FASN Antibody | Affinity Biosciences

Cites

1/7

Figure 3.

Fig.

FIGURE 2 | Knockdown of FGF9 in the liver of DIO mice exacerbated fatty liver phenotype.

Figure 3.

Figure 3 Effect of methyl brevifolincarboxylate (MBC) on expression of lipogenesis and lipid oxidation mRNA and proteins in OA-treated SK-HEP-1 cells (A), and primary murine hepatocytes (B).

FIG 1 (A) Cholesterol synthesis pathway.

FIGURE 8 Influence of SHBG treatment of lipid metabolism related modulators interplay in SAT (A–N).

Product:	FASN Antibody
Catalog:	DF6106
Description:	Rabbit polyclonal antibody to FASN
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Zebrafish, Bovine, Horse, Dog
Mol.Wt.:	272kDa; 273kD(Calculated).
Uniprot:	P49327
RRID:	AB_2811172

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Zebrafish(89%), Bovine(100%), Horse(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

FASN Antibody detects endogenous levels of total FASN.

RRID:

AB_2811172
Cite Format: Affinity Biosciences Cat# DF6106, RRID:AB_2811172.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

[Acyl-carrier-protein] S acetyltransferase; [Acyl-carrier-protein] S malonyltransferase; 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase; 3-oxoacyl-[acyl-carrier-protein] reductase; 3-oxoacyl-[acyl-carrier-protein] synthase; Enoyl-[acyl-carrier-protein] reductase; FAS; FAS_HUMAN; FASN; Fatty acid synthase; MGC14367; MGC15706; OA 519; Oleoyl-[acyl-carrier-protein] hydrolase; SDR27X1; Short chain dehydrogenase/reductase family 27X member 1;

Immunogens

Immunogen:

Uniprot:

P49327(FAS_HUMAN) >>Visit HPA database.

Gene(ID):

FASN(2194)

Expression:

P49327 FAS_HUMAN:

Ubiquitous. Prominent expression in brain, lung, and liver.

Description:

Fatty acid synthase (FASN) catalyzes the synthesis of long-chain fatty acids from acetyl-CoA and malonyl-CoA. FASN is active as a homodimer with seven different catalytic activities and produces lipids in the liver for export to metabolically active tissues or storage in adipose tissue. In most other human tissues, FASN is minimally expressed since they rely on circulating fatty acids for new structural lipid synthesis (1).Recently, increased expression of FASN has emerged as a phenotype common to most human carcinomas. In breast cancer, immunohistochemical staining showed that the levels of FASN are directly related to the size of breast tumors (2). Studies also showed that FASN is highly expressed in lung and prostate cancers and that FASN expression is an indicator of poor prognosis in breast and prostate cancer (3-5). Furthermore, inhibition of FASN is selectively cytotoxic to human cancer cells (5). Thus, increased interest has focused on FASN as a potential target for the diagnosis and treatment of cancer as well as metabolic syndrome (6,7).

Sequence:

P49327 FAS_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEEVVIAGMSGKLPESENLQEFWDNLIGGVDMVTDDDRRWKAGLYGLPRRSGKLKDLSRFDASFFGVHPKQAHTMDPQLRLLLEVTYEAIVDGGINPDSLRGTHTGVWVGVSGSETSEALSRDPETLVGYSMVGCQRAMMANRLSFFFDFRGPSIALDTACSSSLMALQNAYQAIHSGQCPAAIVGGINVLLKPNTSVQFLRLGMLSPEGTCKAFDTAGNGYCRSEGVVAVLLTKKSLARRVYATILNAGTNTDGFKEQGVTFPSGDIQEQLIRSLYQSAGVAPESFEYIEAHGTGTKVGDPQELNGITRALCATRQEPLLIGSTKSNMGHPEPASGLAALAKVLLSLEHGLWAPNLHFHSPNPEIPALLDGRLQVVDQPLPVRGGNVGINSFGFGGSNVHIILRPNTQPPPAPAPHATLPRLLRASGRTPEAVQKLLEQGLRHSQDLAFLSMLNDIAAVPATAMPFRGYAVLGGERGGPEVQQVPAGERPLWFICSGMGTQWRGMGLSLMRLDRFRDSILRSDEAVKPFGLKVSQLLLSTDESTFDDIVHSFVSLTAIQIGLIDLLSCMGLRPDGIVGHSLGEVACGYADGCLSQEEAVLAAYWRGQCIKEAHLPPGAMAAVGLSWEECKQRCPPGVVPACHNSKDTVTISGPQAPVFEFVEQLRKEGVFAKEVRTGGMAFHSYFMEAIAPPLLQELKKVIREPKPRSARWLSTSIPEAQWHSSLARTSSAEYNVNNLVSPVLFQEALWHVPEHAVVLEIAPHALLQAVLKRGLKPSCTIIPLMKKDHRDNLEFFLAGIGRLHLSGIDANPNALFPPVEFPAPRGTPLISPLIKWDHSLAWDVPAAEDFPNGSGSPSAAIYNIDTSSESPDHYLVDHTLDGRVLFPATGYLSIVWKTLARALGLGVEQLPVVFEDVVLHQATILPKTGTVSLEVRLLEASRAFEVSENGNLVVSGKVYQWDDPDPRLFDHPESPTPNPTEPLFLAQAEVYKELRLRGYDYGPHFQGILEASLEGDSGRLLWKDNWVSFMDTMLQMSILGSAKHGLYLPTRVTAIHIDPATHRQKLYTLQDKAQVADVVVSRWLRVTVAGGVHISGLHTESAPRRQQEQQVPILEKFCFTPHTEEGCLSERAALQEELQLCKGLVQALQTKVTQQGLKMVVPGLDGAQIPRDPSQQELPRLLSAACRLQLNGNLQLELAQVLAQERPKLPEDPLLSGLLDSPALKACLDTAVENMPSLKMKVVEVLAGHGHLYSRIPGLLSPHPLLQLSYTATDRHPQALEAAQAELQQHDVAQGQWDPADPAPSALGSADLLVCNCAVAALGDPASALSNMVAALREGGFLLLHTLLRGHPLGDIVAFLTSTEPQYGQGILSQDAWESLFSRVSLRLVGLKKSFYGSTLFLCRRPTPQDSPIFLPVDDTSFRWVESLKGILADEDSSRPVWLKAINCATSGVVGLVNCLRREPGGNRLRCVLLSNLSSTSHVPEVDPGSAELQKVLQGDLVMNVYRDGAWGAFRHFLLEEDKPEEPTAHAFVSTLTRGDLSSIRWVCSSLRHAQPTCPGAQLCTVYYASLNFRDIMLATGKLSPDAIPGKWTSQDSLLGMEFSGRDASGKRVMGLVPAKGLATSVLLSPDFLWDVPSNWTLEEAASVPVVYSTAYYALVVRGRVRPGETLLIHSGSGGVGQAAIAIALSLGCRVFTTVGSAEKRAYLQARFPQLDSTSFANSRDTSFEQHVLWHTGGKGVDLVLNSLAEEKLQASVRCLATHGRFLEIGKFDLSQNHPLGMAIFLKNVTFHGVLLDAFFNESSADWREVWALVQAGIRDGVVRPLKCTVFHGAQVEDAFRYMAQGKHIGKVVVQVLAEEPEAVLKGAKPKLMSAISKTFCPAHKSYIIAGGLGGFGLELAQWLIQRGVQKLVLTSRSGIRTGYQAKQVRRWRRQGVQVQVSTSNISSLEGARGLIAEAAQLGPVGGVFNLAVVLRDGLLENQTPEFFQDVCKPKYSGTLNLDRVTREACPELDYFVVFSSVSCGRGNAGQSNYGFANSAMERICEKRRHEGLPGLAVQWGAIGDVGILVETMSTNDTIVSGTLPQRMASCLEVLDLFLNQPHMVLSSFVLAEKAAAYRDRDSQRDLVEAVAHILGIRDLAAVNLDSSLADLGLDSLMSVEVRQTLERELNLVLSVREVRQLTLRKLQELSSKADEASELACPTPKEDGLAQQQTQLNLRSLLVNPEGPTLMRLNSVQSSERPLFLVHPIEGSTTVFHSLASRLSIPTYGLQCTRAAPLDSIHSLAAYYIDCIRQVQPEGPYRVAGYSYGACVAFEMCSQLQAQQSPAPTHNSLFLFDGSPTYVLAYTQSYRAKLTPGCEAEAETEAICFFVQQFTDMEHNRVLEALLPLKGLEERVAAAVDLIIKSHQGLDRQELSFAARSFYYKLRAAEQYTPKAKYHGNVMLLRAKTGGAYGEDLGADYNLSQVCDGKVSVHVIEGDHRTLLEGSGLESIISIIHSSLAEPRVSVREG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

100

Dog

100

Zebrafish

Chicken

Pig

Sheep

Xenopus

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P49327 As Substrate

P49327

Site	PTM Type	Source
M1	Acetylation	Uniprot
K12	Sumoylation	Uniprot
T34	Phosphorylation	Uniprot
K41	Ubiquitination	Uniprot
Y45	Phosphorylation	Uniprot
S63	Phosphorylation	Uniprot
K70	Acetylation	Uniprot
K70	Ubiquitination	Uniprot
S207	Phosphorylation	Uniprot
T211	Phosphorylation	Uniprot
C212	S-Nitrosylation	Uniprot
K213	Acetylation	Uniprot
K213	Ubiquitination	Uniprot
T217	Phosphorylation	Uniprot
Y222	Phosphorylation	Uniprot
S225	Phosphorylation	Uniprot
K235	Acetylation	Uniprot
K235	Ubiquitination	Uniprot
T245	Phosphorylation	Uniprot
T251	Phosphorylation	Uniprot
T253	Phosphorylation	Uniprot
K257	Acetylation	Uniprot
K257	Ubiquitination	Uniprot
S265	Phosphorylation	Uniprot
Y277	Phosphorylation	Uniprot
S279	Phosphorylation	Uniprot
S286	Phosphorylation	Uniprot
Y289	Phosphorylation	Uniprot
K298	Acetylation	Uniprot
K298	Ubiquitination	Uniprot
K326	Ubiquitination	Uniprot
S361	Phosphorylation	Uniprot
K436	Acetylation	Uniprot
K436	Ubiquitination	Uniprot
T463	Phosphorylation	Uniprot
Y470	Phosphorylation	Uniprot
S509	Phosphorylation	Uniprot
K528	Acetylation	Uniprot
K528	Ubiquitination	Uniprot
K611	Ubiquitination	Uniprot
K631	Acetylation	Uniprot
K631	Ubiquitination	Uniprot
C634	S-Nitrosylation	Uniprot
C642	S-Nitrosylation	Uniprot
K646	Acetylation	Uniprot
K646	Ubiquitination	Uniprot
K667	Ubiquitination	Uniprot
K673	Acetylation	Uniprot
K673	Ubiquitination	Uniprot
S724	Phosphorylation	Uniprot
S725	Phosphorylation	Uniprot
K772	Ubiquitination	Uniprot
K776	Acetylation	Uniprot
K776	Ubiquitination	Uniprot
K786	Acetylation	Uniprot
K787	Acetylation	Uniprot
K787	Ubiquitination	Uniprot
T827	Phosphorylation	Uniprot
S831	Phosphorylation	Uniprot
K897	Ubiquitination	Uniprot
T898	Phosphorylation	Uniprot
K927	Ubiquitination	Uniprot
T928	Phosphorylation	Uniprot
T930	Phosphorylation	Uniprot
S974	Phosphorylation	Uniprot
T976	Phosphorylation	Uniprot
T980	Phosphorylation	Uniprot
K992	Acetylation	Uniprot
K992	Ubiquitination	Uniprot
S1028	Phosphorylation	Uniprot
T1032	Phosphorylation	Uniprot
Y1047	Phosphorylation	Uniprot
K1065	Ubiquitination	Uniprot
Y1067	Phosphorylation	Uniprot
K1072	Acetylation	Uniprot
K1072	Ubiquitination	Uniprot
S1081	Phosphorylation	Uniprot
K1116	Acetylation	Uniprot
K1116	Ubiquitination	Uniprot
C1118	S-Nitrosylation	Uniprot
T1120	Phosphorylation	Uniprot
C1127	S-Nitrosylation	Uniprot
K1142	Ubiquitination	Uniprot
K1151	Acetylation	Uniprot
K1151	Ubiquitination	Uniprot
K1158	Ubiquitination	Uniprot
S1174	Phosphorylation	Uniprot
S1183	Phosphorylation	Uniprot
K1208	Ubiquitination	Uniprot
S1216	Phosphorylation	Uniprot
S1221	Phosphorylation	Uniprot
K1225	Ubiquitination	Uniprot
S1237	Phosphorylation	Uniprot
K1239	Acetylation	Uniprot
K1239	Ubiquitination	Uniprot
K1241	Ubiquitination	Uniprot
S1254	Phosphorylation	Uniprot
S1261	Phosphorylation	Uniprot
T1273	Phosphorylation	Uniprot
Y1367	Phosphorylation	Uniprot
S1373	Phosphorylation	Uniprot
S1379	Phosphorylation	Uniprot
S1382	Phosphorylation	Uniprot
K1393	Ubiquitination	Uniprot
T1407	Phosphorylation	Uniprot
S1411	Phosphorylation	Uniprot
S1421	Phosphorylation	Uniprot
K1429	Acetylation	Uniprot
K1429	Ubiquitination	Uniprot
K1444	Ubiquitination	Uniprot
C1471	S-Nitrosylation	Uniprot
S1479	Phosphorylation	Uniprot
Y1506	Phosphorylation	Uniprot
K1523	Acetylation	Uniprot
K1523	Ubiquitination	Uniprot
S1542	Phosphorylation	Uniprot
S1543	Phosphorylation	Uniprot
R1552	Methylation	Uniprot
K1582	Acetylation	Uniprot
K1582	Ubiquitination	Uniprot
S1584	Phosphorylation	Uniprot
K1591	Acetylation	Uniprot
K1591	Ubiquitination	Uniprot
T1593	Phosphorylation	Uniprot
S1594	Phosphorylation	Uniprot
S1597	Phosphorylation	Uniprot
S1701	Phosphorylation	Uniprot
K1704	Acetylation	Uniprot
K1704	Ubiquitination	Uniprot
S1723	Phosphorylation	Uniprot
K1739	Ubiquitination	Uniprot
S1747	Phosphorylation	Uniprot
K1752	Acetylation	Uniprot
K1752	Ubiquitination	Uniprot
K1771	Acetylation	Uniprot
K1827	Ubiquitination	Uniprot
K1847	Acetylation	Uniprot
K1847	Ubiquitination	Uniprot
K1851	Ubiquitination	Uniprot
K1866	Ubiquitination	Uniprot
K1869	Ubiquitination	Uniprot
K1871	Ubiquitination	Uniprot
S1874	Phosphorylation	Uniprot
S1877	Phosphorylation	Uniprot
K1878	Acetylation	Uniprot
K1878	Ubiquitination	Uniprot
K1911	Ubiquitination	Uniprot
K1927	Acetylation	Uniprot
K1927	Ubiquitination	Uniprot
S1947	Phosphorylation	Uniprot
S1948	Phosphorylation	Uniprot
K1993	Acetylation	Uniprot
K1993	Ubiquitination	Uniprot
K1995	Acetylation	Uniprot
K1995	Ubiquitination	Uniprot
S1997	Phosphorylation	Uniprot
T1999	Phosphorylation	Uniprot
R2004	Methylation	Uniprot
Y2034	Phosphorylation	Uniprot
R2043	Methylation	Uniprot
K2047	Ubiquitination	Uniprot
S2123	Phosphorylation	Uniprot
S2156	Phosphorylation	Uniprot
S2175	Phosphorylation	Uniprot
K2186	Ubiquitination	Uniprot
S2192	Phosphorylation	Uniprot
K2193	Ubiquitination	Uniprot
S2198	Phosphorylation	Uniprot
C2202	S-Nitrosylation	Uniprot
T2204	Phosphorylation	Uniprot
K2206	Acetylation	Uniprot
T2215	Phosphorylation	Uniprot
S2236	Phosphorylation	Uniprot
S2265	Phosphorylation	Uniprot
T2268	Phosphorylation	Uniprot
Y2269	Phosphorylation	Uniprot
T2356	Phosphorylation	Uniprot
K2391	Acetylation	Uniprot
K2391	Ubiquitination	Uniprot
K2406	Ubiquitination	Uniprot
R2413	Methylation	Uniprot
S2417	Phosphorylation	Uniprot
Y2424	Phosphorylation	Uniprot
Y2425	Phosphorylation	Uniprot
K2426	Acetylation	Uniprot
K2426	Ubiquitination	Uniprot
R2428	Methylation	Uniprot
Y2433	Phosphorylation	Uniprot
T2434	Phosphorylation	Uniprot
K2436	Acetylation	Uniprot
K2436	Ubiquitination	Uniprot
K2438	Acetylation	Uniprot
K2438	Ubiquitination	Uniprot
K2449	Sumoylation	Uniprot
K2449	Ubiquitination	Uniprot
K2471	Acetylation	Uniprot
K2471	Ubiquitination	Uniprot
T2483	Phosphorylation	Uniprot
S2488	Phosphorylation	Uniprot
S2499	Phosphorylation	Uniprot
S2507	Phosphorylation	Uniprot

Research Backgrounds

P49327_FAS_HUMAN

Function:

Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. This multifunctional protein has 7 catalytic activities as an acyl carrier protein.

Subcellular Location:

Cytoplasm. Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Tissue Specificity:

Ubiquitous. Prominent expression in brain, lung, and liver.

Subunit Structure:

Homodimer which is arranged in a head to tail fashion Ref.28). Interacts with CEACAM1; this interaction is insulin and phosphorylation-dependent; reduces fatty-acid synthase activity (By similarity).

Research Fields

· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)

· Metabolism > Lipid metabolism > Fatty acid biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Fatty acid metabolism.

· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)

References

1). β-patchoulene improves lipid metabolism to alleviate non-alcoholic fatty liver disease via activating AMPK signaling pathway. BIOMEDICINE & PHARMACOTHERAPY, 2021 (PubMed: 33341045) [IF=7.5]

Application: WB Species: Rat Sample: hepatic tissue

Fig. 4. β-PAE inhibits the expression of hepatic lipid synthesis-related proteins and genes in HFD-fed rats. (A–F) Western blot analysis on the expression of proteins referred to hepatic lipid synthesis including SREBP-1c, ACC1, p-ACC1, FASN, SCD1 and HMG-CR; (G–H) The mRNA expression of SREBP-1c and HMG-CR. Data are presented as the mean ± SD (n = 6~8). ##p < 0.01 vs. NC group; *p < 0.05, **p < 0.01 vs. Model group.

2). Sirtuin 3‐mediated deacetylation of acyl‐CoA synthetase family member 3 by protocatechuic acid attenuates nonalcoholic fatty liver disease. British Journal of Pharmacology, 2020 (PubMed: 32520409) [IF=7.3]

Application: WB Species: Mouse Sample: livers

Figure 3. PCA promotes fatty acid metabolism through SIRT3 activation. (A-E) Western blotting and real-time PCR analysis of hepatic FASN, SREBP-1c, CPT1, Acox1 and PPARα protein (n=5) and mRNA (n=6) levels in mouse livers. *P< 0.05 vs. the WT ND group, #P< 0.05 vs. the WT HFD group, &P< 0.05 vs. the SIRT3−/− ND group. (F-I) The protein expression of FASN, SREBP-1c, CPT1, Acox1, PPARα and SIRT3 was measured by Western blotting (n=5). The mRNA levels of (J) FASN, SREBP-1c (K) CPT1, Acox1 and PPARα were measured with real-time PCR (n=6). *P< 0.05 vs. the si-Control group, #P< 0.05 vs. the si-Control+PA group, &P< 0.05 vs. the si-Control+PA+PCA group.

3). FGF9 Alleviates the Fatty Liver Phenotype by Regulating Hepatic Lipid Metabolism. Frontiers in Pharmacology, 2022 (PubMed: 35517790) [IF=5.6]

Application: WB Species: mouse Sample: liver

FIGURE 2 | Knockdown of FGF9 in the liver of DIO mice exacerbated fatty liver phenotype.. (H) Representative Western blotting analysis of genes involved in lipid synthesis in mice as described in (A).

4). Peptides released from bovine α-lactalbumin by simulated digestion alleviated free fatty acids-induced lipid accumulation in HepG2 cells. Journal of Functional Foods, 2021 [IF=5.6]

5). Selegiline ameliorated dyslipidemia and hepatic steatosis in high-fat diet mice. International Immunopharmacology, 2023 (PubMed: 36822098) [IF=5.6]

6). Methyl Brevifolincarboxylate Attenuates Free Fatty Acid-Induced Lipid Metabolism and Inflammation in Hepatocytes through AMPK/NF-κB Signaling Pathway. International Journal of Molecular Sciences, 2021 (PubMed: 34576229) [IF=5.6]

Application: WB Species: Human Sample: SK-HEP-1 cells

Figure 3 Effect of methyl brevifolincarboxylate (MBC) on expression of lipogenesis and lipid oxidation mRNA and proteins in OA-treated SK-HEP-1 cells (A), and primary murine hepatocytes (B). Cells were treated with 0.5 mM of OA, and different concentrations of MBC (0, 20, 40, 60, and 80μM) for 48 h. Total RNA was isolated using a GENEzol reagent and mRNA was measured using qRT-PCR. Target gene mRNA levels were normalized to a reference gene β-actin. (C) Protein expression of FASN, ACC1, SREBP-1c, PPAR-α and (D) p-AMPK were detected by Western blot. All results are expressed as mean ± SD of three independent experiments. Data bars with similar letters were not significantly different (p ≤ 0.05).

7). Leptin Silencing Attenuates Lipid Accumulation through Sterol Regulatory Element-Binding Protein 1 Inhibition in Nasopharyngeal Carcinoma. International journal of molecular sciences, 2022 (PubMed: 35628510) [IF=5.6]

8). Augmentation of 3β-hydroxysteroid-Δ24 Reductase (DHCR24) Expression Induced by Bovine Viral Diarrhea Virus Infection Facilitates Viral Replication via Promoting Cholesterol Synthesis. Journal of Virology, 2022 (PubMed: 36468862) [IF=5.4]

Application: WB Species: bovine Sample: bovine cells

FIG 1 (A) Cholesterol synthesis pathway. (B) Heat map of the key genes (transcriptomic data) and corresponding proteins (proteomic data) involved in lipid metabolism in bovine viral diarrhea virus (BVDV)-infected bovine cells. (C, D) Identification of differentially expressed genes by quantitative reverse transcription-PCR (qRT-PCR) (C) and corresponding proteins by Western blotting (D). HMGCS1, 3- hydroxy-3-methylglutaryl-coenzyme A synthase 1; HMGCR, 3-hydroxy-3-methylglutaryl-coenzyme A reductase; MVK, mevalonate kinase; PMVK, phosphomevalonate kinase; MVD, diphosphomevalonate decarboxylase; FDPS, farnesyl diphosphate synthase; FDFT1, farnesyl diphosphate farnesyltransferase 1; SQLE, squalene epoxidase; GGPS, geranylgeranyl pyrophosphate synthase; LSS, lanosterol synthase; D8D7I, 3β-hydroxysterol-Δ8,7-isomerase; C5SD, 3β-hydroxysterol-C5 desaturase; DHCR7, 7-dehydrocholesterol reductase; DHCR24, 3β-hydroxysteroid-Δ24 reductase; SCD1, stearoyl-CoA desaturase; FASN, fatty acid synthase; ACACA, acetyl coenzyme A carboxylase alpha.

9). Sex hormone-binding globulin improves lipid metabolism and reduces inflammation in subcutaneous adipose tissue of metabolic syndrome-affected horses. Frontiers in molecular biosciences, 2023 (PubMed: 38146533) [IF=5.0]

Application: WB Species: Human Sample: adipose tissue

FIGURE 8 Influence of SHBG treatment of lipid metabolism related modulators interplay in SAT (A–N). The relative expression levels of LPL (A), SCD (B), PNPLA2 (C), PLIN1 (D), PPARA (E), FASN (F) in SAT were analyzed by RT-PCR. Protein levels of LPL (G), PLIN1 (H), FASN (I), SCD1 (J), ATGL (K, L) and HSL (M) were analyzed by Western blot ((N), representative membranes). Representative data are shown as mean ± SD.

10). microRNA-378b regulates ethanol-induced hepatic steatosis by targeting CaMKK2 to mediate lipid metabolism. Bioengineered, 2021 (PubMed: 34898362) [IF=4.9]

Application: WB Species: Human Sample: L-02 cells

Figure 3.miR-378b over-expression disturbs lipid metabolism in L-02 cells. (a) The TC level in L-02 cells. (b) The TG level in L-02 cells. (c) The expression level of miR-378b in L-02 cells. (d) mRNA expression levels of CaMKK2 in L-02 cells. (e) Western blot analysis for protein expression of CaMKK2 and p-AMPK/AMPK. (f) mRNA expression levels and protein expression levels for PPARα and CPT1. (g) mRNA expression levels and protein expression levels for FASN and SREBP1c. (h) Western blot analysis for protein expression of p-ACC/ACC. All data are expressed as the mean ± SD of at least three separate experiments. *p < 0.05, **p < 0.01 vs. control. #p < 0.05, ##p < 0.01 vs. miR-378b-mimics NC

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FASN Antibody - #DF6106