DNMT3B Antibody - #AF5493

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Product: DNMT3B Antibody
Catalog: AF5493
Description: Rabbit polyclonal antibody to DNMT3B
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Rabbit
Mol.Wt.: 97 kDa; 96kD(Calculated).
Uniprot: Q9UBC3
RRID: AB_2837973

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(82%), Horse(82%), Sheep(82%), Rabbit(91%)
Clonality:
Polyclonal
Specificity:
DNMT3B Antibody detects endogenous levels of total DNMT3B.
RRID:
AB_2837973
Cite Format: Affinity Biosciences Cat# AF5493, RRID:AB_2837973.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Cytosine 5methyltransferase 3B; DNA; DNA (cytosine 5) methyltransferase 3 beta; DNA (cytosine 5)-methyltransferase 3B; DNA (cytosine-5)-methyltransferase 3B; DNA methyltransferase HsaIIIB; DNA MTase HsaIIIB; DNM3B_HUMAN; Dnmt3b; EC 2.1.1.37; ICF; ICF1; M.HsaIIIB; MGC124407; RP23-89H14.3;

Immunogens

Immunogen:
Uniprot:

Q9UBC3(DNM3B_HUMAN) >>Visit HPA database.

Gene(ID):
DNMT3B(1789)
Expression:
Q9UBC3 DNM3B_HUMAN:

Ubiquitous; highly expressed in fetal liver, heart, kidney, placenta, and at lower levels in spleen, colon, brain, liver, small intestine, lung, peripheral blood mononuclear cells, and skeletal muscle. Isoform 1 is expressed in all tissues except brain, skeletal muscle and PBMC, 3 is ubiquitous, 4 is expressed in all tissues except brain, skeletal muscle, lung and prostate and 5 is detectable only in testis and at very low level in brain and prostate.

Description:
Required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region.
Sequence:
MKGDTRHLNGEEDAGGREDSILVNGACSDQSSDSPPILEAIRTPEIRGRRSSSRLSKREVSSLLSYTQDLTGDGDGEDGDGSDTPVMPKLFRETRTRSESPAVRTRNNNSVSSRERHRPSPRSTRGRQGRNHVDESPVEFPATRSLRRRATASAGTPWPSPPSSYLTIDLTDDTEDTHGTPQSSSTPYARLAQDSQQGGMESPQVEADSGDGDSSEYQDGKEFGIGDLVWGKIKGFSWWPAMVVSWKATSKRQAMSGMRWVQWFGDGKFSEVSADKLVALGLFSQHFNLATFNKLVSYRKAMYHALEKARVRAGKTFPSSPGDSLEDQLKPMLEWAHGGFKPTGIEGLKPNNTQPVVNKSKVRRAGSRKLESRKYENKTRRRTADDSATSDYCPAPKRLKTNCYNNGKDRGDEDQSREQMASDVANNKSSLEDGCLSCGRKNPVSFHPLFEGGLCQTCRDRFLELFYMYDDDGYQSYCTVCCEGRELLLCSNTSCCRCFCVECLEVLVGTGTAAEAKLQEPWSCYMCLPQRCHGVLRRRKDWNVRLQAFFTSDTGLEYEAPKLYPAIPAARRRPIRVLSLFDGIATGYLVLKELGIKVGKYVASEVCEESIAVGTVKHEGNIKYVNDVRNITKKNIEEWGPFDLVIGGSPCNDLSNVNPARKGLYEGTGRLFFEFYHLLNYSRPKEGDDRPFFWMFENVVAMKVGDKRDISRFLECNPVMIDAIKVSAAHRARYFWGNLPGMNRPVIASKNDKLELQDCLEYNRIAKLKKVQTITTKSNSIKQGKNQLFPVVMNGKEDVLWCTELERIFGFPVHYTDVSNMGRGARQKLLGRSWSVPVIRHLFAPLKDYFACE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
91
Horse
82
Bovine
82
Sheep
82
Pig
67
Dog
67
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UBC3 As Substrate

Site PTM Type Enzyme
S51 Phosphorylation
S52 Phosphorylation
S53 Phosphorylation
S56 Phosphorylation
S82 Phosphorylation
T96 Phosphorylation
S98 Phosphorylation
S100 Phosphorylation
T105 Phosphorylation
S110 Phosphorylation
S112 Phosphorylation
S113 Phosphorylation
S120 Phosphorylation
S136 Phosphorylation
S195 Phosphorylation
S202 Phosphorylation
S209 Phosphorylation
S270 Phosphorylation
Y303 Phosphorylation
S320 Phosphorylation
T353 Phosphorylation
T383 Phosphorylation
S387 Phosphorylation
K397 Ubiquitination
T401 Phosphorylation
Y404 Phosphorylation
S416 Phosphorylation
K428 Ubiquitination
K562 Ubiquitination
Y588 Phosphorylation
K597 Ubiquitination
K623 Ubiquitination
K662 Ubiquitination
S711 Phosphorylation
K777 Ubiquitination
S780 Phosphorylation
Y815 Phosphorylation
T816 Phosphorylation
S819 Phosphorylation
R823 Methylation
R826 Methylation
S835 Phosphorylation
K847 Ubiquitination

Research Backgrounds

Function:

Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing (By similarity). In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs. Functions as a transcriptional corepressor by associating with ZHX1. Required for DUX4 silencing in somatic cells.

PTMs:

Sumoylated.

Citrullinated by PADI4.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous; highly expressed in fetal liver, heart, kidney, placenta, and at lower levels in spleen, colon, brain, liver, small intestine, lung, peripheral blood mononuclear cells, and skeletal muscle. Isoform 1 is expressed in all tissues except brain, skeletal muscle and PBMC, 3 is ubiquitous, 4 is expressed in all tissues except brain, skeletal muscle, lung and prostate and 5 is detectable only in testis and at very low level in brain and prostate.

Subunit Structure:

Interacts with BAZ2A/TIP5, SUV39H1 and CBX4. Interacts with UHRF1 (By similarity). Interacts with DNMT1 and DNMT3A, SETDB1, UBL1, UBE2I9 and ZHX1. Interacts with the PRC2/EED-EZH2 complex.

Family&Domains:

The PWWP domain is essential for targeting to pericentric heterochromatin.

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Research Fields

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). Inhibitory effect of bound polyphenol from foxtail millet bran on miR-149 methylation increases the chemosensitivity of human colorectal cancer HCT-8/Fu cells. MOLECULAR AND CELLULAR BIOCHEMISTRY, 2021 (PubMed: 33011952) [IF=4.3]

Application: WB    Species: human    Sample: HCT-8 and HCT-8/Fu cells

Fig. 4 |Efects of BPIS on the expression of DNA methylation-related enzymes. a, b The expressions of DNMT3a, DNMT3b and MECP2 were measured in RNA and protein levels in HCT-8 and HCT-8/Fu cells by RT-PCR and western blotting assays.
2). Decreased ovarian function and autophagy gene methylation in aging rats. Journal of Ovarian Research, 2020 (PubMed: 32014030) [IF=4.0]

Application: WB    Species: rat    Sample: ovaries

Fig. 6| Protein expression of Dnmt1, Dnmt2, Dnmt3A and Dnmt3B in the ovaries of rats.(a) Western blot results.
3). The mechanisms underlying olanzapine-induced insulin resistance via the brown adipose tissue and the therapy in rats. Adipocyte, 2022 (PubMed: 35067163) [IF=3.3]
4). Regulation of RUNX3 Expression by DNA Methylation in Prostate Cancer [Retraction]. Cancer Management and Research, 2021 (PubMed: 35002319) [IF=3.3]

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