PRKAR1A Antibody - #DF6142

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Product: PRKAR1A Antibody
Catalog: DF6142
Description: Rabbit polyclonal antibody to PRKAR1A
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 43kDa; 43kD(Calculated).
Uniprot: P10644
RRID: AB_2838109

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
PRKAR1A Antibody detects endogenous levels of total PRKAR1A.
RRID:
AB_2838109
Cite Format: Affinity Biosciences Cat# DF6142, RRID:AB_2838109.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ACRDYS1; ADOHR; cAMP dependent protein kinase regulatory subunit alpha 1; cAMP dependent protein kinase regulatory subunit RIalpha; cAMP dependent protein kinase type I alpha regulatory chain; cAMP dependent protein kinase type I alpha regulatory subunit; cAMP-dependent protein kinase type I-alpha regulatory subunit; CAR; Carney complex type 1; CNC 1; CNC; CNC1; DKFZp779L0468; KAP0_HUMAN; MGC17251; PKA RIA; PKR 1; PKR1; PPNAD 1; PPNAD1; PRKAR 1; PRKAR1; PRKAR1A; PRKAR1A/RARA FUSION GENE; Protein kinase A type 1a regulatory subunit; Protein kinase cAMP dependent regulatory type I alpha; PTC2 CHIMERIC ONCOGENE, INCLUDED; Tissue specific extinguisher 1; Tissue-specific extinguisher 1; TSE 1; TSE1;

Immunogens

Immunogen:
Uniprot:

P10644(KAP0_HUMAN) >>Visit HPA database.

Gene(ID):
PRKAR1A(5573)
Expression:
P10644 KAP0_HUMAN:

Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.

Description:
The second messenger cyclic AMP (cAMP) activates cAMP-dependent protein kinase (PKA or cAPK) in mammalian cells and controls many cellular mechanisms such as gene transcription, ion transport, and protein phosphorylation (1). Inactive PKA is a heterotetramer composed of a regulatory subunit (R) dimer and a catalytic subunit (C) dimer. In this inactive state, the pseudosubstrate sequences on the R subunits block the active sites on the C subunits. Three C subunit isoforms (C-α, C-β, and C-γ) and two families of regulatory subunits (RI and RII) with distinct cAMP binding properties have been identified. The two R families exist in two isoforms, α and β (RI-α, RI-β, RII-α, and RII-β). Upon binding of cAMP to the R subunits, the autoinhibitory contact is eased and active monomeric C subunits are released. PKA shares substrate specificity with Akt (PKB) and PKC, which are characterized by an arginine at position -3 relative to the phosphorylated serine or threonine residue (2). Substrates that present this consensus sequence and have been shown to be phosphorylated by PKA are Bad (Ser155), CREB (Ser133), and GSK-3 (GSK-3α Ser21 and GSK-3β Ser9) (3-5). In addition, combined knock-down of PKA C-α and -β blocks cAMP-mediated phosphorylation of Raf (Ser43 and Ser259) (6). Autophosphorylation and phosphorylation by PDK-1 are two known mechanisms responsible for phosphorylation of the C subunit at Thr197 (7).
Sequence:
MESGSTAASEEARSLRECELYVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKEEAKQIQNLQKAGTRTDSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFSVSFIAGETVIQQGDEGDNFYVIDQGETDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQGEPGDEFFIILEGSAAVLQRRSENEEFVEVGRLGPSDYFGEIALLMNRPRAATVVARGPLKCVKLDRPRFERVLGPCSDILKRNIQQYNSFVSLSV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P10644 As Substrate

Site PTM Type Enzyme
M1 Acetylation
S5 Phosphorylation
S14 Phosphorylation
K24 Ubiquitination
K59 Ubiquitination
K63 Ubiquitination
K70 Ubiquitination
T73 Phosphorylation
T75 Phosphorylation
S77 Phosphorylation
S83 Phosphorylation Q00536 (CDK16) , P24941 (CDK2)
K92 Ubiquitination
S101 Phosphorylation
Y122 Phosphorylation
K123 Ubiquitination
K130 Acetylation
K130 Ubiquitination
K134 Ubiquitination
Y207 Phosphorylation
K222 Ubiquitination
S238 Phosphorylation
T239 Phosphorylation
K244 Ubiquitination
Y246 Phosphorylation
S251 Phosphorylation
K252 Ubiquitination
K261 Ubiquitination
Y323 Phosphorylation
T338 Phosphorylation
R342 Methylation
K346 Ubiquitination
K349 Ubiquitination
K367 Acetylation
K367 Ubiquitination
Y373 Phosphorylation
S375 Phosphorylation
S380 Phosphorylation

PTMs - P10644 As Enzyme

Substrate Site Source
P04439 (HLA-A) S336 Uniprot
P04439 (HLA-A) S337 Uniprot
P04439 (HLA-A) S343 Uniprot

Research Backgrounds

Function:

Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells.

PTMs:

The pseudophosphorylation site binds to the substrate-binding region of the catalytic chain, resulting in the inhibition of its activity.

Subcellular Location:

Cell membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.

Subunit Structure:

The inactive holoenzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP releases the two active catalytic monomers and the regulatory dimer. PRKAR1A also interacts with RFC2; the complex may be involved in cell survival. Interacts with AKAP4. Interacts with RARA; the interaction occurs in the presence of cAMP or FSH and regulates RARA transcriptional activity. Interacts with the phosphorylated form of PJA2. Interacts with CBFA2T3 (By similarity). Interacts with PRKX; regulates this cAMP-dependent protein kinase. Interacts with C2orf88/smAKAP; this interaction may target PRKAR1A to the plasma membrane. Interacts with AICDA.

Family&Domains:

Belongs to the cAMP-dependent kinase regulatory chain family.

Research Fields

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

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