Product: EPHA4 Antibody
Catalog: AF5496
Description: Rabbit polyclonal antibody to EPHA4
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 110 kd; 110kD(Calculated).
Uniprot: P54764
RRID: AB_2837975

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%), Xenopus(86%)
Clonality:
Polyclonal
Specificity:
EPHA4 Antibody detects endogenous levels of total EPHA4.
RRID:
AB_2837975
Cite Format: Affinity Biosciences Cat# AF5496, RRID:AB_2837975.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Cek 8; CEK8; EK8; eph receptor a4; EPH-like kinase 8; EPHA4; EPHA4_HUMAN; Ephrin type-A receptor 4; HEK 8; hEK8; Receptor protein-tyrosine kinase HEK8; Sek 1; SEK; TYRO 1 protein tyrosine kinase; TYRO1; Tyrosine-protein kinase receptor SEK; Tyrosine-protein kinase TYRO1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P54764 EPHA4_HUMAN:

Ubiquitous.

Description:
Receptor tyrosine kinase which binds membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling.
Sequence:
MAGIFYFALFSCLFGICDAVTGSRVYPANEVTLLDSRSVQGELGWIASPLEGGWEEVSIMDEKNTPIRTYQVCNVMEPSQNNWLRTDWITREGAQRVYIEIKFTLRDCNSLPGVMGTCKETFNLYYYESDNDKERFIRENQFVKIDTIAADESFTQVDIGDRIMKLNTEIRDVGPLSKKGFYLAFQDVGACIALVSVRVFYKKCPLTVRNLAQFPDTITGADTSSLVEVRGSCVNNSEEKDVPKMYCGADGEWLVPIGNCLCNAGHEERSGECQACKIGYYKALSTDATCAKCPPHSYSVWEGATSCTCDRGFFRADNDAASMPCTRPPSAPLNLISNVNETSVNLEWSSPQNTGGRQDISYNVVCKKCGAGDPSKCRPCGSGVHYTPQQNGLKTTKVSITDLLAHTNYTFEIWAVNGVSKYNPNPDQSVSVTVTTNQAAPSSIALVQAKEVTRYSVALAWLEPDRPNGVILEYEVKYYEKDQNERSYRIVRTAARNTDIKGLNPLTSYVFHVRARTAAGYGDFSEPLEVTTNTVPSRIIGDGANSTVLLVSVSGSVVLVVILIAAFVISRRRSKYSKAKQEADEEKHLNQGVRTYVDPFTYEDPNQAVREFAKEIDASCIKIEKVIGVGEFGEVCSGRLKVPGKREICVAIKTLKAGYTDKQRRDFLSEASIMGQFDHPNIIHLEGVVTKCKPVMIITEYMENGSLDAFLRKNDGRFTVIQLVGMLRGIGSGMKYLSDMSYVHRDLAARNILVNSNLVCKVSDFGMSRVLEDDPEAAYTTRGGKIPIRWTAPEAIAYRKFTSASDVWSYGIVMWEVMSYGERPYWDMSNQDVIKAIEEGYRLPPPMDCPIALHQLMLDCWQKERSDRPKFGQIVNMLDKLIRNPNSLKRTGTESSRPNTALLDPSSPEFSAVVSVGDWLQAIKMDRYKDNFTAAGYTTLEAVVHVNQEDLARIGITAITHQNKILSSVQAMRTQMQQMHGRMVPV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
88
Xenopus
86
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P54764 As Substrate

Site PTM Type Enzyme
T117 Phosphorylation
T168 Phosphorylation
S349 Phosphorylation
S350 Phosphorylation
T354 Phosphorylation
T595 Phosphorylation
Y596 Phosphorylation P54764 (EPHA4)
T601 Phosphorylation
Y602 Phosphorylation P54764 (EPHA4)
S637 Phosphorylation
K693 Acetylation
K735 Ubiquitination
S741 Phosphorylation
Y779 Phosphorylation
T781 Phosphorylation
Y798 Phosphorylation
S887 Phosphorylation
T957 Phosphorylation

PTMs - P54764 As Enzyme

Substrate Site Source
P19174 (PLCG1) Y783 Uniprot
P54764-1 (EPHA4) Y596 Uniprot
P54764 (EPHA4) Y602 Uniprot
Q00535 (CDK5) Y15 Uniprot

Research Backgrounds

Function:

Receptor tyrosine kinase which binds membrane-bound ephrin family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Highly promiscuous, it has the unique property among Eph receptors to bind and to be physiologically activated by both GPI-anchored ephrin-A and transmembrane ephrin-B ligands including EFNA1 and EFNB3. Upon activation by ephrin ligands, modulates cell morphology and integrin-dependent cell adhesion through regulation of the Rac, Rap and Rho GTPases activity. Plays an important role in the development of the nervous system controlling different steps of axonal guidance including the establishment of the corticospinal projections. May also control the segregation of motor and sensory axons during neuromuscular circuit development. In addition to its role in axonal guidance plays a role in synaptic plasticity. Activated by EFNA1 phosphorylates CDK5 at 'Tyr-15' which in turn phosphorylates NGEF regulating RHOA and dendritic spine morphogenesis. In the nervous system, plays also a role in repair after injury preventing axonal regeneration and in angiogenesis playing a role in central nervous system vascular formation. Additionally, its promiscuity makes it available to participate in a variety of cell-cell signaling regulating for instance the development of the thymic epithelium. During development of the cochlear organ of Corti, regulates pillar cell separation by forming a ternary complex with ADAM10 and CADH1 which facilitates the cleavage of CADH1 by ADAM10 and disruption of adherens junctions (By similarity).

Subcellular Location:

Cell membrane>Single-pass type I membrane protein. Cell projection>Axon. Cell projection>Dendrite. Cell junction>Synapse>Postsynaptic density membrane. Early endosome. Cell junction>Adherens junction.
Note: Clustered upon activation and targeted to early endosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses. Interacts (phosphorylated at position Tyr-602) with FYN. Interacts with CDK5, CDK5R1 and NGEF; upon activation by EFNA1 induces NGEF phosphorylation by the kinase CDK5. Interacts with CHN1; effector of EPHA4 in axon guidance linking EPHA4 activation to RAC1 regulation (By similarity). Interacts (via PDZ motif) with SIPA1L1 (via PDZ domain); controls neuronal morphology through regulation of the RAP1 (RAP1A or RAP1B) and RAP2 (RAP2A, RAP2B or RAP2C) GTPases. Forms a ternary complex composed of ADAM10, CADH1 and EPHA4; within the complex, CADH1 is cleaved by ADAM10 which disrupts adherens junctions (By similarity).

Family&Domains:

The protein kinase domain mediates interaction with NGEF.

Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.

Research Fields

· Organismal Systems > Development > Axon guidance.   (View pathway)

References

1). Nogo-A-Δ20/EphA4 interaction antagonizes apoptosis of neural stem cells by integrating p38 and JNK MAPK signaling. JOURNAL OF MOLECULAR HISTOLOGY, 2021 (PubMed: 33555537) [IF=3.2]

Application: IF/ICC    Species: rat    Sample: neural stem cells

Fig. 1 Co-expression of Nogo-A-Δ20 and EphA4 in neural stem cells. a The monoclonal antibody (mAb) A563 against aa 563–627 (amino acids (aa) 563–627) of rat Nogo-A-NiG-Δ20, which is the main inhibitory region of rat Nogo-A. Immunofuorescence staining demonstrate that NSCs spheres are both positive for A563 and EphA4. Co- expression regions are labeled in yellow in the merged images. Staining of Hoechst 33,258 to show the nuclei. Scale bar 50 μm. b We dissociated and plated the neurospheres as single cells and performed Nogo-A and EphA4 double immunostaining. Colocalization are presented in yellow in the merged images. These were colocalization restricted to NSCs growing in the presence of growth factors. Scale bar 100 μm. c–f Western blot analysis detecting NogoA (~135  kDa), EphA4 (~110  kDa), GFAP (~50  kDa) and β-actin (~43 kDa) proteins in supernatants collected from lysis solutions of neurospheres. All bands presented are representative images of at three independent experiments. M marker. g The diagram represents the three inhibitory regions of rat Nogo-A, which are Nogo-A NiR- Δ2 (amino acids [aa] 59–172), NiG-Δ20 (aa 544–725) and Nogo-66 (aa 1019–1083), respectively. The diferent antigen recognition sites can be recognized by various antibody

Application: WB    Species: rat    Sample: neural stem cells

Fig. 1 Co-expression of Nogo-A-Δ20 and EphA4 in neural stem cells. a The monoclonal antibody (mAb) A563 against aa 563–627 (amino acids (aa) 563–627) of rat Nogo-A-NiG-Δ20, which is the main inhibitory region of rat Nogo-A. Immunofuorescence staining demonstrate that NSCs spheres are both positive for A563 and EphA4. Co- expression regions are labeled in yellow in the merged images. Staining of Hoechst 33,258 to show the nuclei. Scale bar 50 μm. b We dissociated and plated the neurospheres as single cells and performed Nogo-A and EphA4 double immunostaining. Colocalization are presented in yellow in the merged images. These were colocalization restricted to NSCs growing in the presence of growth factors. Scale bar 100 μm. c–f Western blot analysis detecting NogoA (~135  kDa), EphA4 (~110  kDa), GFAP (~50  kDa) and β-actin (~43 kDa) proteins in supernatants collected from lysis solutions of neurospheres. All bands presented are representative images of at three independent experiments. M marker. g The diagram represents the three inhibitory regions of rat Nogo-A, which are Nogo-A NiR- Δ2 (amino acids [aa] 59–172), NiG-Δ20 (aa 544–725) and Nogo-66 (aa 1019–1083), respectively. The diferent antigen recognition sites can be recognized by various antibody

2). Short-term high-fat diet favors the appearances of apoptosis and gliosis by activation of ERK1/2/p38MAPK pathways in brain. Imaging, 2021 (PubMed: 34620734) [IF=0.4]

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.