Product: PPP1R2 Antibody
Catalog: AF5455
Description: Rabbit polyclonal antibody to PPP1R2
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 23 kDa; 23kD(Calculated).
Uniprot: P41236
RRID: AB_2837939

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
PPP1R2 Antibody detects endogenous levels of total PPP1R2.
RRID:
AB_2837939
Cite Format: Affinity Biosciences Cat# AF5455, RRID:AB_2837939.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

IPP 2; IPP-2; IPP2; IPP2_HUMAN; MGC87148; Phosphatase inhibitor 2; Phosphoprotein phosphatase; Ppp1r2; PPP1R2 protein; Protein phosphatase 1 regulatory (inhibitor) subunit 2; Protein phosphatase 1 regulatory subunit 2; Protein phosphatase inhibitor 2;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Description:
Phosphorylation on Thr-73 by GSK3 activates PP1 by dissociating the PP1-PPP1R2 complex (By similarity). Phosphorylation on Ser-44 by ATM activates PP1 by dissociating the PP1-PPP1R2 complex.
Sequence:
MAASTASHRPIKGILKNKTSTTSSMVASAEQPRGNVDEELSKKSQKWDEMNILATYHPADKDYGLMKIDEPSTPYHSMMGDDEDACSDTEATEAMAPDILARKLAAAEGLEPKYRIQEQESSGEEDSDLSPEEREKKRQFEMKRKLHYNEGLNIKLARQLISKDLHDDDEDEEMLETADGESMNTEESNQGSTPSDQQQNKLRSS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Rabbit
100
Chicken
78
Horse
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P41236 As Substrate

Site PTM Type Enzyme
S4 Phosphorylation
S7 Phosphorylation
T19 Phosphorylation
S20 Phosphorylation
S23 Phosphorylation
S24 Phosphorylation
S28 Phosphorylation
R33 Methylation
S44 Phosphorylation Q13315 (ATM)
T55 Phosphorylation
Y56 Phosphorylation
Y63 Phosphorylation
S72 Phosphorylation P27448 (MARK3)
T73 Phosphorylation P49841 (GSK3B) , Q00535 (CDK5)
Y75 Phosphorylation
S77 Phosphorylation
S87 Phosphorylation P68400 (CSNK2A1) , P19784 (CSNK2A2) , O94921 (CDK14)
T89 Phosphorylation
T92 Phosphorylation
K103 Ubiquitination
K113 Ubiquitination
Y114 Phosphorylation
S121 Phosphorylation P68400 (CSNK2A1) , P19784 (CSNK2A2)
S122 Phosphorylation P68400 (CSNK2A1) , P19784 (CSNK2A2)
S127 Phosphorylation
S130 Phosphorylation
K145 Ubiquitination
Y148 Phosphorylation
K155 Ubiquitination
T193 Phosphorylation

Research Backgrounds

Function:

Inhibitor of protein-phosphatase 1.

PTMs:

Phosphorylation on Thr-73 by GSK3 activates PP1 by dissociating the PP1-PPP1R2 complex (By similarity). Phosphorylation on Ser-44 by ATM activates PP1 by dissociating the PP1-PPP1R2 complex.

Subunit Structure:

Heterodimer with PP1.

Family&Domains:

Belongs to the protein phosphatase inhibitor 2 family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.