Cytokeratin 17 Antibody - #AF5480

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Product: Cytokeratin 17 Antibody
Catalog: AF5480
Description: Rabbit polyclonal antibody to Cytokeratin 17
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 48 kDa; 48kD(Calculated).
Uniprot: Q04695
RRID: AB_2837962

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(82%), Bovine(94%), Horse(94%), Sheep(94%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Cytokeratin 17 Antibody detects endogenous levels of total Cytokeratin 17.
RRID:
AB_2837962
Cite Format: Affinity Biosciences Cat# AF5480, RRID:AB_2837962.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

39.1; CK 17; CK-17; Cytokeratin-17; K17; K1C17_HUMAN; Keratin 17; keratin 17 epitope S1; keratin 17 epitope S2; keratin 17 epitope S4; Keratin 17, type I; Keratin; Keratin type I cytoskeletal 17; keratin, type i cytoskeletal 17 [version 1]; Keratin-17; KRT17; PC; PC2; PCHC1; type I cytoskeletal 17;

Immunogens

Immunogen:
Uniprot:

Q04695(K1C17_HUMAN) >>Visit HPA database.

Gene(ID):
KRT17(3872)
Expression:
Q04695 K1C17_HUMAN:

Expressed in the outer root sheath and medulla region of hair follicle specifically from eyebrow and beard, digital pulp, nail matrix and nail bed epithelium, mucosal stratified squamous epithelia and in basal cells of oral epithelium, palmoplantar epidermis and sweat and mammary glands. Also expressed in myoepithelium of prostate, basal layer of urinary bladder, cambial cells of sebaceous gland and in exocervix (at protein level).

Description:
May play a role in the formation and maintenance of various skin appendages, specifically in determining shape and orientation of hair. May be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial stem cells.
Sequence:
MTTSIRQFTSSSSIKGSSGLGGGSSRTSCRLSGGLGAGSCRLGSAGGLGSTLGGSSYSSCYSFGSGGGYGSSFGGVDGLLAGGEKATMQNLNDRLASYLDKVRALEEANTELEVKIRDWYQRQAPGPARDYSQYYRTIEELQNKILTATVDNANILLQIDNARLAADDFRTKFETEQALRLSVEADINGLRRVLDELTLARADLEMQIENLKEELAYLKKNHEEEMNALRGQVGGEINVEMDAAPGVDLSRILNEMRDQYEKMAEKNRKDAEDWFFSKTEELNREVATNSELVQSGKSEISELRRTMQALEIELQSQLSMKASLEGNLAETENRYCVQLSQIQGLIGSVEEQLAQLRCEMEQQNQEYKILLDVKTRLEQEIATYRRLLEGEDAHLTQYKKEPVTTRQVRTIVEEVQDGKVISSREQVHQTTR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Dog
100
Horse
94
Bovine
94
Sheep
94
Zebrafish
82
Xenopus
67
Pig
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q04695 As Substrate

Site PTM Type Enzyme
M1 Acetylation
T2 Phosphorylation
S10 Phosphorylation
S11 Phosphorylation
S12 Phosphorylation
S13 Phosphorylation
K15 Methylation
K15 Sumoylation
K15 Ubiquitination
S17 Phosphorylation
S18 Phosphorylation
S24 Phosphorylation
S25 Phosphorylation
T27 Phosphorylation
S28 Phosphorylation
S32 Phosphorylation
S39 Phosphorylation
S44 Phosphorylation
Y69 Phosphorylation
S97 Phosphorylation
Y98 Phosphorylation
K101 Acetylation
K101 Ubiquitination
T110 Phosphorylation
K115 Sumoylation
K115 Ubiquitination
Y120 Phosphorylation
S132 Phosphorylation
Y134 Phosphorylation
Y135 Phosphorylation
T171 Phosphorylation
K172 Methylation
K172 Sumoylation
K172 Ubiquitination
T175 Phosphorylation
Y217 Phosphorylation
K219 Sumoylation
K219 Ubiquitination
K220 Ubiquitination
Y260 Phosphorylation
K262 Ubiquitination
K269 Ubiquitination
S277 Phosphorylation
K278 Methylation
K278 Sumoylation
K278 Ubiquitination
T288 Phosphorylation
S290 Phosphorylation
S295 Phosphorylation
K297 Ubiquitination
S301 Phosphorylation
S323 Phosphorylation
Y367 Phosphorylation
K368 Ubiquitination
K374 Ubiquitination
T383 Phosphorylation
Y384 Phosphorylation
Y398 Phosphorylation
K399 Sumoylation
K399 Ubiquitination
K400 Sumoylation
K400 Ubiquitination
T410 Phosphorylation
K419 Sumoylation
K419 Ubiquitination

Research Backgrounds

Function:

Type I keratin involved in the formation and maintenance of various skin appendages, specifically in determining shape and orientation of hair (By similarity). Required for the correct growth of hair follicles, in particular for the persistence of the anagen (growth) state (By similarity). Modulates the function of TNF-alpha in the specific context of hair cycling. Regulates protein synthesis and epithelial cell growth through binding to the adapter protein SFN and by stimulating Akt/mTOR pathway (By similarity). Involved in tissue repair. May be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial 'stem cells'. Acts as a promoter of epithelial proliferation by acting a regulator of immune response in skin: promotes Th1/Th17-dominated immune environment contributing to the development of basaloid skin tumors (By similarity). May act as an autoantigen in the immunopathogenesis of psoriasis, with certain peptide regions being a major target for autoreactive T-cells and hence causing their proliferation.

PTMs:

Phosphorylation at Ser-44 occurs in a growth- and stress-dependent fashion in skin keratinocytes, it has no effect on filament organization.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in the outer root sheath and medulla region of hair follicle specifically from eyebrow and beard, digital pulp, nail matrix and nail bed epithelium, mucosal stratified squamous epithelia and in basal cells of oral epithelium, palmoplantar epidermis and sweat and mammary glands. Also expressed in myoepithelium of prostate, basal layer of urinary bladder, cambial cells of sebaceous gland and in exocervix (at protein level).

Subunit Structure:

Heterodimer of a type I and a type II keratin. KRT17 associates with KRT6 isomers (KRT6A or KRT6B). Interacts with TRADD and SFN (By similarity).

Family&Domains:

Belongs to the intermediate filament family.

Research Fields

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

References

1). KRT17 Accelerates Cell Proliferative and Invasive Potential of Laryngeal Squamous Cell Carcinoma (LSCC) through Regulating AKT/mTOR and Wnt/β-Catenin Pathways. Evidence-based Complementary and Alternative Medicine, 2022 (PubMed: 36248412)

Application: WB    Species: Human    Sample: LSCC tissues

Figure 1 KRT17 expression is elevated in LSCC tissues and cells and is linked to poor prognosis. (a, b) QRT-PCR detected KRT17 mRNA expression in LSCC and nontumor tissues (n = 42). (c) Western blot detected KRT17 protein level in LSCC and nontumor tissues (n = 5). (d) Overall survival of LSCC patients with high or low level of KRT17. (e) qRT-PCR assessed KRT17 mRNA in AMC-HN-8, TU686, TU177, TU212, and 16HBE cells. (f) Western blot detected KRT17 protein level in AMC-HN-8, TU686, TU177, TU212, and 16HBE cells. ∗A significant difference compared with the normal group or 16HBE group. ∗∗P < 0.01. Abbreviations: N, nontumor tissues; T, LSCC tissues.

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