PSMG2 Antibody | Affinity Biosciences

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Western blot analysis of 293T lysates using PSMG2 antibody.

Product:	PSMG2 Antibody
Catalog:	DF6120
Description:	Rabbit polyclonal antibody to PSMG2
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Sheep, Rabbit, Dog
Mol.Wt.:	29kDa; 29kD(Calculated).
Uniprot:	Q969U7
RRID:	AB_2838087

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(88%), Sheep(88%), Rabbit(86%), Dog(88%)

Clonality:

Polyclonal

Specificity:

PSMG2 Antibody detects endogenous levels of total PSMG2.

RRID:

AB_2838087
Cite Format: Affinity Biosciences Cat# DF6120, RRID:AB_2838087.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CD40 ligand activated specific transcript 3; CLAST3; HCCA3; Hepatocellular carcinoma susceptibility protein; Hepatocellular carcinoma-susceptibility protein 3; HSPC260; HsT1707; Likely ortholog of mouse CD40 ligand activated specific transcript 3; MDS003; PAC-2; PAC2; proteasome (prosome, macropain) assembly chaperone 2; Proteasome assembly chaperone 2; psmG2; PSMG2_HUMAN; TNFSF5IP1; Tumor necrosis factor superfamily member 5-induced protein 1;

Immunogens

Immunogen:

Uniprot:

Q969U7(PSMG2_HUMAN) >>Visit HPA database.

Gene(ID):

PSMG2(56984)

Expression:

Q969U7 PSMG2_HUMAN:

Widely expressed with highest levels in lung, brain and colon. Moderately expressed in muscle, stomach, spleen and heart. Weakly expressed in small intestine, pancreas and liver. Highly expressed in hepatocellular carcinomas with low levels in surrounding liver tissue.

Description:

PSMG1 is a chaperone protein which promotes assembly of the 20S proteasome as part of a heterodimer with PSMG2. The PSMG1-PSMG2 heterodimer binds to the PSMA5 and PSMA7 proteasome subunits, promotes assembly of the proteasome alpha subunits into the heteroheptameric alpha ring and prevents alpha ring dimerization.

Sequence:

Q969U7 PSMG2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MFVPCGESAPDLAGFTLLMPAVSVGNVGQLAMDLIISTLNMSKIGYFYTDCLVPMVGNNPYATTEGNSTELSINAEVYSLPSRKLVALQLRSIFIKYKSKPFCEKLLSWVKSSGCARVIVLSSSHSYQRNDLQLRSTPFRYLLTPSMQKSVQNKIKSLNWEEMEKSRCIPEIDDSEFCIRIPGGGITKTLYDESCSKEIQMAVLLKFVSEGDNIPDALGLVEYLNEWLQILKPLSDDPTVSASRWKIPSSWRLLFGSGLPPALF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

Sheep

Dog

Rabbit

Zebrafish

Chicken

Xenopus

Horse

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q969U7 As Substrate

Q969U7

Site	PTM Type	Source
K111	Ubiquitination	Uniprot
T137	Phosphorylation	Uniprot
Y141	Phosphorylation	Uniprot
K149	Ubiquitination	Uniprot
K154	Ubiquitination	Uniprot
K156	Ubiquitination	Uniprot
K165	Ubiquitination	Uniprot
K188	Ubiquitination	Uniprot
K246	Ubiquitination	Uniprot

Research Backgrounds

Q969U7_PSMG2_HUMAN

Function:

Chaperone protein which promotes assembly of the 20S proteasome as part of a heterodimer with PSMG1. The PSMG1-PSMG2 heterodimer binds to the PSMA5 and PSMA7 proteasome subunits, promotes assembly of the proteasome alpha subunits into the heteroheptameric alpha ring and prevents alpha ring dimerization.

PTMs:

Degraded by the proteasome upon completion of 20S proteasome maturation.

Subcellular Location:

Nucleus.

Tissue Specificity:

Subunit Structure:

Forms a heterodimer with PSMG1. The PSMG1-PSMG2 heterodimer interacts directly with the PSMA5 and PSMA7 proteasome alpha subunits.

Family&Domains:

Belongs to the PSMG2 family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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PSMG2 Antibody - #DF6120