SOD1 Antibody | Affinity Biosciences

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Western blot analysis of extracts from Hela using SOD1 antibody.

DF6077 at 1/100 staining Mouse kidney tissue by IHC-P.

DF6077 at 1/100 staining Mouse brain tissue by IHC-P.

DF6077 at 1/100 staining Human esophageal cancer by IHC-P.

DF6077 at 1/100 staining Rat spleen tissue by IHC-P.

DF6077 at 1/100 staining Human colorectal cancer by IHC-P.

DF6077 at 1/100 staining Human mammary cancer by IHC-P.

DF6077 at 1/100 staining Human lung cancer by IHC-P.

DF6077 at 1/100 staining Rat testis tissue by IHC-P.

Product:	SOD1 Antibody
Catalog:	DF6077
Description:	Rabbit polyclonal antibody to SOD1
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	20kD; 16kD(Calculated).
Uniprot:	P00441
RRID:	AB_2838045

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(88%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(88%)

Clonality:

Polyclonal

Specificity:

SOD1 Antibody detects endogenous levels of total SOD1.

RRID:

AB_2838045
Cite Format: Affinity Biosciences Cat# DF6077, RRID:AB_2838045.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ALS; ALS1; Amyotrophic lateral sclerosis 1 adult; Cu/Zn SOD; Cu/Zn superoxide dismutase; Epididymis secretory protein Li 44; HEL S 44; Homodimer; hSod1; Indophenoloxidase A; IPOA; Mn superoxide dismutase; SOD; SOD soluble; SOD1; SOD2; SODC; SODC_HUMAN; Superoxide dismutase [Cu-Zn]; Superoxide dismutase 1; Superoxide dismutase 1 soluble; Superoxide dismutase Cu Zn; Superoxide dismutase cystolic;

Immunogens

Immunogen:

Uniprot:

P00441(SODC_HUMAN) >>Visit HPA database.

Gene(ID):

SOD1(6647)

Description:

SOD1, Cu/Zn superoxide dismutase, is a major antioxidant enzyme that catalyzes the conversion of superoxide anion to hydrogen peroxide and molecular oxygen (1). SOD1 is ubiquitously expressed and is localized in the cytosol, nucleus and mitochondrial intermembrane space. The SOD1 gene locus is on chromosome 21 in a region affected in Down Syndrome (2). In addition, over 100 distinct SOD1 inherited mutations have been identified in the familial form of amyotrophic lateral sclerosis (ALS), a progressive degenerative disease of motor neurons (3-5). Despite the fact that SOD1 helps to eliminate toxic reactive species, its mutations in ALS have been described as gain-of-function (5). The mechanism by which mutant SOD1 induces the neurodegeneration observed in ALS is still unclear. Mutant SOD1 proteins become misfolded and consequently oligomerize into high molecular weight species that aggregate and end up in proteinaceous inclusions (5).

Sequence:

P00441 SODC_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MATKAVCVLKGDGPVQGIINFEQKESNGPVKVWGSIKGLTEGLHGFHVHEFGDNTAGCTSAGPHFNPLSRKHGGPKDEERHVGDLGNVTADKDGVADVSIEDSVISLSGDHCIIGRTLVVHEKADDLGKGGNEESTKTGNAGSRLACGVIGIAQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

100

Sheep

100

Rabbit

100

Pig

Dog

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P00441 As Substrate

P00441

Site	PTM Type	Source
A2	Acetylation	Uniprot
T3	Phosphorylation	Uniprot
K4	Ubiquitination	Uniprot
C7	S-Nitrosylation	Uniprot
K10	Acetylation	Uniprot
K10	Ubiquitination	Uniprot
K24	Acetylation	Uniprot
K24	Ubiquitination	Uniprot
S26	Phosphorylation	Uniprot
K31	Ubiquitination	Uniprot
S35	Phosphorylation	Uniprot
K37	Ubiquitination	Uniprot
T40	Phosphorylation	Uniprot
T59	Phosphorylation	Uniprot
S60	Phosphorylation	Uniprot
S69	Phosphorylation	Uniprot
K71	Acetylation	Uniprot
K71	Ubiquitination	Uniprot
K76	Sumoylation	Uniprot
K76	Ubiquitination	Uniprot
R80	Methylation	Uniprot
T89	Phosphorylation	Uniprot
K92	Ubiquitination	Uniprot
S99	Phosphorylation	Uniprot
S103	Phosphorylation	Uniprot
S106	Phosphorylation	Uniprot
S108	Phosphorylation	Uniprot
K123	Acetylation	Uniprot
K123	Ubiquitination	Uniprot
K129	Ubiquitination	Uniprot
K137	Ubiquitination	Uniprot
R144	Methylation	Uniprot
C147	S-Nitrosylation	Uniprot

Research Backgrounds

P00441_SODC_HUMAN

Function:

Destroys radicals which are normally produced within the cells and which are toxic to biological systems.

PTMs:

Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation.

The ditryptophan cross-link at Trp-33 is responsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required.

Palmitoylation helps nuclear targeting and decreases catalytic activity.

Succinylation, adjacent to copper catalytic site, probably inhibits activity. Desuccinylation by SIRT5 enhances activity.

Subcellular Location:

Cytoplasm. Mitochondrion. Nucleus.
Note: Predominantly cytoplasmic; the pathogenic variants ALS1 Arg-86 and Ala-94 gradually aggregates and accumulates in mitochondria.

Subunit Structure:

Homodimer; non-disulfide linked. Homodimerization may take place via the ditryptophan cross-link at Trp-33. The pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 interact with RNF19A, whereas wild-type protein does not. The pathogenic variants ALS1 Arg-86 and Ala-94 interact with MARCH5, whereas wild-type protein does not.

Family&Domains:

Belongs to the Cu-Zn superoxide dismutase family.

Research Fields

· Cellular Processes > Transport and catabolism > Peroxisome. (View pathway)

· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Human Diseases > Neurodegenerative diseases > Prion diseases.

· Organismal Systems > Aging > Longevity regulating pathway - multiple species. (View pathway)

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For Research Use Only.
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SOD1 Antibody - #DF6077