR) repairs post-replication DNA, inhibits recombination between nonidentical DNA sequences, and induces both checkpoint and apoptotic responses following certain types of DNA damage (1). MSH2 (MutS homologue 2) forms the hMutS-α dimer with MSH6 and is an essential component of the mismatch repair process. hMutS-α is part of the BRCA1-associated surveillance complex (BASC), a complex that also contains BRCA1, MLH1, ATM, BLM, PMS2 proteins, and the Rad50-Mre11-NBS1 complex (2). Mutations in MSH6 and other MMR proteins have been found in a large proportion of hereditary nonpolyposis colorectal cancer (Lynch Syndrome), the most common form of inherited colorectal cancer in the Western world (3). Mutations in MSH6 have been shown to occur in glioblastoma in response to temozolomide therapy and to promote temozolomide resistance (4).

Sequence:

P52701 MSH6_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSRQSTLYSFFPKSPALSDANKASARASREGGRAAAAPGASPSPGGDAAWSEAGPGPRPLARSASPPKAKNLNGGLRRSVAPAAPTSCDFSPGDLVWAKMEGYPWWPCLVYNHPFDGTFIREKGKSVRVHVQFFDDSPTRGWVSKRLLKPYTGSKSKEAQKGGHFYSAKPEILRAMQRADEALNKDKIKRLELAVCDEPSEPEEEEEMEVGTTYVTDKSEEDNEIESEEEVQPKTQGSRRSSRQIKKRRVISDSESDIGGSDVEFKPDTKEEGSSDEISSGVGDSESEGLNSPVKVARKRKRMVTGNGSLKRKSSRKETPSATKQATSISSETKNTLRAFSAPQNSESQAHVSGGGDDSSRPTVWYHETLEWLKEEKRRDEHRRRPDHPDFDASTLYVPEDFLNSCTPGMRKWWQIKSQNFDLVICYKVGKFYELYHMDALIGVSELGLVFMKGNWAHSGFPEIAFGRYSDSLVQKGYKVARVEQTETPEMMEARCRKMAHISKYDRVVRREICRIITKGTQTYSVLEGDPSENYSKYLLSLKEKEEDSSGHTRAYGVCFVDTSLGKFFIGQFSDDRHCSRFRTLVAHYPPVQVLFEKGNLSKETKTILKSSLSCSLQEGLIPGSQFWDASKTLRTLLEEEYFREKLSDGIGVMLPQVLKGMTSESDSIGLTPGEKSELALSALGGCVFYLKKCLIDQELLSMANFEEYIPLDSDTVSTTRSGAIFTKAYQRMVLDAVTLNNLEIFLNGTNGSTEGTLLERVDTCHTPFGKRLLKQWLCAPLCNHYAINDRLDAIEDLMVVPDKISEVVELLKKLPDLERLLSKIHNVGSPLKSQNHPDSRAIMYEETTYSKKKIIDFLSALEGFKVMCKIIGIMEEVADGFKSKILKQVISLQTKNPEGRFPDLTVELNRWDTAFDHEKARKTGLITPKAGFDSDYDQALADIRENEQSLLEYLEKQRNRIGCRTIVYWGIGRNRYQLEIPENFTTRNLPEEYELKSTKKGCKRYWTKTIEKKLANLINAEERRDVSLKDCMRRLFYNFDKNYKDWQSAVECIAVLDVLLCLANYSRGGDGPMCRPVILLPEDTPPFLELKGSRHPCITKTFFGDDFIPNDILIGCEEEEQENGKAYCVLVTGPNMGGKSTLMRQAGLLAVMAQMGCYVPAEVCRLTPIDRVFTRLGASDRIMSGESTFFVELSETASILMHATAHSLVLVDELGRGTATFDGTAIANAVVKELAETIKCRTLFSTHYHSLVEDYSQNVAVRLGHMACMVENECEDPSQETITFLYKFIKGACPKSYGFNAARLANLPEEVIQKGHRKAREFEKMNQSLRLFREVCLASERSTVDAEAVHKLLTLIKEL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

Sheep

Dog

Pig

Rabbit

Xenopus

Chicken

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P52701 As Substrate

P52701

Site	PTM Type	Source
Y8	Phosphorylation	Uniprot
S9	Phosphorylation	Uniprot
S14	Phosphorylation	Uniprot
K22	Ubiquitination	Uniprot
R33	Methylation	Uniprot
S41	Phosphorylation	Uniprot
S43	Phosphorylation	Uniprot
S51	Phosphorylation	Uniprot
S63	Phosphorylation	Uniprot
S65	Phosphorylation	Uniprot
K70	Acetylation	Uniprot
S79	Phosphorylation	Uniprot
T86	Phosphorylation	Uniprot
S87	Phosphorylation	Uniprot
S91	Phosphorylation	Uniprot
S137	Phosphorylation	Uniprot
T139	Phosphorylation	Uniprot
R140	Methylation	Uniprot
S144	Phosphorylation	Uniprot
Y151	Phosphorylation	Uniprot
K155	Acetylation	Uniprot
K161	Ubiquitination	Uniprot
K169	Sumoylation	Uniprot
K169	Ubiquitination	Uniprot
S200	Phosphorylation	Uniprot
T212	Phosphorylation	Uniprot
T213	Phosphorylation	Uniprot
Y214	Phosphorylation	Uniprot
T216	Phosphorylation	Uniprot
S219	Phosphorylation	Uniprot
S227	Phosphorylation	Uniprot
T235	Phosphorylation	Uniprot
S252	Phosphorylation	Uniprot
S254	Phosphorylation	Uniprot
S256	Phosphorylation	Uniprot
S261	Phosphorylation	Uniprot
T269	Phosphorylation	Uniprot
S274	Phosphorylation	Uniprot
S275	Phosphorylation	Uniprot
S279	Phosphorylation	Uniprot
S280	Phosphorylation	Uniprot
S285	Phosphorylation	Uniprot
S292	Phosphorylation	Uniprot
T305	Phosphorylation	Uniprot
S309	Phosphorylation	Uniprot
K324	Ubiquitination	Uniprot
K334	Sumoylation	Uniprot
K334	Ubiquitination	Uniprot
S348	Phosphorylation	Uniprot
K374	Ubiquitination	Uniprot
K476	Ubiquitination	Uniprot
K479	Ubiquitination	Uniprot
T488	Phosphorylation	Uniprot
K498	Ubiquitination	Uniprot
K504	Acetylation	Uniprot
K504	Ubiquitination	Uniprot
K519	Ubiquitination	Uniprot
K537	Ubiquitination	Uniprot
K543	Ubiquitination	Uniprot
R577	Methylation	Uniprot
K598	Ubiquitination	Uniprot
K603	Ubiquitination	Uniprot
K610	Ubiquitination	Uniprot
K632	Ubiquitination	Uniprot
Y642	Phosphorylation	Uniprot
K646	Ubiquitination	Uniprot
K660	Ubiquitination	Uniprot
S668	Phosphorylation	Uniprot
K693	Ubiquitination	Uniprot
S702	Phosphorylation	Uniprot
Y709	Phosphorylation	Uniprot
K728	Ubiquitination	Uniprot
T767	Phosphorylation	Uniprot
K771	Ubiquitination	Uniprot
K813	Ubiquitination	Uniprot
K814	Ubiquitination	Uniprot
K824	Ubiquitination	Uniprot
S830	Phosphorylation	Uniprot
K833	Methylation	Uniprot
K833	Ubiquitination	Uniprot
K852	Ubiquitination	Uniprot
K854	Ubiquitination	Uniprot
K866	Ubiquitination	Uniprot
K870	Ubiquitination	Uniprot
K883	Ubiquitination	Uniprot
K888	Ubiquitination	Uniprot
T895	Phosphorylation	Uniprot
K896	Ubiquitination	Uniprot
T914	Phosphorylation	Uniprot
K920	Ubiquitination	Uniprot
K923	Ubiquitination	Uniprot
T924	Phosphorylation	Uniprot
T928	Phosphorylation	Uniprot
K930	Ubiquitination	Uniprot
S935	Phosphorylation	Uniprot
K957	Ubiquitination	Uniprot
K997	Sumoylation	Uniprot
K997	Ubiquitination	Uniprot
K1009	Ubiquitination	Uniprot
T1010	Phosphorylation	Uniprot
K1014	Ubiquitination	Uniprot
K1030	Ubiquitination	Uniprot
T1085	Phosphorylation	Uniprot
K1092	Methylation	Uniprot
K1092	Ubiquitination	Uniprot
K1101	Ubiquitination	Uniprot
K1126	Methylation	Uniprot
K1140	Ubiquitination	Uniprot
R1172	Methylation	Uniprot
K1240	Ubiquitination	Uniprot
K1291	Ubiquitination	Uniprot
K1296	Ubiquitination	Uniprot
K1315	Ubiquitination	Uniprot
K1325	Ubiquitination	Uniprot
K1352	Ubiquitination	Uniprot
K1358	Ubiquitination	Uniprot

Research Backgrounds

P52701_MSH6_HUMAN

Function:

Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3 (H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction.

PTMs:

The N-terminus is blocked.

Phosphorylated by PRKCZ, which may prevent MutS alpha degradation by the ubiquitin-proteasome pathway.

Subcellular Location:

Nucleus. Chromosome.
Note: Associates with H3K36me3 via its PWWP domain.

Subunit Structure:

Component of the DNA mismatch repair (MMR) complex composed at least of MSH2, MSH3, MSH6, PMS1 and MLH1. Heterodimer consisting of MSH2-MSH6 (MutS alpha). Forms a ternary complex with MutL alpha (MLH1-PMS1). Interacts with MCM9. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains.

(Microbial infection) Interacts with herpes simplex virus 1 protein UL12.

Family&Domains:

The PWWP domain specifically recognizes and binds trimethylated 'Lys-36' of histone H3 (H3K36me3).

Belongs to the DNA mismatch repair MutS family.

Research Fields

· Genetic Information Processing > Replication and repair > Mismatch repair.

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Colorectal cancer. (View pathway)

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MSH6 Antibody - #DF6165