ATG7 Antibody | Affinity Biosciences

WB
Cites

1/10

Western blot analysis of extracts from Caco-2, using ATG7 Antibody.

FIGURE 4|Effect of calycosin on autophagy in muscle of 5/6 Nx rats.

FIGURE 5|Effect of calycosin on the expression of CARM1 and H3R17me2a,and related proteins in rat muscles.

Fig.

S1 Fig.

Fig.

Product:	ATG7 Antibody
Catalog:	DF6130
Description:	Rabbit polyclonal antibody to ATG7
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	78kDa; 78kD(Calculated).
Uniprot:	O95352
RRID:	AB_2838097

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(90%), Bovine(100%), Horse(90%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(80%)

Clonality:

Polyclonal

Specificity:

ATG7 Antibody detects endogenous levels of total ATG7.

RRID:

AB_2838097
Cite Format: Affinity Biosciences Cat# DF6130, RRID:AB_2838097.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

1810013K23Rik; Apg 7; APG7 autophagy 7 like; APG7 autophagy 7-like (S. cerevisiae); APG7 like; APG7, S. cerevisiae, homolog of; APG7-like; APG7L; ATG 7; ATG12-activating enzyme E1 ATG7; ATG7; ATG7 autophagy related 7 homolog (S. cerevisiae); ATG7 autophagy related 7 homolog; ATG7_HUMAN; Atg7l; Autophagy 7, S. cerevisiae, homolog of; Autophagy related protein 7; Autophagy-related 7 (yeast); Autophagy-related protein 7; DKFZp434N0735; GSA 7; GSA7; hAGP7; Ubiquitin activating enzyme E1 like protein; Ubiquitin-activating enzyme E1-like protein; Ubiquitin-like modifier-activating enzyme ATG7;

Immunogens

Immunogen:

Uniprot:

O95352(ATG7_HUMAN) >>Visit HPA database.

Gene(ID):

ATG7(10533)

Expression:

O95352 ATG7_HUMAN:

Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.

Description:

Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10 (7,8).

Sequence:

O95352 ATG7_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAATGDPGLSKLQFAPFSSALDVGFWHELTQKKLNEYRLDEAPKDIKGYYYNGDSAGLPARLTLEFSAFDMSAPTPARCCPAIGTLYNTNTLESFKTADKKLLLEQAANEIWESIKSGTALENPVLLNKFLLLTFADLKKYHFYYWFCYPALCLPESLPLIQGPVGLDQRFSLKQIEALECAYDNLCQTEGVTALPYFLIKYDENMVLVSLLKHYSDFFQGQRTKITIGVYDPCNLAQYPGWPLRNFLVLAAHRWSSSFQSVEVVCFRDRTMQGARDVAHSIIFEVKLPEMAFSPDCPKAVGWEKNQKGGMGPRMVNLSECMDPKRLAESSVDLNLKLMCWRLVPTLDLDKVVSVKCLLLGAGTLGCNVARTLMGWGVRHITFVDNAKISYSNPVRQPLYEFEDCLGGGKPKALAAADRLQKIFPGVNARGFNMSIPMPGHPVNFSSVTLEQARRDVEQLEQLIESHDVVFLLMDTRESRWLPAVIAASKRKLVINAALGFDTFVVMRHGLKKPKQQGAGDLCPNHPVASADLLGSSLFANIPGYKLGCYFCNDVVAPGDSTRDRTLDQQCTVSRPGLAVIAGALAVELMVSVLQHPEGGYAIASSSDDRMNEPPTSLGLVPHQIRGFLSRFDNVLPVSLAFDKCTACSSKVLDQYEREGFNFLAKVFNSSHSFLEDLTGLTLLHQETQAAEIWDMSDDETI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Pig

Horse

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95352 As Substrate

O95352

Site	PTM Type	Source
A2	Acetylation	Uniprot
T5	Phosphorylation	Uniprot
S11	Phosphorylation	Uniprot
Y38	Phosphorylation	Uniprot
T120	Phosphorylation	Uniprot
K300	Ubiquitination	Uniprot
K306	Acetylation	Uniprot
K306	Ubiquitination	Uniprot
K352	Ubiquitination	Uniprot
K411	Ubiquitination	Uniprot
R420	Methylation	Uniprot
S490	Phosphorylation	Uniprot
S531	Phosphorylation	Uniprot
K652	Ubiquitination	Uniprot
S698	Phosphorylation	Uniprot

Research Backgrounds

O95352_ATG7_HUMAN

Function:

E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation. Plays a role in regulating the liver clock and glucose metabolism by mediating the autophagic degradation of CRY1 (clock repressor) in a time-dependent manner (By similarity).

PTMs:

Acetylated by EP300.

Subcellular Location:

Cytoplasm. Preautophagosomal structure.
Note: Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme.

Tissue Specificity:

Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.

Subunit Structure:

Homodimer. Interacts with ATG3 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. Forms intermediate conjugates with ATG8-like proteins such as GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A. Interacts with EP300 acetyltransferase. Interacts with FOXO1.

Family&Domains:

The C-terminal part of the protein is essential for the dimerization and interaction with ATG3 and ATG12.

The N-terminal FAP motif (residues 15 to 17) is essential for the formation of the ATG89-PE and ATG5-ATG12 conjugates.

Belongs to the ATG7 family.

Research Fields

· Cellular Processes > Transport and catabolism > Autophagy - other. (View pathway)

· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)

· Cellular Processes > Cell growth and death > Ferroptosis. (View pathway)

References

1). Integrin 51, as a Receptor of Fibronectin, Binds the FbaA Protein of Group A Streptococcus To Initiate Autophagy during Infection. mBio, 2020 (PubMed: 32518187) [IF=6.4]

2). Lupeol inhibits the proliferation and migration of MDA-MB-231 breast cancer cells via a novel crosstalk mechanism between autophagy and the EMT. Food & Function, 2022 (PubMed: 35448900) [IF=6.1]

3). Alginate oligosaccharides improve high-fat induced hepatic steatosis via PGC-1α-mediated lipophagy and fatty acid β-oxidation pathway. Journal of Functional Foods, 2023 [IF=5.6]

Application: WB Species: Mouse Sample: livers

Fig. 4. Effects of AOS on enhancing the lipophagy and free fatty acid (FFA) β-oxidation level in the NAFLD mouse livers. (A) the mRNA expression in the lipophagy and FFA β-oxidation pathways, (B) western blot results of lipophagy pathway, (C) the protein levels of lipophagy pathway, (D) western blot results of FFA β-oxidation pathway, (E) the protein levels of FFA β-oxidation pathway. The data are shown as the mean ± SEM. Values with different letters represent significant differences (p < 0.05)..

4). Respiratory Syncytial Virus Replication Is Promoted by Autophagy-Mediated Inhibition of Apoptosis. Journal of Virology, 2018 (PubMed: 29386287) [IF=5.4]

Application: WB Species: human Sample: Hep2 cells

Figure 5. |Inhibition of autophagy with specific shRNA targeting ATG5, ATG7 or BECN1 reduces RSV replication. (A-C) Hep2 cells stably expressing specific shRNA against ATG5 (Hep2-sh-ATG5), ATG7 (Hep2-sh-ATG7), BECN1 (Hep2-sh-BECN1) or luciferase (Hep2-sh-luciferase) were established as described in Materials and Methods. The silencing efficiency of ATG5 shRNA (A), ATG7 shRNA (B) and BECN1 shRNA (C) were validated by immunoblotting with anti-ATG5, anti-ATG7, anti-BECN1 and anti-GAPDH antibodies respectively.

5). Qingyihuaji Formula promotes apoptosis and autophagy through inhibition of MAPK/ERK and PI3K/Akt/mTOR signaling pathway on pancreatic cancer in vivo and in vitro. Journal of Ethnopharmacology, 2023 (PubMed: 36690307) [IF=5.4]

6). Calycosin inhibited autophagy and oxidative stress in chronic kidney disease skeletal muscle atrophy by regulating AMPK/SKP2/CARM1 signalling pathway. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2020 (PubMed: 32910538) [IF=5.3]

Application: IHC Species: rat Sample: muscles

FIGURE 4|Effect of calycosin on autophagy in muscle of 5/6 Nx rats. A, Representative images of autophagosomes observed in muscle of rat using TEM. B-E, Representative images and scores of LC3A/B and ATG7 expression in muscle of rats using IHC staining (×200). Bar = 50 µm. *P < 0.05 compared to the sham group. ##P < 0.01 compared to the 5/6 Nx model group

Application: WB Species: rat Sample: muscles

FIGURE 5|Effect of calycosin on the expression of CARM1 and H3R17me2a,and related proteins in rat muscles. A-B,Representative images and scores of the expression of CARM1 and H3R17me2a in muscle sections using IHC staining (×200).Bar = 50 µm. *P < 0.05 compared to the sham group. ##P < 0.01 compared to the 5/6 Nx model group. C-E, Representative immune blots of key proteins involved in muscle atrophy and proteins associated with the AMPK/SKP2/CARM1 signalling pathway

7). Pelargonic acid vanillylamide alleviates hepatic autophagy and ER stress in hepatic steatosis model. Food and Chemical Toxicology, 2023 (PubMed: 37611858) [IF=4.3]

8). HIF-1α enhances autophagy to alleviate apoptosis in marginal cells in the stria vascular in neonatal rats under hypoxia. The International Journal of Biochemistry & Cell Biology, 2022 (PubMed: 35779841) [IF=4.0]

9). Sporothrix globosa melanin regulates autophagy via the TLR2 signaling pathway in THP-1 macrophages. PLOS Neglected Tropical Diseases, 2022 (PubMed: 37141335) [IF=3.8]

Application: WB Species: Human Sample:

S1 Fig. Western blot analysis confirming Atg7 knockdown by siRNA. (A) Western blotting of Atg7 was conducted in THP-1 macrophages with or without knockdown treatment. (B) The ratio of Atg7/GAPDH was calculated. *P < 0.05, for the unpaired t-test. Representative images are displayed.

10). Abrogating PDK4 activates autophagy-dependent ferroptosis in breast cancer via ASK1/JNK pathway. Journal of cancer research and clinical oncology, 2024 (PubMed: 38678126) [IF=3.6]

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ATG7 Antibody - #DF6130