WASP Antibody | Affinity Biosciences

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Western blot analysis of lovo cell lysates, using WAS Antibody.

Product:	WASP Antibody
Catalog:	DF6160
Description:	Rabbit polyclonal antibody to WASP
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Sheep, Rabbit, Dog, Xenopus
Mol.Wt.:	53kDa; 53kD(Calculated).
Uniprot:	P42768
RRID:	AB_2838127

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Sheep(88%), Rabbit(100%), Dog(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

WASP Antibody detects endogenous levels of total WASP.

RRID:

AB_2838127
Cite Format: Affinity Biosciences Cat# DF6160, RRID:AB_2838127.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Eczema thrombocytopenia; IMD2; SCNX; THC; THC1; Thrombocytopenia 1 (X linked); U42471; Was; WASp; WASP_HUMAN; Wiskott Aldrich syndrome (eczema thrombocytopenia); Wiskott Aldrich syndrome; Wiskott Aldrich syndrome protein; Wiskott-Aldrich syndrome protein;

Immunogens

Immunogen:

Uniprot:

P42768(WASP_HUMAN) >>Visit HPA database.

Gene(ID):

WAS(7454)

Expression:

P42768 WASP_HUMAN:

Expressed predominantly in the thymus. Also found, to a much lesser extent, in the spleen.

Description:

Wiskott-Aldrich syndrome proteins (WASPs) mediate actin dynamics by activating the Arp2/3 actin nucleation complex in response to activated Rho family GTPases. In mammals, five WASP family members have been described. Hematopoietic WASP and ubiquitously expressed N-WASP are autoinhibited in unstimulated cells. Upon stimulation they are activated by cdc42, which relieves the autoinhibition in conjunction with phosphatidyl inositol 4,5-bisphosphate. Three WAVE (Wasf, SCAR) family proteins are similar in sequence to WASP and N-WASP but lack the WASP/N-WASP autoinhibition domains and are indirectly activated by Rac (reviewed in 1). Both WASP and WAVE functions appear to be essential, as knockout of either N-WASP or Scar-2 in mice results in cardiac and neuronal defects and embryonic lethality (2,3). Loss of WASP results in immune system defects and fewer immune cells (4). WAVE-2 (WASF2) is widely distributed, while WAVE-1 and WAVE-3 are strongly expressed in brain (5). WAVE-3 may act as a tumor suppressor in neuroblastoma, a childhood disease of the sympathetic nervous system (6). Increased expression of WAVE-3 is seen in breast cancer, and studies in breast adenocarcinoma cells indicate that WAVE-3 regulates breast cancer progression, invasion and metastasis through the p38 mitogen-activated protein kinase (MAPK) pathway (7,8).

Sequence:

P42768 WASP_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSGGPMGGRPGGRGAPAVQQNIPSTLLQDHENQRLFEMLGRKCLTLATAVVQLYLALPPGAEHWTKEHCGAVCFVKDNPQKSYFIRLYGLQAGRLLWEQELYSQLVYSTPTPFFHTFAGDDCQAGLNFADEDEAQAFRALVQEKIQKRNQRQSGDRRQLPPPPTPANEERRGGLPPLPLHPGGDQGGPPVGPLSLGLATVDIQNPDITSSRYRGLPAPGPSPADKKRSGKKKISKADIGAPSGFKHVSHVGWDPQNGFDVNNLDPDLRSLFSRAGISEAQLTDAETSKLIYDFIEDQGGLEAVRQEMRRQEPLPPPPPPSRGGNQLPRPPIVGGNKGRSGPLPPVPLGIAPPPPTPRGPPPPGRGGPPPPPPPATGRSGPLPPPPPGAGGPPMPPPPPPPPPPPSSGNGPAPPPLPPALVPAGGLAPGGGRGALLDQIRQGIQLNKTPGAPESSALQPPPQSSEGLVGALMHVMQKRSRAIHSSDEGEDQAGDEDEDDEWDD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Dog

100

Xenopus

100

Rabbit

100

Sheep

Pig

Horse

Bovine

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P42768 As Substrate

P42768

Site	PTM Type	Enzyme	Source
S2	Phosphorylation		Uniprot
K76	Sumoylation		Uniprot
K76	Ubiquitination		Uniprot
K81	Ubiquitination		Uniprot
Y83	Phosphorylation		Uniprot
Y102	Phosphorylation	Q9UM73 (ALK)	Uniprot
K144	Sumoylation		Uniprot
K144	Ubiquitination		Uniprot
K147	Sumoylation		Uniprot
K147	Ubiquitination		Uniprot
S221	Phosphorylation		Uniprot
S234	Phosphorylation		Uniprot
K235	Ubiquitination		Uniprot
S277	Phosphorylation	Q07912 (TNK2)	Uniprot
T282	Phosphorylation		Uniprot
T286	Phosphorylation		Uniprot
S287	Phosphorylation		Uniprot
Y291	Phosphorylation	P07948 (LYN) , P06241 (FYN) , P00519 (ABL1) , Q07912 (TNK2) , Q06187 (BTK) , P06239 (LCK) , P08631 (HCK)	Uniprot
S320	Phosphorylation		Uniprot
R321	Methylation		Uniprot
R328	Methylation		Uniprot
R338	Methylation		Uniprot
T355	Phosphorylation		Uniprot
R357	Methylation		Uniprot
R364	Methylation		Uniprot
R377	Methylation		Uniprot
R431	Methylation		Uniprot
R439	Methylation		Uniprot
S483	Phosphorylation	P19784 (CSNK2A2) , P68400 (CSNK2A1)	Uniprot
S484	Phosphorylation	P68400 (CSNK2A1) , P19784 (CSNK2A2)	Uniprot

Research Backgrounds

P42768_WASP_HUMAN

Function:

Effector protein for Rho-type GTPases that regulates actin filament reorganization via its interaction with the Arp2/3 complex. Important for efficient actin polymerization. Possible regulator of lymphocyte and platelet function. Mediates actin filament reorganization and the formation of actin pedestals upon infection by pathogenic bacteria. In addition to its role in the cytoplasmic cytoskeleton, also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. Promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).

PTMs:

Phosphorylated at Tyr-291 by FYN and HCK, inducing WAS effector activity after TCR engagement. Phosphorylation at Tyr-291 enhances WAS activity in promoting actin polymerization and filopodia formation.

Subcellular Location:

Cytoplasm>Cytoskeleton. Nucleus.

Tissue Specificity:

Expressed predominantly in the thymus. Also found, to a much lesser extent, in the spleen.

Subunit Structure:

Binds the Arp2/3 complex. Interacts with CDC42, RAC, NCK, HCK, FYN, SRC kinase FGR, BTK, ABL1, PSTPIP1, WIP, and to the p85 subunit of PLC-gamma. Interacts (via C-terminus) with ALDOA. Interacts with NCK1 (via SH3 domains) (By similarity). Interacts with FCHSD2 (By similarity).

(Microbial infection) Interacts with E.coli effector protein EspF(U).

Family&Domains:

The WH1 (Wasp homology 1) domain may bind a Pro-rich ligand.

The CRIB (Cdc42/Rac-interactive-binding) region binds to the C-terminal WH2 domain in the autoinhibited state of the protein. Binding of Rho-type GTPases to the CRIB induces a conformation change and leads to activation.

Research Fields

· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Adherens junction. (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.

· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

· Human Diseases > Infectious diseases: Bacterial > Shigellosis.

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

· Human Diseases > Cancers: Overview > Choline metabolism in cancer. (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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WASP Antibody - #DF6160