Cytokeratin 20 Antibody | Affinity Biosciences

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Western blot analysis of extracts from Hela using KRT20 Antibody.

Product:	Cytokeratin 20 Antibody
Catalog:	DF6051
Description:	Rabbit polyclonal antibody to Cytokeratin 20
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Bovine, Horse, Sheep, Chicken, Xenopus
Mol.Wt.:	48kDa; 48kD(Calculated).
Uniprot:	P35900
RRID:	AB_2838021

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Bovine(100%), Horse(100%), Sheep(100%), Chicken(100%), Xenopus(86%)

Clonality:

Polyclonal

Specificity:

Cytokeratin 20 Antibody detects endogenous levels of total Cytokeratin 20.

RRID:

AB_2838021
Cite Format: Affinity Biosciences Cat# DF6051, RRID:AB_2838021.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CD20; CK 20; CK-20; CK20; Cytokeratin-20; Cytokeratin20; K1C20_HUMAN; K20; KA20; Keratin 20; keratin 20, type I; keratin 21, rat, homolog of; Keratin; Keratin type I cytoskeletal 20; Keratin-20; Keratin20; KRT 20; KRT 21; KRT20; KRT21; MGC35423; OTTHUMP00000164518; Protein IT; type I cytoskeletal 20;

Immunogens

Immunogen:

Uniprot:

P35900(K1C20_HUMAN) >>Visit HPA database.

Gene(ID):

KRT20(54474)

Expression:

P35900 K1C20_HUMAN:

Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa.

Description:

Intermediate-sized filament (IF) protein designated cytokeratin (CK) 20 is a major cellular protein of mature enterocytes and goblet cells commonly found in mucosal epithelium of the mammalian gastrointestinal tract (1). Results strongly suggest that transcriptional regulation of keratin genes in the intestinal epithelium occurs at the level of both immature and terminally differentiated epithelial cells, and is tightly regulated during both fetal development and crypt-to-villus differentiation of the intestinal epithelium (2). CK20 has recently been reported to be useful to distinguish between primary and metastatic lung adenocarcinoma. CK20 expression was significantly more prevalent in adenocarcinoma that originated in the GI tract than that of pulmonary or breast origin (3).

Sequence:

P35900 K1C20_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDFSRRSFHRSLSSSLQAPVVSTVGMQRLGTTPSVYGGAGGRGIRISNSRHTVNYGSDLTGGGDLFVGNEKMAMQNLNDRLASYLEKVRTLEQSNSKLEVQIKQWYETNAPRAGRDYSAYYRQIEELRSQIKDAQLQNARCVLQIDNAKLAAEDFRLKYETERGIRLTVEADLQGLNKVFDDLTLHKTDLEIQIEELNKDLALLKKEHQEEVDGLHKHLGNTVNVEVDAAPGLNLGVIMNEMRQKYEVMAQKNLQEAKEQFERQTAVLQQQVTVNTEELKGTEVQLTELRRTSQSLEIELQSHLSMKESLEHTLEETKARYSSQLANLQSLLSSLEAQLMQIRSNMERQNNEYHILLDIKTRLEQEIATYRRLLEGEDVKTTEYQLSTLEERDIKKTRKIKTVVQEVVDGKVVSSEVKEVEENI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

100

Sheep

100

Chicken

100

Xenopus

Rabbit

Pig

Dog

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P35900 As Substrate

P35900

Site	PTM Type	Enzyme	Source
S11	Phosphorylation		Uniprot
S13	Phosphorylation	P49137 (MAPKAPK2) , P17252 (PRKCA)	Uniprot
S34	Phosphorylation		Uniprot
Y36	Phosphorylation		Uniprot
R42	Methylation		Uniprot
Y84	Phosphorylation		Uniprot
S94	Phosphorylation		Uniprot
S118	Phosphorylation		Uniprot
Y120	Phosphorylation		Uniprot
Y121	Phosphorylation		Uniprot
K149	Ubiquitination		Uniprot
T168	Phosphorylation		Uniprot
T369	Phosphorylation		Uniprot
Y370	Phosphorylation		Uniprot
Y384	Phosphorylation		Uniprot
S387	Phosphorylation		Uniprot
T402	Phosphorylation		Uniprot

Research Backgrounds

P35900_K1C20_HUMAN

Function:

Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine (By similarity).

PTMs:

Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury (By similarity).

Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228.

Subcellular Location:

Cytoplasm.

Tissue Specificity:

Subunit Structure:

Heterotetramer of two type I and two type II keratins. Associates with KRT8.

Family&Domains:

Belongs to the intermediate filament family.

Research Fields

· Organismal Systems > Endocrine system > Estrogen signaling pathway. (View pathway)

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Cytokeratin 20 Antibody - #DF6051