NAMPT Antibody - #DF6059

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Product: NAMPT Antibody
Catalog: DF6059
Description: Rabbit polyclonal antibody to NAMPT
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 52kDa; 56kD(Calculated).
Uniprot: P43490
RRID: AB_2838028

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(94%), Bovine(94%), Horse(88%), Sheep(94%), Rabbit(88%), Dog(88%)
Clonality:
Polyclonal
Specificity:
NAMPT Antibody detects endogenous levels of total NAMPT.
RRID:
AB_2838028
Cite Format: Affinity Biosciences Cat# DF6059, RRID:AB_2838028.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

1110035O14Rik; AI314458; AI480535; DKFZP666B131; EC 2.4.2.12; MGC117256; NAmPRTase; Nampt; NAMPT_HUMAN; Nicotinamide phosphoribosyltransferase; PBEF 1; PBEF; PBEF1; Pre B cell colony enhancing factor 1; Pre B cell colony enhancing factor; Pre B cell enhancing factor; Pre-B cell-enhancing factor; Pre-B-cell colony-enhancing factor 1; VF; Visfatin;

Immunogens

Immunogen:
Uniprot:

P43490(NAMPT_HUMAN) >>Visit HPA database.

Gene(ID):
NAMPT(10135)
Expression:
P43490 NAMPT_HUMAN:

Expressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues.

Description:
Nicotinamide phosphoribosyltransferase(NAMPT) has two usual synonyms termed Visfatin and PBEF. Its primary role is to catalyze the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD, which is the rate limiting component in the mammalian NAD biosynthesis pathway. NAMPT is localized in cytoplasm and expressed in large amounts in bone marrow, liver tissue, and muscle tissues. NAMPT inhibits neutrophil apoptosis in experimental inflammation and clinical sepsis. NAMPT levels are altered in plasma of patients with type 2 diabetes mellitus (T2DM), and it is now evidenced that NAMPT may plays a role in lipid metabolism.
Sequence:
MNPAAEAEFNILLATDSYKVTHYKQYPPNTSKVYSYFECREKKTENSKLRKVKYEETVFYGLQYILNKYLKGKVVTKEKIQEAKDVYKEHFQDDVFNEKGWNYILEKYDGHLPIEIKAVPEGFVIPRGNVLFTVENTDPECYWLTNWIETILVQSWYPITVATNSREQKKILAKYLLETSGNLDGLEYKLHDFGYRGVSSQETAGIGASAHLVNFKGTDTVAGLALIKKYYGTKDPVPGYSVPAAEHSTITAWGKDHEKDAFEHIVTQFSSVPVSVVSDSYDIYNACEKIWGEDLRHLIVSRSTQAPLIIRPDSGNPLDTVLKVLEILGKKFPVTENSKGYKLLPPYLRVIQGDGVDINTLQEIVEGMKQKMWSIENIAFGSGGGLLQKLTRDLLNCSFKCSYVVTNGLGINVFKDPVADPNKRSKKGRLSLHRTPAGNFVTLEEGKGDLEEYGQDLLHTVFKNGKVTKSYSFDEIRKNAQLNIELEAAHH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
94
Bovine
94
Sheep
94
Horse
88
Dog
88
Rabbit
88
Xenopus
56
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P43490 As Substrate

Site PTM Type Enzyme
M1 Acetylation
T15 Phosphorylation
S17 Phosphorylation
Y18 Phosphorylation
K24 Ubiquitination
Y26 Phosphorylation
Y34 Phosphorylation
Y36 Phosphorylation
K48 Ubiquitination
Y54 Phosphorylation
T57 Phosphorylation
Y60 Phosphorylation
Y64 Phosphorylation
Y69 Phosphorylation
K84 Ubiquitination
Y87 Phosphorylation
K88 Ubiquitination
K99 Ubiquitination
K107 Ubiquitination
K117 Ubiquitination
K174 Ubiquitination
Y175 Phosphorylation
Y188 Phosphorylation
K189 Ubiquitination
R196 Methylation
K228 Ubiquitination
K229 Ubiquitination
K234 Ubiquitination
S241 Phosphorylation
K255 Ubiquitination
K259 Ubiquitination
S314 Phosphorylation
K330 Ubiquitination
K331 Ubiquitination
T335 Phosphorylation
S338 Phosphorylation
K339 Ubiquitination
K342 Ubiquitination
K369 Acetylation
K369 Ubiquitination
K371 Ubiquitination
S374 Phosphorylation
S382 Phosphorylation
K389 Ubiquitination
S398 Phosphorylation
Y403 Phosphorylation
K415 Acetylation
K423 Acetylation
K423 Ubiquitination
S431 Phosphorylation
T435 Phosphorylation
K447 Ubiquitination
Y453 Phosphorylation
K469 Ubiquitination
S470 Phosphorylation
S472 Phosphorylation

Research Backgrounds

Function:

Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-ARNTL/BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression (By similarity).

Subcellular Location:

Nucleus. Cytoplasm. Secreted.
Note: Under non-inflammatory conditions, visfatin predominantly exhibits a granular pattern within the nucleus. Secreted by endothelial cells upon IL-1beta stimulation. Abundantly secreted in milk, reaching 100-fold higher concentrations compared to maternal serum.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the NAPRTase family.

Research Fields

· Metabolism > Metabolism of cofactors and vitamins > Nicotinate and nicotinamide metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

References

1). Metabolomics Analysis Reveals Therapeutic Effects of α-Mangostin on Collagen-Induced Arthritis in Rats by Down-regulating Nicotinamide Phosphoribosyltransferase. INFLAMMATION, 2019 (PubMed: 30484004) [IF=5.1]

Application: WB    Species: rat    Sample: HFLS-RA cells

Fig. 5.| Effects of MAN on the levels of NAMPT and NAD in HFLS-RA cells in vitro. a Survival rates of cells investigated by MTT assay; b MAN at 12 μg/ml elicited intracellular ROS accumulation in the time-dependent manner; c the relative levels of intracellular NAD+/NADH; d expression of iNAMPT in HFLS-RA cells; e quantitative result of d; f secretion of eNAMPT by HFLS-RA cells; g expression of iNAMPT under the co-treatment with NAD and MAN; h secretion of eNAMPT under the co-treatments with NAD/NMN and MAN. Statistical significance: c–f, *p < 0.05 and **p < 0.01 compared with untreated cells; h *p < 0.05 and **p < 0.01 compared with LPS-treated cells.

Application: IHC    Species: rat    Sample: synovium

Fig. 4. |Effects of MAN treatments on the levels of NAMPT and NAD in vivo. a NAMPT expression in synovium: a, normal; b, CIA model; c MAN treated;b quantitative result of A; c levels of NAD+ in serum from rats receiving MAN treatment. Statistical significance: b *p < 0.05 compared with CIA models; c *p < 0.05 and **p < 0.01 compared with untreated rats.
2). Bioactive fractions from Securidaca inappendiculata alleviated collagen-induced arthritis in rats by regulating metabolism-related signaling. KAOHSIUNG JOURNAL OF MEDICAL SCIENCES, 2020 (PubMed: 32187848) [IF=3.3]

Application: IHC    Species: rat    Sample: fat pad

FIGURE 6 |Effects of xanthone-rich fraction (XRF) and xanthone-deprived fraction (XDF) on protein expressions in rats. A, Nicotinamide phosphoribosyl transferase (NAMPT) and Sirt1 expressions in fat pad
3). Role of Nampt overexpression in a rat model of Hashimoto's thyroiditis and its mechanism of action. Experimental and Therapeutic Medicine, 2020 (PubMed: 32256774) [IF=2.7]

Application: WB    Species: rat    Sample: thyroid

Figure 3. |Effect of Nampt on TLR4 expression in HT rats. (A) Expression of Nampt and TLR4 mRNA in rat thyroid tissues. (B) Expression of Nampt and TLR4 protein in rat thyroid tissues. *P<0.05 compared with the control group; #P<0.01 compared with the model+NC group.

Application: IHC    Species: rat    Sample: thyroid

Figure 2. |Immunohistochemical staining of Nampt in rat thyroid tissues (magnification, x200) in the Control and Model groups. Arrowhead, Nampt‑positive. Nampt, nicotinamide phosphoribosyltransferase.
4). α-Mangostin Alleviated Lipopolysaccharide Induced Acute Lung Injury in Rats by Suppressing NAMPT/NAD Controlled Inflammatory Reactions. Evidence-based Complementary and Alternative Medicine, 2018 (PubMed: 30405740)

Application: WB    Species: rat    Sample: RAW 264.7 cells

Figure 3: | Regulation of MAN on NAMPT and Sirt1 in RAW 264.7 cells in vitro. (a) Expression of iNAMPT in cells; (b) levels of eNAMPT in culture medium; (c) expression of Sirt1 in cells; (d) deacetylation capability of Sirt1 indicated by expression of ace-p65 in cells. Statistical signifcance: ∗p < 0.05 and ∗∗p < 0.01 compared with LPS treated cells.

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