EEF1A1 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from B16F10 cells, using EEF1A1 Antibody.

Product:	EEF1A1 Antibody
Catalog:	DF6156
Description:	Rabbit polyclonal antibody to EEF1A1
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.:	50kDa; 50kD(Calculated).
Uniprot:	P68104
RRID:	AB_2838123

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

EEF1A1 Antibody detects endogenous levels of total EEF1A1.

RRID:

AB_2838123
Cite Format: Affinity Biosciences Cat# DF6156, RRID:AB_2838123.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CCS 3; CCS3; Cervical cancer suppressor 3; chunp6927; CTCL tumor antigen; EE1A1; EEF 1; EEF1A; eEF1A-1; EEF1A1; EF-1-alpha-1; EF-Tu; EF1A; EF1a like protein; EF1A1_HUMAN; Elongation factor 1 alpha subunit; Elongation factor 1-alpha 1; Elongation factor Tu; Eukaryotic elongation factor 1 A-1; Eukaryotic translation elongation factor 1 alpha 1; Eukaryotic translation elongation factor 1 alpha 1 like 14; Glucocorticoid receptor AF 1 specific elongation factor; GRAF 1EF; HNGC:16303; ik:tdsubc_2a3; ik:tdsubc_2b3; LENG7; Leukocyte receptor cluster (LRC) member 7; Leukocyte receptor cluster member 7; Prostate tumor inducing protein 1; PTI1; tdsubc_2a3; Translation elongation factor 1 alpha 1 like 14; wu:fa91c07; wu:fa94b03; wu:fi13b09; xx:tdsubc_2a3; xx:tdsubc_2b3;

Immunogens

Immunogen:

Uniprot:

P68104(EF1A1_HUMAN) >>Visit HPA database.

Gene(ID):

EEF1A1(1915)

Expression:

P68104 EF1A1_HUMAN:

Brain, placenta, lung, liver, kidney, pancreas but barely detectable in heart and skeletal muscle.

Description:

Translation is the process where amino acid residues are assembled into polypeptides on ribosomes. This process is generally divided into three stages: initiation, elongation and termination. During elongation, mRNA and tRNA pair at the two active sites (A and P sites) on the ribosome. A number of eukaryotic elongation factors (eEFs) are involved in this process in mammalian cells (1). eEF1A, also called elongation factor Tu (EF-Tu), binds GTP and interacts with amino acyl-tRNAs to promote recruitment of amino acyl-tRNAs to the A-site of the ribosome (1). After GTP hydrolysis, GDP-eEF1A leaves the ribosome and is later converted back to the GTP-eEF1A by eEF1B (1). Studies have shown that eEF1A is phosphorylated under certain conditions, indicating that its activity is regulated at the post-translational level (2,3).

Sequence:

P68104 EF1A1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDISLWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPPYSQKRYEEIVKEVSTYIKKIGYNPDTVAFVPISGWNGDNMLEPSANMPWFKGWKVTRKDGNASGTTLLEALDCILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPVNVTTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDVRRGNVAGDSKNDPPMEAAGFTAQVIILNHPGQISAGYAPVLDCHTAHIACKFAELKEKIDRRSGKKLEDGPKFLKSGDAAIVDMVPGKPMCVESFSDYPPLGRFAVRDMRQTVAVGVIKAVDKKAAGAGKVTKSAQKAQKAK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Rabbit

100

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P68104 As Substrate

P68104

Site	PTM Type	Enzyme	Source
G2	Methylation		Uniprot
S21	Phosphorylation	P15056 (BRAF)	Uniprot
T22	Phosphorylation		Uniprot
T23	Phosphorylation		Uniprot
T24	Phosphorylation		Uniprot
Y29	Phosphorylation		Uniprot
K30	Acetylation		Uniprot
K30	Methylation		Uniprot
K30	Ubiquitination		Uniprot
K36	Acetylation		Uniprot
K36	Methylation		Uniprot
K36	Ubiquitination		Uniprot
R37	Methylation		Uniprot
K41	Acetylation		Uniprot
K41	Sumoylation		Uniprot
K41	Ubiquitination		Uniprot
K44	Acetylation		Uniprot
K44	Ubiquitination		Uniprot
K51	Methylation		Uniprot
K51	Ubiquitination		Uniprot
K55	Acetylation		Uniprot
K55	Methylation		Uniprot
K55	Sumoylation		Uniprot
K55	Ubiquitination		Uniprot
Y56	Phosphorylation		Uniprot
K62	Methylation		Uniprot
K62	Ubiquitination		Uniprot
S76	Phosphorylation		Uniprot
K79	Acetylation		Uniprot
K79	Methylation		Uniprot
T82	Phosphorylation		Uniprot
K84	Acetylation		Uniprot
K84	Methylation		Uniprot
K84	Ubiquitination		Uniprot
Y85	Phosphorylation		Uniprot
Y86	Phosphorylation		Uniprot
T88	Phosphorylation	P15056 (BRAF)	Uniprot
R96	Methylation		Uniprot
T104	Phosphorylation		Uniprot
T106	Phosphorylation		Uniprot
C111	S-Nitrosylation		Uniprot
S128	Phosphorylation		Uniprot
K129	Ubiquitination		Uniprot
T133	Phosphorylation		Uniprot
R134	Methylation		Uniprot
Y141	Phosphorylation		Uniprot
T142	Phosphorylation		Uniprot
K146	Acetylation		Uniprot
K146	Ubiquitination		Uniprot
K154	Methylation		Uniprot
K154	Ubiquitination		Uniprot
S157	Phosphorylation		Uniprot
T158	Phosphorylation		Uniprot
Y162	Phosphorylation		Uniprot
S163	Phosphorylation		Uniprot
K165	Methylation		Uniprot
K165	Ubiquitination		Uniprot
R166	Methylation		Uniprot
Y167	Phosphorylation		Uniprot
K172	Acetylation		Uniprot
K172	Methylation		Uniprot
K172	Ubiquitination		Uniprot
S175	Phosphorylation		Uniprot
T176	Phosphorylation		Uniprot
Y177	Phosphorylation		Uniprot
K179	Acetylation		Uniprot
K179	Methylation		Uniprot
K179	Ubiquitination		Uniprot
K180	Acetylation		Uniprot
K180	Sumoylation		Uniprot
K180	Ubiquitination		Uniprot
K212	Acetylation		Uniprot
K212	Sumoylation		Uniprot
K212	Ubiquitination		Uniprot
K215	Ubiquitination		Uniprot
K219	Ubiquitination		Uniprot
S224	Phosphorylation		Uniprot
C234	S-Nitrosylation		Uniprot
T239	Phosphorylation		Uniprot
T242	Phosphorylation		Uniprot
K244	Acetylation		Uniprot
K244	Ubiquitination		Uniprot
Y254	Phosphorylation		Uniprot
K255	Acetylation		Uniprot
K255	Methylation		Uniprot
K255	Ubiquitination		Uniprot
T261	Phosphorylation		Uniprot
R266	Methylation		Uniprot
T269	Phosphorylation		Uniprot
K273	Acetylation		Uniprot
K273	Ubiquitination		Uniprot
T286	Phosphorylation		Uniprot
K290	Ubiquitination		Uniprot
S291	Phosphorylation		Uniprot
S300	Phosphorylation	P36897 (TGFBR1)	Uniprot
K313	Sumoylation		Uniprot
K313	Ubiquitination		Uniprot
S316	Phosphorylation		Uniprot
K318	Acetylation		Uniprot
K318	Methylation		Uniprot
K318	Ubiquitination		Uniprot
S354	Phosphorylation		Uniprot
Y357	Phosphorylation		Uniprot
T365	Phosphorylation		Uniprot
S383	Phosphorylation		Uniprot
K385	Ubiquitination		Uniprot
K386	Acetylation		Uniprot
K386	Ubiquitination		Uniprot
K392	Acetylation		Uniprot
K392	Sumoylation		Uniprot
K392	Ubiquitination		Uniprot
K395	Acetylation		Uniprot
K395	Ubiquitination		Uniprot
S396	Phosphorylation		Uniprot
K408	Acetylation		Uniprot
K408	Methylation		Uniprot
K408	Ubiquitination		Uniprot
C411	S-Nitrosylation		Uniprot
S414	Phosphorylation		Uniprot
S416	Phosphorylation		Uniprot
Y418	Phosphorylation		Uniprot
R430	Methylation		Uniprot
T432	Phosphorylation	Q96RG2 (PASK) , O75116 (ROCK2)	Uniprot
K439	Acetylation		Uniprot
K439	Ubiquitination		Uniprot
K444	Ubiquitination		Uniprot
K450	Acetylation		Uniprot
K450	Ubiquitination		Uniprot
T452	Phosphorylation		Uniprot
K453	Acetylation		Uniprot
K453	Ubiquitination		Uniprot
S454	Phosphorylation		Uniprot
K457	Acetylation		Uniprot
K460	Acetylation		Uniprot

Research Backgrounds

P68104_EF1A1_HUMAN

Function:

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. With PARP1 and TXK, forms a complex that acts as a T helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production.

PTMs:

ISGylated.

Phosphorylated by TXK. Phosphorylation by PASK increases translation efficiency. Phosphorylated by ROCK2.

Trimethylated at Lys-79 by EEF1AKMT1. Methylated at Lys-165 by EEF1AKMT3, methylation by EEF1AKMT3 is dynamic as well as inducible by stress conditions, such as ER-stress, and plays a regulatory role on mRNA translation. Trimethylated at Lys-318 by EEF1AKMT2. Mono-, di-, and trimethylated at Lys-36 by EEF1AKMT4; trimethylated form is predominant. Methylation by EEF1AKMT4 contributes to the fine-tuning of translation rates for a subset of tRNAs. Trimethylated at Gly-2 by EEF1AKNMT. Mono- and dimethylated at Lys-55 by EEF1AKNMT; dimethylated form is predominant.

Subcellular Location:

Cytoplasm. Nucleus. Nucleus>Nucleolus. Cell membrane.
Note: Colocalizes with DLC1 at actin-rich regions in the cell periphery (PubMed:19158340). Translocates together with ZPR1 from the cytoplasm to the nucleus and nucleolus after treatment with mitogens (PubMed:8650580). Localization at the cell membrane depends on EEF1A1 phosphorylation status and the presence of PPP1R16B (PubMed:26497934).

Tissue Specificity:

Brain, placenta, lung, liver, kidney, pancreas but barely detectable in heart and skeletal muscle.

Subunit Structure:

Found in a nuclear export complex with XPO5, EEF1A1, Ran and aminoacylated tRNA. Interacts with PARP1, TXK, XPO5 and KARS1. May interact with ERGIC2. Interacts with IFIT1 (via TPR repeats 4-7) (By similarity). Interacts with DLC1, facilitating distribution to the membrane periphery and ruffles upon growth factor stimulation. Interacts with ZPR1; the interaction occurs in a epidermal growth factor (EGF)-dependent manner. Interacts with PPP1R16B. Interacts with SPHK1 and SPHK2; both interactions increase SPHK1 and SPHK2 kinase activity.

Family&Domains:

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.

Research Fields

· Genetic Information Processing > Translation > RNA transport.

· Human Diseases > Infectious diseases: Bacterial > Legionellosis.

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EEF1A1 Antibody - #DF6156