TSG6 Antibody - #AF5492

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Product: TSG6 Antibody
Catalog: AF5492
Description: Rabbit polyclonal antibody to TSG6
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 31 kDa; 31kD(Calculated).
Uniprot: P98066
RRID: AB_2837972

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(89%)
Clonality:
Polyclonal
Specificity:
TSG6 Antibody detects endogenous levels of total TSG6.
RRID:
AB_2837972
Cite Format: Affinity Biosciences Cat# AF5492, RRID:AB_2837972.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Hyaluronate binding protein; Hyaluronate-binding protein; TNF alpha induced protein 6; TNF alpha-induced protein 6; TNF stimulated gene 6 protein; TNF-stimulated gene 6 protein; TNFAIP 6; Tnfaip6; TSG 6; TSG-6; TSG6_HUMAN; Tumor necrosis factor alpha induced protein 6; Tumor necrosis factor alpha inducible protein 6; Tumor necrosis factor alpha-induced protein 6; Tumor necrosis factor inducible gene 6 protein; Tumor necrosis factor stimulated gene 6 protein; Tumor necrosis factor-inducible gene 6 protein;

Immunogens

Immunogen:
Uniprot:

P98066(TSG6_HUMAN) >>Visit HPA database.

Gene(ID):
TNFAIP6(7130)
Expression:
P98066 TSG6_HUMAN:

Found in the synovial fluid of patients with rheumatoid arthritis.

Description:
Possibly involved in cell-cell and cell-matrix interactions during inflammation and tumorigenesis.Subunit structure Interacts with inter-alpha-inhibitor (I-alpha-I). Chondroitin sulfate may be required for the stability of the complex.
Sequence:
MIILIYLFLLLWEDTQGWGFKDGIFHNSIWLERAAGVYHREARSGKYKLTYAEAKAVCEFEGGHLATYKQLEAARKIGFHVCAAGWMAKGRVGYPIVKPGPNCGFGKTGIIDYGIRLNRSERWDAYCYNPHAKECGGVFTDPKQIFKSPGFPNEYEDNQICYWHIRLKYGQRIHLSFLDFDLEDDPGCLADYVEIYDSYDDVHGFVGRYCGDELPDDIISTGNVMTLKFLSDASVTAGGFQIKYVAMDPVSKSSQGKNTSTTSTGNKNFLAGRFSHL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Rabbit
100
Dog
89
Xenopus
78
Zebrafish
67
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P98066 As Substrate

Site PTM Type Enzyme
Y38 Phosphorylation
S148 Phosphorylation
Y155 Phosphorylation
T259 Phosphorylation
T262 Phosphorylation

Research Backgrounds

Function:

Possibly involved in cell-cell and cell-matrix interactions during inflammation and tumorigenesis.

Tissue Specificity:

Found in the synovial fluid of patients with rheumatoid arthritis.

Subunit Structure:

Interacts with inter-alpha-inhibitor (I-alpha-I). Chondroitin sulfate may be required for the stability of the complex.

References

1). A novel therapeutic approach for inflammatory bowel disease by exosomes derived from human umbilical cord mesenchymal stem cells to repair intestinal barrier via TSG-6. Stem Cell Research & Therapy, 2021 (PubMed: 34051868) [IF=7.5]

Application: WB    Species: Mice    Sample: colon tissue

Fig. 7 MSCs-Exo enhanced TSG-6 expression in the colitis mice colon. a TSG-6 expression levels in colon tissue in the control, DSS + PBS, and DSS + Exo groups 10 days after colitis induction. b TSG-6 expression in the DSS + siNC Exo and DSS + siTSG-6 Exo groups. c TSG-6 protein expression in MSC-Exo. d The levels of TSG-6 in MSC-Exo and supernatant without exosomes. Exosomes in the supernatant were removed by ultrafiltration. All samples were adjusted to the same protein concentration, followed by ELISA. Data are presented as mean ± SD; n = 4 per group. *P < 0.05, **P < 0.01 and ns indicates P > 0.05
2). Identification and validation of hub genes in drug induced acute kidney injury basing on integrated transcriptomic analysis. Frontiers in Immunology, 2023 (PubMed: 37063876) [IF=7.3]
3). Tumor necrosis factor-stimulated gene-6 ameliorates early brain injury after subarachnoid hemorrhage by suppressing NLRC4 inflammasome-mediated astrocyte pyroptosis. Neural Regeneration Research, 2024 (PubMed: 37862209) [IF=6.1]

Application: WB    Species: Mouse    Sample:

Figure 1 Representative photographs of SAH mice and the expression levels of TSG-6, NLRC4, GSDMD, and GSDMD-N after SAH. (A) Representative photographs of a sham and subarachnoid hemorrhage (SAH) mouse brain 24 hours after model construction. (B) Representative photographs of hematoxylin and eosin-stained slides of sham cerebral cortex and cortex 24 hours after SAH. Scale bar: 50 μm. (C, E) Western blot bands and quantification of TSG-6, NLRC4, GSDMD, and GSDMD-N proteins in sham mice and at 6, 12, 24, 48, and 72 hours after SAH. (D) Representative immunohistochemistry staining results for TSG-6 in the cortex of sham and SAH mice 24 hours after model construction. Scale bar: 50 μm. Values shown are means ± standard deviation of 6 samples from each group. **P < 0.01, ***P < 0.001, vs. sham group (one-way analysis of variance with Tukey’s honestly significant difference test). GSDMD: Gasdermin-D; NLRC4: NLR family CARD domain containing 4; SAH: subarachnoid hemorrhage; TSG-6: Tumor necrosis factor-stimulated gene-6; WB: western blot.

Application: IF/ICC    Species: Mouse    Sample:

Figure 2 TSG-6 is expressed in astrocytes and neurons after subarachnoid hemorrhage (SAH). (A) Representative co-immunofluorescence (IF) images showing TSG-6 (green) in astrocytes (GFAP, red) of sham mice. (B) Representative co-IF images showing TSG-6 (green) in astrocytes (GFAP, red), microglia (Iba-1, red), and neurons (NeuN, red) of SAH mice 24 hours after SAH. Scale bars: 50 μm. (C, D) Quantification of IF staining results. Values shown are means ± standard deviation of 6 samples from each group. ****P < 0.0001 (one-way analysis of variance with Tukey’s honestly significant difference test [D] or Student’s t-test [C]). DAPI: 4′,6-Diamidino-2-phenylindole; GFAP: glial fibrillary acidic protein; Iba-1: ionized calcium-binding adapter molecule 1; IF: immunofluorescence; NeuN: neuronal nuclei antigen; SAH: subarachnoid hemorrhage; TSG-6: Tumor necrosis factor-stimulated gene-6.
4). Identification of Neural Progenitor Cell-associated Chemoradiotherapy Resistance Gene Set (ARL4C, MSN, TNFAIP6) for Prognosis of Glioma. Current Pharmaceutical Design, 2019 [IF=3.1]

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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