MMP8 Antibody - #AF5446
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Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF5446, RRID:AB_2837930.
Fold/Unfold
CLG 1; CLG1; Collagenase 1; Collagenase 1 neutrophil; HNC; Matrix metallopeptidase 8 (neutrophil collagenase); Matrix metalloprotease 8; Matrix metalloproteinase-8; MMP 8; MMP-8; Mmp8; MMP8_HUMAN; Neutrophil collagenase; PMNL CL; PMNL collagenase; PMNL-CL; PMNLCL;
Immunogens
- P22894 MMP8_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MFSLKTLPFLLLLHVQISKAFPVSSKEKNTKTVQDYLEKFYQLPSNQYQSTRKNGTNVIVEKLKEMQRFFGLNVTGKPNEETLDMMKKPRCGVPDSGGFMLTPGNPKWERTNLTYRIRNYTPQLSEAEVERAIKDAFELWSVASPLIFTRISQGEADINIAFYQRDHGDNSPFDGPNGILAHAFQPGQGIGGDAHFDAEETWTNTSANYNLFLVAAHEFGHSLGLAHSSDPGALMYPNYAFRETSNYSLPQDDIDGIQAIYGLSSNPIQPTGPSTPKPCDPSLTFDAITTLRGEILFFKDRYFWRRHPQLQRVEMNFISLFWPSLPTGIQAAYEDFDRDLIFLFKGNQYWALSGYDILQGYPKDISNYGFPSSVQAIDAAVFYRSKTYFFVNDQFWRYDNQRQFMEPGYPKSISGAFPGIESKVDAVFQQEHFFHVFSGPRYYAFDLIAQRVTRVARGNKWLNCRYG
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P22894 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T30 | Phosphorylation | Uniprot | |
| T32 | Phosphorylation | Uniprot | |
| Y36 | Phosphorylation | Uniprot | |
| Y383 | Phosphorylation | Uniprot | |
| S385 | Phosphorylation | Uniprot | |
| S422 | Phosphorylation | Uniprot |
Research Backgrounds
Can degrade fibrillar type I, II, and III collagens.
Cytoplasmic granule. Secreted>Extracellular space>Extracellular matrix.
Note: Stored in intracellular granules.
Neutrophils.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Belongs to the peptidase M10A family.
References
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