PGP9.5 Antibody - #AF5490

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Product: PGP9.5 Antibody
Catalog: AF5490
Description: Rabbit polyclonal antibody to PGP9.5
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 25 kDa; 25kD(Calculated).
Uniprot: P09936
RRID: AB_2837970

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(83%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(83%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
PGP9.5 Antibody detects endogenous levels of total PGP9.5.
RRID:
AB_2837970
Cite Format: Affinity Biosciences Cat# AF5490, RRID:AB_2837970.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Epididymis luminal protein 117; Epididymis secretory protein Li 53; HEL 117; HEL S 53; NDGOA; Neuron cytoplasmic protein 9.5; OTTHUMP00000218137; OTTHUMP00000218139; OTTHUMP00000218140; OTTHUMP00000218141; Park 5; PARK5; PGP 9.5; PGP9.5; PGP95; Protein gene product 9.5; Ubiquitin C terminal esterase L1; Ubiquitin C terminal hydrolase; Ubiquitin C terminal hydrolase L1; Ubiquitin carboxyl terminal esterase L1; Ubiquitin carboxyl terminal hydrolase isozyme L1; Ubiquitin carboxyl-terminal hydrolase isozyme L1; Ubiquitin thioesterase L1; Ubiquitin thiolesterase; Ubiquitin thiolesterase L1; UCH-L1; UCHL1; UCHL1_HUMAN;

Immunogens

Immunogen:
Uniprot:

P09936(UCHL1_HUMAN) >>Visit HPA database.

Gene(ID):
UCHL1(7345)
Expression:
P09936 UCHL1_HUMAN:

Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.

Description:
Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes.
Sequence:
MQLKPMEINPEMLNKVLSRLGVAGQWRFVDVLGLEEESLGSVPAPACALLLLFPLTAQHENFRKKQIEELKGQEVSPKVYFMKQTIGNSCGTIGLIHAVANNQDKLGFEDGSVLKQFLSETEKMSPEDRAKCFEKNEAIQAAHDAVAQEGQCRVDDKVNFHFILFNNVDGHLYELDGRMPFPVNHGASSEDTLLKDAAKVCREFTEREQGEVRFSAVALCKAA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
92
Zebrafish
83
Chicken
83
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P09936 As Substrate

Site PTM Type Enzyme
K4 Acetylation
K4 Ubiquitination
K15 Ubiquitination
K64 Ubiquitination
K65 Ubiquitination
K71 Ubiquitination
K78 Ubiquitination
K83 Ubiquitination
K105 Ubiquitination
K115 Ubiquitination
S119 Phosphorylation
K123 Ubiquitination
C132 S-Nitrosylation
K135 Ubiquitination
C152 S-Nitrosylation
K157 Ubiquitination
S188 Phosphorylation
S189 Phosphorylation
T192 Phosphorylation
K195 Acetylation
K195 Ubiquitination
C220 S-Nitrosylation
K221 Ubiquitination

Research Backgrounds

Function:

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins (Probable). This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes (By similarity). The homodimer may have ATP-independent ubiquitin ligase activity.

PTMs:

O-glycosylated.

Subcellular Location:

Cytoplasm. Endoplasmic reticulum membrane>Lipid-anchor.
Note: About 30% of total UCHL1 is associated with membranes in brain.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.

Subunit Structure:

Monomer. Homodimer. Interacts with SNCA (By similarity). Interacts with COPS5.

Family&Domains:

Belongs to the peptidase C12 family.

Research Fields

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

References

1). UCHL1 promotes the proliferation of porcine granulosa cells by stabilizing CCNB1. Journal of animal science and biotechnology, 2024 (PubMed: 38858680) [IF=7.0]
2). Deubiquitinase UCHL1 regulates estradiol synthesis by stabilizing voltage-dependent anion channel 2. Journal of Biological Chemistry, 2023 (PubMed: 37797697) [IF=4.8]
3). The Effect of Shaoyao Gancao Decoction on Sphincter of Oddi Dysfunction in Hypercholesterolemic Rabbits via Protecting the Enteric Nervous System–Interstitial Cells of Cajal–Smooth Muscle Cells Network. Journal of Inflammation Research, 2021 (PubMed: 34552344) [IF=4.5]

Application: IF/ICC    Species: Rabbit    Sample: SO tissue

Figure 4 (A) Normal group, (B) Model group, (C) Treatment group: Rabbit SO tissue, nerve fiber PGP9.5 in the inner muscular layer detected by laser confocal microscopy, red light is PGP9.5, blue light is DAPI. (D) Normal group, (E) Model group, (F) SGD treatment group: α-actin labeled rabbit SO tissues were examined by laser confocal microscopy (400×, 20 um), with α-actin in red light and DAPI in blue light. (G) fluorescence intensity of PGP9.5 (IOD) in the SO tissue of rabbits in each group; (H) The fluorescence intensity (IOD) of α-actin labeled SMC in the SO tissue of rabbits in each group. Arrows indicate positive expression.*** indicates the comparison between the model group and the control group, P<0.05, ##indicates the comparison between the model group and the treatment group, P<0.05. In α-action, ** indicates the comparison between the model group and the control group, P<0.05, # indicates the comparison between the model group and the treatment group, P<0.05.

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