5HT1A Receptor Antibody - #AF5453

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Product: 5HT1A Receptor Antibody
Catalog: AF5453
Description: Rabbit polyclonal antibody to 5HT1A Receptor
Application: WB IHC IF/ICC IHC-P
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 46 kDa; 46kD(Calculated).
Uniprot: P08908
RRID: AB_2837937

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC-p 1:50-1:200, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(82%), Bovine(91%), Horse(91%), Sheep(91%), Rabbit(100%), Dog(91%)
Clonality:
Polyclonal
Specificity:
5HT1A Receptor Antibody detects endogenous levels of total 5HT1A Receptor.
RRID:
AB_2837937
Cite Format: Affinity Biosciences Cat# AF5453, RRID:AB_2837937.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

5 HT 1A; 5 HT receptor 1A; 5 HT1A; 5 HT1A receptor; 5 hydroxytryptamine (serotonin) receptor 1A; 5 hydroxytryptamine (serotonin) receptor 1A G protein coupled; 5 hydroxytryptamine receptor 1A; 5-HT-1A; 5-HT1A; 5-hydroxytryptamine receptor 1A; 5HT 1A; 5HT 1A receptor; 5HT receptor 1A; 5HT1a; 5HT1A_HUMAN; ADRB2RL1; ADRBRL1; G 21; G protein coupled receptor; G-21; Gpcr 18; Gpcr18; Guanine nucleotide binding regulatory protein-coupled receptor; Htr1a; PFMCD; Serotonin 1A receptor; Serotonin 5HT1A receptor; Serotonin receptor 1A;

Immunogens

Immunogen:
Uniprot:

P08908(5HT1A_HUMAN) >>Visit HPA database.

Gene(ID):
HTR1A(3350)
Expression:
P08908 5HT1A_HUMAN:

Detected in lymph nodes, thymus and spleen. Detected in activated T-cells, but not in resting T-cells.

Description:
This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity.
Sequence:
MDVLSPGQGNNTTSPPAPFETGGNTTGISDVTVSYQVITSLLLGTLIFCAVLGNACVVAAIALERSLQNVANYLIGSLAVTDLMVSVLVLPMAALYQVLNKWTLGQVTCDLFIALDVLCCTSSILHLCAIALDRYWAITDPIDYVNKRTPRRAAALISLTWLIGFLISIPPMLGWRTPEDRSDPDACTISKDHGYTIYSTFGAFYIPLLLMLVLYGRIFRAARFRIRKTVKKVEKTGADTRHGASPAPQPKKSVNGESGSRNWRLGVESKAGGALCANGAVRQGDDGAALEVIEVHRVGNSKEHLPLPSEAGPTPCAPASFERKNERNAEAKRKMALARERKTVKTLGIIMGTFILCWLPFFIVALVLPFCESSCHMPTLLGAIINWLGYSNSLLNPVIYAYFNKDFQNAFKKIIKCKFCRQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Horse
91
Bovine
91
Sheep
91
Dog
91
Pig
82
Chicken
73
Xenopus
67
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P08908 As Substrate

Site PTM Type Enzyme
T196 Phosphorylation
S199 Phosphorylation
Y205 Phosphorylation
Y215 Phosphorylation
T240 Phosphorylation
K324 Acetylation
K334 Ubiquitination

Research Backgrounds

Function:

G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling inhibits adenylate cyclase activity and activates a phosphatidylinositol-calcium second messenger system that regulates the release of Ca(2+) ions from intracellular stores. Plays a role in the regulation of 5-hydroxytryptamine release and in the regulation of dopamine and 5-hydroxytryptamine metabolism. Plays a role in the regulation of dopamine and 5-hydroxytryptamine levels in the brain, and thereby affects neural activity, mood and behavior. Plays a role in the response to anxiogenic stimuli.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in lymph nodes, thymus and spleen. Detected in activated T-cells, but not in resting T-cells.

Subunit Structure:

Heterodimer; heterodimerizes with GPER1.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family. 5-hydroxytryptamine receptor subfamily. HTR1A sub-subfamily.

Research Fields

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Nervous system > Serotonergic synapse.

· Organismal Systems > Sensory system > Taste transduction.

References

1). Role of 5-HT1A receptor in insular cortex mediating stress – induced visceral sensory dysfunction. Neurogastroenterology & Motility (PubMed: 26969829) [IF=3.5]

Application: WB    Species:    Sample:

Figure 3 Comparison of the expression levels of HTR1As and NR2B in bilateral insular cortexes among three groups. The mRNA and protein expression levels of HTR1As (A and C) and NR2B (B and D) in bilateral insular cortexes were compared among WAS, sham WAS and normal control groups. (E) and (F): Representative immunoblots from the bilateral insular cortexes show the expression of NR2B, 5HTR1A and GAPDH, with an indication of size (kilodaltons, kDa). *represented that the difference was significant, p < 0.05. WAS, water avoidance stress.
2). Chemical Composition and Hypnotic Effect of Picea mariana Essential Oils. Journal of Essential Oil Bearing Plants [IF=2.4]
3). Antidepressant-Like Effect of Essential Oils From Citrus reticulata in Reserpine-Induced Depressive Mouse. Natural Product Communications [IF=1.8]

Application: IHC    Species: Mouse    Sample: cortex, hippocampus, and hypothalamus

Figure 3. Effects of CREOs on 5HT-1A and GR expressions in cortex, hippocampus, and hypothalamus. (A) Immunohistochemistry images of 5HT-1A (brown), scale bar = 50-μm. (B) Quantification analysis of 5HT-1A positive cells. (C) Immunohistochemistry images of GR (brown), scale bar = 50-μm. (D) Quantification analysis of GR-positive cells. Data were expressed as the Mean ± SD (n = 6). The significance of differences versus the Res group is at *P 

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