Grp75 Antibody - #DF6108

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Product: Grp75 Antibody
Catalog: DF6108
Description: Rabbit polyclonal antibody to Grp75
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Sheep, Rabbit
Mol.Wt.: 74kDa; 74kD(Calculated).
Uniprot: P38646
RRID: AB_2838076

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Sheep(100%), Rabbit(100%)
Clonality:
Polyclonal
Specificity:
Grp75 Antibody detects endogenous levels of total Grp75.
RRID:
AB_2838076
Cite Format: Affinity Biosciences Cat# DF6108, RRID:AB_2838076.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

75 kDa glucose regulated protein; 75 kDa glucose-regulated protein; CSA; Glucose Regulated Protein; Grp 75; GRP-75; GRP75; GRP75_HUMAN; Heat shock 70 kDa protein 9; Heat shock 70kD protein 9; heat shock 70kDa protein 9; Heat shock 70kDa protein 9B; Heat shock protein 74 kDa A; Heat shock protein A; Heat shock protein cognate 74; Hsc74; Hsp74; Hsp74a; HSPA9; Hspa9a; HSPA9B; MGC4500; mitochondrial; Mortalin 2; Mortalin; Mortalin perinuclear; Mortalin2; MOT 2; MOT; MOT2; Mthsp70; p66 mortalin; P66 MOT; PBP74; Peptide binding protein 74; Peptide-binding protein 74; Stress 70 protein mitochondrial; Stress 70 protein mitochondrial precursor; Stress-70 protein;

Immunogens

Immunogen:
Uniprot:

P38646(GRP75_HUMAN) >>Visit HPA database.

Gene(ID):
HSPA9(3313)
Description:
Grp75, also known as mortalin, is a member of Hsp70 family of chaperone proteins that is not heat-inducible (1,2). This protein is essential for transporting many mitochondrial proteins from the cytoplasm to mitochondria (3). Grp75 inactivates the tumor suppressor p53 (4). Studies found that Grp75 is overexpressed in many tumor tissues and immortalized human cell lines, suggesting its role in the tumor formation (5). Grp75 is also implicated in cell aging, as its overexpression appears to prolong the life span of human fibroblasts (6).
Sequence:
MISASRAAAARLVGAAASRGPTAARHQDSWNGLSHEAFRLVSRRDYASEAIKGAVVGIDLGTTNSCVAVMEGKQAKVLENAEGARTTPSVVAFTADGERLVGMPAKRQAVTNPNNTFYATKRLIGRRYDDPEVQKDIKNVPFKIVRASNGDAWVEAHGKLYSPSQIGAFVLMKMKETAENYLGHTAKNAVITVPAYFNDSQRQATKDAGQISGLNVLRVINEPTAAALAYGLDKSEDKVIAVYDLGGGTFDISILEIQKGVFEVKSTNGDTFLGGEDFDQALLRHIVKEFKRETGVDLTKDNMALQRVREAAEKAKCELSSSVQTDINLPYLTMDSSGPKHLNMKLTRAQFEGIVTDLIRRTIAPCQKAMQDAEVSKSDIGEVILVGGMTRMPKVQQTVQDLFGRAPSKAVNPDEAVAIGAAIQGGVLAGDVTDVLLLDVTPLSLGIETLGGVFTKLINRNTTIPTKKSQVFSTAADGQTQVEIKVCQGEREMAGDNKLLGQFTLIGIPPAPRGVPQIEVTFDIDANGIVHVSAKDKGTGREQQIVIQSSGGLSKDDIENMVKNAEKYAEEDRRKKERVEAVNMAEGIIHDTETKMEEFKDQLPADECNKLKEEISKMRELLARKDSETGENIRQAASSLQQASLKLFEMAYKKMASEREGSGSSGTGEQKEDQKEEKQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Rabbit
100
Pig
0
Horse
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P38646 As Substrate

Site PTM Type Enzyme
S3 Phosphorylation
S5 Phosphorylation
T22 Phosphorylation
S29 Phosphorylation
Y46 Phosphorylation
T62 Phosphorylation P33981 (TTK)
S65 Phosphorylation P33981 (TTK)
K76 Ubiquitination
T86 Phosphorylation
T87 Phosphorylation
K106 Ubiquitination
T111 Phosphorylation
Y118 Phosphorylation
T120 Phosphorylation
K121 Acetylation
K121 Ubiquitination
Y128 Phosphorylation
K135 Acetylation
K135 Ubiquitination
K138 Acetylation
K138 Ubiquitination
K143 Acetylation
K143 Ubiquitination
S148 Phosphorylation
K159 Acetylation
Y161 Phosphorylation
S162 Phosphorylation
S164 Phosphorylation
T177 Phosphorylation
Y181 Phosphorylation
T185 Phosphorylation
K187 Ubiquitination
T192 Phosphorylation
Y196 Phosphorylation
S200 Phosphorylation
T205 Phosphorylation
K206 Acetylation
K206 Ubiquitination
S212 Phosphorylation
R218 Methylation
Y230 Phosphorylation
K234 Acetylation
K234 Ubiquitination
S253 Phosphorylation
K265 Ubiquitination
S266 Phosphorylation
K288 Acetylation
K288 Ubiquitination
K291 Acetylation
K300 Acetylation
K300 Ubiquitination
R307 Methylation
T333 Phosphorylation
S336 Phosphorylation
K345 Acetylation
T356 Phosphorylation
R360 Methylation
T362 Phosphorylation
K368 Acetylation
K368 Ubiquitination
S376 Phosphorylation
K377 Acetylation
S378 Phosphorylation
T390 Phosphorylation
K394 Acetylation
K394 Ubiquitination
T398 Phosphorylation
R405 Methylation
S408 Phosphorylation
T462 Phosphorylation
T480 Phosphorylation
C487 S-Nitrosylation
K498 Acetylation
T504 Phosphorylation
R513 Methylation
S533 Phosphorylation
K555 Ubiquitination
K567 Acetylation
Y568 Phosphorylation
K595 Acetylation
K600 Acetylation
C608 S-Nitrosylation
K610 Acetylation
K612 Acetylation
K612 Ubiquitination
S616 Phosphorylation
R624 Methylation
S627 Phosphorylation
S638 Phosphorylation
S639 Phosphorylation
K646 Acetylation
K646 Ubiquitination
Y652 Phosphorylation
K653 Methylation
K653 Ubiquitination
S662 Phosphorylation
K671 Acetylation
K675 Acetylation
K678 Acetylation

Research Backgrounds

Function:

Chaperone protein which plays an important role in mitochondrial iron-sulfur cluster (ISC) biogenesis. Interacts with and stabilizes ISC cluster assembly proteins FXN, NFU1, NFS1 and ISCU. Regulates erythropoiesis via stabilization of ISC assembly. May play a role in the control of cell proliferation and cellular aging (By similarity).

Subcellular Location:

Mitochondrion. Nucleus>Nucleolus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts strongly with the intermediate form of FXN and weakly with its mature form. Interacts with HSCB. Associates with the mitochondrial contact site and cristae organizing system (MICOS) complex, composed of at least MICOS10/MIC10, CHCHD3/MIC19, CHCHD6/MIC25, APOOL/MIC27, IMMT/MIC60, APOO/MIC23/MIC26 and QIL1/MIC13. This complex was also known under the names MINOS or MitOS complex. The MICOS complex associates with mitochondrial outer membrane proteins SAMM50, MTX1, MTX2 and DNAJC11, mitochondrial inner membrane protein TMEM11 and with HSPA9. Interacts with DNLZ, the interaction is required to prevent self-aggregation. Interacts with TESPA1. Interacts with PDPN. Interacts with NFU1, NFS1 and ISCU.

Family&Domains:

Belongs to the heat shock protein 70 family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

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