GLUT3 Antibody - #AF5463

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Product: GLUT3 Antibody
Catalog: AF5463
Description: Rabbit polyclonal antibody to GLUT3
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Horse, Chicken
Mol.Wt.: 54 kDa; 54kD(Calculated).
Uniprot: P11169
RRID: AB_2837947

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Horse(83%), Chicken(89%)
Clonality:
Polyclonal
Specificity:
GLUT3 Antibody detects endogenous levels of total GLUT3.
RRID:
AB_2837947
Cite Format: Affinity Biosciences Cat# AF5463, RRID:AB_2837947.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

brain; FLJ90380; Glucose transporter type 3; Glucose transporter type 3 brain; GLUT 3; GLUT-3; GLUT3; GTR3_HUMAN; Slc2a3; Solute Carrier Family 2 (Facilitated Glucose Transporter) Member 3; Solute carrier family 2, facilitated glucose transporter member 3;

Immunogens

Immunogen:
Uniprot:

P11169(GTR3_HUMAN) >>Visit HPA database.

Gene(ID):
SLC2A3(6515)
Expression:
P11169 GTR3_HUMAN:

Highly expressed in brain. Expressed in many tissues.

Description:
Tissue specificityHighly expressed in brain. Expressed in many tissues.Sequence similaritiesBelongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
Sequence:
MGTQKVTPALIFAITVATIGSFQFGYNTGVINAPEKIIKEFINKTLTDKGNAPPSEVLLTSLWSLSVAIFSVGGMIGSFSVGLFVNRFGRRNSMLIVNLLAVTGGCFMGLCKVAKSVEMLILGRLVIGLFCGLCTGFVPMYIGEISPTALRGAFGTLNQLGIVVGILVAQIFGLEFILGSEELWPLLLGFTILPAILQSAALPFCPESPRFLLINRKEEENAKQILQRLWGTQDVSQDIQEMKDESARMSQEKQVTVLELFRVSSYRQPIIISIVLQLSQQLSGINAVFYYSTGIFKDAGVQEPIYATIGAGVVNTIFTVVSLFLVERAGRRTLHMIGLGGMAFCSTLMTVSLLLKDNYNGMSFVCIGAILVFVAFFEIGPGPIPWFIVAELFSQGPRPAAMAVAGCSNWTSNFLVGLLFPSAAHYLGAYVFIIFTGFLITFLAFTFFKVPETRGRTFEDITRAFEGQAHGADRSGKDGVMEMNSIEPAKETTTNV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Chicken
89
Horse
83
Bovine
70
Sheep
70
Dog
67
Rabbit
64
Pig
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P11169 As Substrate

Site PTM Type Enzyme
K223 Ubiquitination
T232 Phosphorylation
K243 Ubiquitination
S250 Phosphorylation
K253 Ubiquitination
T333 Phosphorylation
S352 Phosphorylation
K477 Ubiquitination
S485 Phosphorylation
K490 Ubiquitination

Research Backgrounds

Function:

Facilitative glucose transporter that can also mediate the uptake of various other monosaccharides across the cell membrane. Mediates the uptake of glucose, 2-deoxyglucose, galactose, mannose, xylose and fucose, and probably also dehydroascorbate. Does not mediate fructose transport.

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Perikaryon. Cell projection.
Note: Localized to densely spaced patches along neuronal processes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in brain. Expressed in many tissues.

Family&Domains:

Transport is mediated via a series of conformation changes, switching between a conformation where the substrate-binding cavity is accessible from the outside, and a another conformation where it is accessible from the cytoplasm.

Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.

References

1). Recurrent moderate hypoglycemia accelerates the progression of cognitive deficits through impairment of TRPC6/GLUT3 pathway in diabetic APP/PS1 mice. JCI Insight, 2022 (PubMed: 35077394) [IF=8.0]

Application: WB    Species: Mice    Sample: hippocampal homogenates

Figure 5 RH reduced brain GLUT3-mediated glucose uptake in STZ-induced APP/PS1-DM mice. (A) Representative PET/CT images showing in vivo brain 18F-FDG uptake (coronal, sagittal, and transaxial sections, n = 6 mice for each group). Cor, Cortex; Hip, hippocampus; Mid, midbrain; HPOA, hypothalamus; BS, brain stem; Cer, cerebellum; OB, Olfactory Bulb; BF, basal forebrain; Tha, thalamus; SC, superior colliculi; CG, central gray; Str, striatum. (B) Quantification for SUV of 18F-FDG in brain region (n = 6 for mice for each group). (C) Western blot and quantitation for GLUT1, GLUT2, GLUT3, GLUT4, GLUT5, SGLT1, and SGLT2 in hippocampal homogenates (n = 3 mice for each group). The data are expressed as the mean ± SEM. Statistical significance was assessed using unpaired Student’s t test. *P < 0.05 and **P < 0.01, APP/PS1-DM versus APP/PS1; #P < 0.05 and ##P < 0.01, APP/PS1-DM-RH versus APP/PS1-DM; WT versus APP/PS1; NS, no significant difference.
2). Icariin ameliorates memory deficits through regulating brain insulin signaling and glucose transporters in 3× Tg-AD mice. Neural Regeneration Research, 2023 (PubMed: 35799540) [IF=6.1]

Application: WB    Species: Mice    Sample: cerebral cortex

Figure 6 Effects of ICA on glucose transporters in the cerebral cortex of 3×Tg-AD mice. The protein expression of GLUT1 (A) and GLUT3 (B) in the cerebral cortex. The expression levels were normalized to those in the WT + vehicle group. The data are presented as the means ± SEM (n = 4–5). *P < 0.05, vs. WT + vehicle group; #P < 0.05, vs. 3×Tg-AD + vehicle group (one-way analysis of variance followed by the least significant difference test). 3×Tg-AD: A triple-transgenic mouse model of Alzheimer’s disease; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; GLUT: glucose transporter; ICA: icariin; WT: wild-type.
3). Regular Exercise Enhances Cognitive Function and Intracephalic GLUT Expression in Alzheimer\'s Disease Model Mice. JOURNAL OF ALZHEIMERS DISEASE, 2019 (PubMed: 31561359) [IF=4.0]

Application: WB    Species: mouse    Sample: hippocampus

Fig. 6. GLUT1 and GLUT3 expression. Representative immunoblots of GLUT1 (A, B) and GLUT3 (C, D) in the cortex and hippocampus in each group. Protein immunoreactivity was normalized to -actin. Individual data are presented as the mean ± S.E.M. from 5 individual mice in each group.
4). Yes associated protein 1 promotes resistance to 5-fluorouracil in gastric cancer by regulating GLUT3-dependent glycometabolism reprogramming of tumor-associated macrophages. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2021 (PubMed: 33727040) [IF=3.9]

Application: WB    Species: Mice    Sample: GC cells

Fig. 3. IL13 secreted by YAP1-overexpressed GC cells stimulates resistance to 5-FU via inducing M2 subtype macrophage glycolysis reprogramming. A-C. RT-PCR was used to detect the mRNA expression of glycolysis enzymes, fatty acid and amino acid metabolism enzymes in THP1 after co-cultured with MKN-YAP1 or MKN45-Vetor. D. Protein level change of glycolysis enzymes in THP1 after co-cultured with MKN-YAP1, MKN45-Vetor, SGC7901-siYAP1 or SGC7901-NC. E. RT-PCR revealed the mRNA expression of glycolysis enzymes and M2 TAMs markers in THP1 after co-cultured with SGC7901-siYAP1 or SGC7901-NC. F-G. Relative lactate release from cells was determined by colorimetric analysis. Relative glucose uptake cells by Flow cytometry detection. All data presented are the mean ± SD (*p < 0.05, **p < 0.01) of triplicate determination from three independent experiments.
5). Zinc Regulates Glucose Metabolism of the Spinal Cord and Neurons and Promotes Functional Recovery after Spinal Cord Injury through the AMPK Signaling Pathway. Oxidative Medicine and Cellular Longevity, 2021 (PubMed: 34373765)

Application: IF/ICC    Species: Mice    Sample: PC12 Neurons

Figure 2 Zinc promoted glucose transport in PC12 neurons. (a–c) The expression levels of GLUT3 and GLUT4 proteins were assessed by WB (n = 6). (d, e) Glucose uptake assay was performed by using 2-NBDG (n = 6, scale bar = 10 μm). (f, g) Immunofluorescence was used to detect the level of GLUT3 (cytoskeleton was labeled with β-tubulin) (n = 6, scale bar = 50 μm). (h, i) The expression of GLUT4 was detected by immunofluorescence (cytoskeleton was labeled with phalloidin) (n = 6, scale bar = 50 μm). Data are represented as the means ± SD. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001.

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