APG5L/ATG5 Antibody - #DF6010

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Product: APG5L/ATG5 Antibody
Catalog: DF6010
Description: Rabbit polyclonal antibody to APG5L/ATG5
Application: WB IHC
Cited expt.: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 35kD,56kD(complex); 32kD(Calculated).
Uniprot: Q9H1Y0
RRID: AB_2837987

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MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(93%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(93%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
APG5L/ATG5 Antibody detects endogenous levels of total APG5L/ATG5.
RRID:
AB_2837987
Cite Format: Affinity Biosciences Cat# DF6010, RRID:AB_2837987.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

APG 5; APG 5L; APG5; APG5 autophagy 5 like; APG5 like; APG5-like; APG5L; Apoptosis specific protein; Apoptosis-specific protein; ASP; ATG 5; Atg5; ATG5 autophagy related 5 homolog; ATG5_HUMAN; Autophagy protein 5; Autophagy related 5; hAPG5; Homolog of S Cerevisiae autophagy 5; OTTHUMP00000040507;

Immunogens

Immunogen:

A synthesized peptide derived from human APG5L/ATG5, corresponding to a region within C-terminal amino acids.

Uniprot:

Q9H1Y0(ATG5_HUMAN) >>Visit HPA database.

Gene(ID):
ATG5(9474)
Expression:
Q9H1Y0 ATG5_HUMAN:

Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells.

Description:
Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1,2). Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (3). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles (4-6). This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10 (7,8).
Sequence:
MTDDKDVLRDVWFGRIPTCFTLYQDEITEREAEPYYLLLPRVSYLTLVTDKVKKHFQKVMRQEDISEIWFEYEGTPLKWHYPIGLLFDLLASSSALPWNITVHFKSFPEKDLLHCPSKDAIEAHFMSCMKEADALKHKSQVINEMQKKDHKQLWMGLQNDRFDQFWAINRKLMEYPAEENGFRYIPFRIYQTTTERPFIQKLFRPVAADGQLHTLGDLLKEVCPSAIDPEDGEKKNQVMIHGIEPMLETPLQWLSEHLSYPDNFLHISIIPQPTD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Zebrafish
93
Chicken
93
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Involved in autophagic vesicle formation. Conjugation with ATG12, through a ubiquitin-like conjugating system involving ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. Involved in mitochondrial quality control after oxidative damage, and in subsequent cellular longevity. Plays a critical role in multiple aspects of lymphocyte development and is essential for both B and T lymphocyte survival and proliferation. Required for optimal processing and presentation of antigens for MHC II. Involved in the maintenance of axon morphology and membrane structures, as well as in normal adipocyte differentiation. Promotes primary ciliogenesis through removal of OFD1 from centriolar satellites and degradation of IFT20 via the autophagic pathway.

May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Plays a crucial role in IFN-gamma-induced autophagic cell death by interacting with FADD.

(Microbial infection) May act as a proviral factor. In association with ATG12, negatively regulates the innate antiviral immune response by impairing the type I IFN production pathway upon vesicular stomatitis virus (VSV) infection. Required for the translation of incoming hepatitis C virus (HCV) RNA and, thereby, for initiation of HCV replication, but not required once infection is established.

PTMs:

Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane.

Acetylated by EP300.

Subcellular Location:

Cytoplasm. Preautophagosomal structure membrane>Peripheral membrane protein.
Note: Colocalizes with nonmuscle actin. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed (By similarity). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells.

Family&Domains:

Belongs to the ATG5 family.

Research Fields

· Cellular Processes > Transport and catabolism > Autophagy - other.   (View pathway)

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Cellular Processes > Cell growth and death > Ferroptosis.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Shigellosis.

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway - multiple species.   (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > RIG-I-like receptor signaling pathway.   (View pathway)

References

1). Microbiota-derived H2S induces c-kit+ cDC1 autophagic cell death and liver inflammation in metabolic dysfunction-associated steatohepatitis. Nature communications, 2025 (PubMed: 40044736) [IF=16.6]
2). Rationally designed catalytic nanoplatform for enhanced chemoimmunotherapy via deploying endogenous plus exogenous copper and remodeling tumor microenvironment. Journal of nanobiotechnology, 2024 (PubMed: 39252079) [IF=10.2]
3). Genetically-engineered Salmonella typhimurium expressing FGF21 promotes neurological recovery in ischemic stroke via FGFR1/AMPK/mTOR pathway. Journal of neuroinflammation, 2025 (PubMed: 40581646) [IF=9.3]

Application: WB    Species: Rat    Sample: PC-12 cells

Fig. 7 S.t-ΔpGFGF21(+) enhances autophagy through binding to the FGFR1 receptor to activate the AMPK-mTOR pathway. (A-B) Immunofluorescence staining and quantitative analyses of p-FGFR1 (green) in PC-12 cells, n = 5, scale bar = 100 μm; (C-D) Immunofluorescence staining and quantitative analyses of p-FGFR1 (green) in primary neuron cells, n = 5, scale bar = 100 μm; (E-F) Representative images of p-FGFR1 (green) and NeuN (red) staining on day 5 in the cerebral cortex, n = 6, scale bar = 100 μm. (G-J) Expression level and relative quantitative analysis of FGFR1, p-FGFR1, AMPK, p-AMPK, and p-mTOR in the cerebral cortex on day 5 post stroke, n = 6; (K-N) Representative western blot images and relative quantification analysis of autophagy relative protein including p62, ATG5, and LC 3 in the cerebral cortex of each group, n = 6. * p 
4). Morusin, a novel inhibitor of ACLY, induces mitochondrial apoptosis in hepatocellular carcinoma cells through ROS-mediated mitophagy. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2024 (PubMed: 39341077) [IF=6.9]
5). d-Borneol enhances cisplatin sensitivity via autophagy dependent EMT signaling and NCOA4-mediated ferritinophagy. PHYTOMEDICINE, 2022 (PubMed: 36030746) [IF=6.7]
6). Effect of mitophagy in the formation of osteomorphs derived from osteoclasts. iScience, 2023 (PubMed: 37250312) [IF=5.8]
7). Moringa oleifera leaves protein suppresses T-lymphoblastic leukemogenesis via MAPK/AKT signaling modulation of apoptotic activation and autophagic flux regulation. Frontiers in immunology, 2025 (PubMed: 40236708) [IF=5.7]

Application: WB    Species: human    Sample: Jurkat cell

Figure 4 Effects of M. oleifera leaves protein on Jurkat cell apoptosis and cyclin and autophagy protein expression. (a) Effects of M. oleifera leaves proteins on the expression of apoptosis, cyclin and autophagy proteins in Jurkat cells. (b) Bax/Bcl-2, (c) cleaved CasPase-3, (d) CyclinE1, (e) CyclinD1 and (f) ATG5 statistical analysis of protein expression. Compared with the blank control group (0 μg/mL), the differences between the groups are represented by *P
8). Chrysin induces autophagy-dependent ferroptosis to increase chemosensitivity to gemcitabine by targeting CBR1 in pancreatic cancer cells. Biochemical Pharmacology, 2021 (PubMed: 34673014) [IF=5.3]
9). Dexamethasone Promotes Autophagy Dependent Ferroptosis of Placental Trophoblast Cells Through GRα. Journal of cellular and molecular medicine, 2025 (PubMed: 40624893) [IF=5.3]

Application: WB    Species: human    Sample: placental tissues

FIGURE 5 DEX activated the AMPKα/BECN1 and ATG5/ATG7/NCOA4 signalling pathways via GRα. (A) Representative western blot results of placental tissues. (B) Representative western blot results showing HTR8/SVeno and TEV-1 expression. Data shown in the bar chart are presented as mean ± SD. Statistical analysis between two groups in (A) was performed by the student t-test, inter-group statistical analysis in (B) was performed by one-way ANOVA. ** in (A) represent p  0.05, and ** represent p 
10). The mechanism by which MALAT1/CREG1 regulates premature rupture of fetal membrane through autophagy mediated differentiation of amniotic fibroblasts. Non-coding RNA research, 2025 (PubMed: 40297152) [IF=5.0]
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