SMNDC1 Antibody - #DF6121

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Product: SMNDC1 Antibody
Catalog: DF6121
Description: Rabbit polyclonal antibody to SMNDC1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Bovine, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 27kDa; 27kD(Calculated).
Uniprot: O75940
RRID: AB_2838088

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Rabbit(91%), Dog(100%), Chicken(88%)
Clonality:
Polyclonal
Specificity:
SMNDC1 Antibody detects endogenous levels of total SMNDC1.
RRID:
AB_2838088
Cite Format: Affinity Biosciences Cat# DF6121, RRID:AB_2838088.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

30 kDa splicing factor SMNrp; MGC106917; MGC112663; SMN related protein; SMN-related protein; smndc1; SMNR; SPF30; SPF30_HUMAN; Splicing factor 30, survival of motor neuron-related; Survival motor neuron domain containing 1; Survival motor neuron domain containing protein 1; Survival motor neuron domain-containing protein 1; Survival of motor neuron related splicing factor 30; Survival of motor neuron-related-splicing factor 30;

Immunogens

Immunogen:
Uniprot:

O75940(SPF30_HUMAN) >>Visit HPA database.

Gene(ID):
SMNDC1(10285)
Expression:
O75940 SPF30_HUMAN:

Detected at intermediate levels in skeletal muscle, and at low levels in heart and pancreas.

Description:
SPF30 (survival of motor neuron-related-splicing factor 30), also known as SMNDC1 (survival motor neuron domain containing 1) or SMNR (SMN-related protein), is an essential splicing factor required for spliceosome assembly that belongs to the SMN family. It contains one Tudor domain with significant similarity to SMN (Survival Motor Neuron) and is expressed in skeletal muscle, pancreas and heart, localizing to Cajal bodies and nuclear speckles. SPF30 plays an important role in spliceosome assembly and directly interacts with five U snRNPs. The loss of SPF30 causes spliceosome assembly to arrest at prespliceosomes (A complex). This supports a function for SPF30 in mediating the incorporation/recruitment of U4/U5/U6 tri-snRNP to the prespliceosome. In addition, the overexpression of SPF30 can lead to apoptosis.
Sequence:
MSEDLAKQLASYKAQLQQVEAALSGNGENEDLLKLKKDLQEVIELTKDLLSTQPSETLASSDSFASTQPTHSWKVGDKCMAVWSEDGQCYEAEIEEIDEENGTAAITFAGYGNAEVTPLLNLKPVEEGRKAKEDSGNKPMSKKEMIAQQREYKKKKALKKAQRIKELEQEREDQKVKWQQFNNRAYSKNKKGQVKRSIFASPESVTGKVGVGTCGIADKPMTQYQDTSKYNVRHLMPQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
91
Chicken
88
Zebrafish
55
Horse
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75940 As Substrate

Site PTM Type Enzyme
K13 Ubiquitination
K37 Ubiquitination
S51 Phosphorylation
T52 Phosphorylation
T57 Phosphorylation
S63 Phosphorylation
S66 Phosphorylation
T67 Phosphorylation
T70 Phosphorylation
K165 Ubiquitination
R184 Methylation
S201 Phosphorylation
S204 Phosphorylation
T206 Phosphorylation
T213 Phosphorylation
K219 Acetylation
K219 Ubiquitination
T222 Phosphorylation
Y224 Phosphorylation
T227 Phosphorylation
K229 Ubiquitination

Research Backgrounds

Function:

Necessary for spliceosome assembly. Overexpression causes apoptosis.

Subcellular Location:

Nucleus speckle. Nucleus>Cajal body.
Note: Detected in nuclear speckles containing snRNP and in Cajal (coiled) bodies.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected at intermediate levels in skeletal muscle, and at low levels in heart and pancreas.

Subunit Structure:

Associates with spliceosomes. Associates with U4/U5/U6 tri-snRNP and with U2 snRNP.

Family&Domains:

The Tudor domain mediates association with dimethylarginines, which are common in snRNP proteins.

Belongs to the SMN family.

Research Fields

· Genetic Information Processing > Transcription > Spliceosome.

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