PEBP1 Antibody - #DF6110

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Product: PEBP1 Antibody
Catalog: DF6110
Description: Rabbit polyclonal antibody to PEBP1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 21kDa; 21kD(Calculated).
Uniprot: P30086
RRID: AB_2838078

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(90%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(90%), Dog(90%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
PEBP1 Antibody detects endogenous levels of total PEBP1.
RRID:
AB_2838078
Cite Format: Affinity Biosciences Cat# DF6110, RRID:AB_2838078.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Epididymis luminal protein 210; Epididymis secretory protein Li 34; Epididymis secretory protein Li 96; HCNP; HCNPpp; HEL 210; HEL S 34; HEL S 96; Hippocampal cholinergic neurostimulating peptide; Neuropolypeptide h3; PBP; PEBP; PEBP 1; PEBP-1; Pebp1; PEBP1_HUMAN; Phosphatidylethanolamine binding protein; Phosphatidylethanolamine binding protein 1; Prostatic binding protein; Prostatic-binding protein; R kip; Raf kinase inhibitor protein; Raf kinase inhibitory protein; RKIP;

Immunogens

Immunogen:
Uniprot:

P30086(PEBP1_HUMAN) >>Visit HPA database.

Gene(ID):
PEBP1(5037)
Description:
Raf kinase inhibitor protein (RKIP) is a member of the phosphatidylethanolamine-binding protein (PEBP) family that associates with Raf-1 and the MEK and MAP kinases (1). RKIP has been shown to complex with Raf-1, MEK, and ERK (2). Although MEK and ERK can simultaneously bind RKIP, the association between Raf-1 and RKIP and that of RKIP and MEK are mutually exclusive. Thus, RKIP competitively disrupts the Raf-1-MEK complex and effectively terminates signal transmission from Raf-1 to MAP kinases (2). The inhibitory effect of RKIP on MAP kinase signaling is eliminated by PKC phosphorylation of RKIP at Ser153 (3). PKC phosphorylation on Ser153 also promotes the association of RKIP with GRK2, which prevents GRK2-dependent internalization of GPCR (4). RKIP also interacts with modules of the NF-κB pathway, including NF-κB-inducing kinase (NIK), TAK1, IKKα and IKKβ (5). These interactions antagonize cytokine-induced activation of the NF-κB pathway (5). Restoration of RKIP expression is associated with the inhibition of prostate cancer metastasis, implying that RKIP may be a potential clinical target as a suppressor of tumor metastasis through inhibition of vascular invasion (6).
Sequence:
MPVDLSKWSGPLSLQEVDEQPQHPLHVTYAGAAVDELGKVLTPTQVKNRPTSISWDGLDSGKLYTLVLTDPDAPSRKDPKYREWHHFLVVNMKGNDISSGTVLSDYVGSGPPKGTGLHRYVWLVYEQDRPLKCDEPILSNRSGDHRGKFKVASFRKKYELRAPVAGTCYQAEWDDYVPKLYEQLSGK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Xenopus
100
Chicken
100
Dog
90
Zebrafish
90
Rabbit
90
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P30086 As Substrate

Site PTM Type Enzyme
S6 Phosphorylation
K7 Ubiquitination
S9 Phosphorylation
S13 Phosphorylation
T28 Phosphorylation
K39 Acetylation
T42 Phosphorylation
K47 Ubiquitination
T51 Phosphorylation
S52 Phosphorylation
S54 Phosphorylation
S60 Phosphorylation
K62 Acetylation
Y64 Phosphorylation
T69 Phosphorylation
S75 Phosphorylation
K77 Acetylation
K80 Acetylation
Y81 Phosphorylation
S98 Phosphorylation
S99 Phosphorylation
S104 Phosphorylation
Y106 Phosphorylation
S109 Phosphorylation
K113 Ubiquitination
Y120 Phosphorylation
Y125 Phosphorylation
R129 Methylation
K132 Acetylation
K132 Ubiquitination
S153 Phosphorylation P17252 (PRKCA) , Q05655 (PRKCD) , P05129 (PRKCG) , P05771 (PRKCB) , Q05513 (PRKCZ)
C168 S-Nitrosylation
Y169 Phosphorylation
Y176 Phosphorylation
K179 Acetylation
K179 Ubiquitination
Y181 Phosphorylation
S185 Phosphorylation
K187 Ubiquitination

Research Backgrounds

Function:

Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase (By similarity). Inhibits the kinase activity of RAF1 by inhibiting its activation and by dissociating the RAF1/MEK complex and acting as a competitive inhibitor of MEK phosphorylation.

HCNP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Seems to be mediated by a specific receptor (By similarity).

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Has a tendency to form dimers by disulfide cross-linking (By similarity). Interacts with RAF1 and this interaction is enhanced if RAF1 is phosphorylated on residues 'Ser-338', 'Ser-339', 'Tyr-340' and 'Tyr-341'. Interacts with ALOX15; in response to IL13/interleukin-13, prevents the interaction of PEBP1 with RAF1 to activate the ERK signaling cascade.

Family&Domains:

Belongs to the phosphatidylethanolamine-binding protein family.

References

1). Locostatin Alleviates Liver Fibrosis Induced by Carbon Tetrachloride in Mice. DIGESTIVE DISEASES AND SCIENCES, 2019 (PubMed: 30874989) [IF=3.1]

Application: WB    Species: mouse    Sample: liver

Fig. 2| Locostatin reduced liver α-SMA expression in mice treated with CCl4. a Representative α-SMA IHC liver stained sections. b *P < 0.05 compared to the control group;. #P < 0.01 compared to the CCl4 group. c *P < 0.05 compared to the control group;. #P < 0.05 compared to the CCl4 group

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