Phospho-MRLC1 (Ser19) Antibody | Affinity Biosciences

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Product:	Phospho-MRLC1 (Ser19) Antibody
Catalog:	AF5443
Description:	Rabbit polyclonal antibody to Phospho-MRLC1 (Ser19)
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	20 kDa; 20kD(Calculated).
Uniprot:	P24844
RRID:	AB_2837927

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

Phospho-MRLC1 (Ser19) Antibody detects endogenous levels of MRLC1 only when phosphorylated at Ser19.

RRID:

AB_2837927
Cite Format: Affinity Biosciences Cat# AF5443, RRID:AB_2837927.

Conjugate:

Unconjugated.

Purification:

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

20 kDa myosin light chain;Human 20kDa myosin light chain (MLC2) mRNA complete cds;LC20;MGC3505;MLC 2;MLC-2C;MLC2;MLY 9;MRLC1;MYL9;MYL9_HUMAN;Myosin light chain 9 regulatory;Myosin light polypeptide 9 regulatory;myosin regulatory light chain 1;Myosin regulatory light chain 2;Myosin regulatory light chain 2 smooth muscle isoform;Myosin regulatory light chain 9;Myosin regulatory light chain MRLC1;Myosin regulatory light polypeptide 9;Myosin RLC;Myosin vascular smooth muscle light chain 2;MYRL2;OTTHUMP00000030857;smooth muscle isoform;

Immunogens

Immunogen:

Uniprot:

P24844(MYL9_HUMAN) >>Visit HPA database.

Gene(ID):

MYL9(10398)

Expression:

P24844 MYL9_HUMAN:

Smooth muscle tissues and in some, but not all, nonmuscle cells.

Description:

Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion.

Sequence:

P24844 MYL9_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSSKRAKAKTTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLGKNPTDEYLEGMMSEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEASGFIHEDHLRELLTTMGDRFTDEEVDEMYREAPIDKKGNFNYVEFTRILKHGAKDKDD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Chicken

100

Rabbit

100

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P24844 As Substrate

P24844

Site	PTM Type	Enzyme	Source
S2	Phosphorylation	P17252 (PRKCA)	Uniprot
S3	Phosphorylation	P17252 (PRKCA)	Uniprot
T10	Phosphorylation	P17252 (PRKCA)	Uniprot
T11	Phosphorylation		Uniprot
T19	Phosphorylation	O43293 (DAPK3) , O14578 (CIT) , Q15746 (MYLK) , Q5VT25 (CDC42BPA) , Q13464 (ROCK1)	Uniprot
S20	Phosphorylation	O43293 (DAPK3) , Q5VT25 (CDC42BPA) , O75116 (ROCK2) , Q14012 (CAMK1) , Q15746 (MYLK) , Q13153 (PAK1) , Q13177 (PAK2) , Q9Y5S2 (CDC42BPB) , O14578 (CIT) , Q13464 (ROCK1)	Uniprot
S29	Phosphorylation		Uniprot
K51	Acetylation		Uniprot
K51	Ubiquitination		Uniprot
S60	Phosphorylation		Uniprot
T97	Phosphorylation		Uniprot
S115	Phosphorylation		Uniprot
T128	Phosphorylation		Uniprot
T135	Phosphorylation		Uniprot
Y143	Phosphorylation	P00533 (EGFR)	Uniprot
K150	Ubiquitination		Uniprot
K151	Sumoylation		Uniprot
K151	Ubiquitination		Uniprot
Y156	Phosphorylation	P00533 (EGFR)	Uniprot
K164	Ubiquitination		Uniprot
K168	Acetylation		Uniprot

Research Backgrounds

P24844_MYL9_HUMAN

Function:

PTMs:

Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge.

Tissue Specificity:

Smooth muscle tissues and in some, but not all, nonmuscle cells.

Subunit Structure:

Myosin is a hexamer of 2 heavy chains and 4 light chains: interacts with myosin heavy chain MYO19.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction. (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

References

1). A comparative study of the ameliorative effects of hyaluronic acid oligosaccharides and hyaluronic acid on DSS-induced colitis in mice and research on relevant mechanisms. Food & Function, 2023 [IF=6.1]

2). Methamphetamine reduces expressions of tight junction proteins, rearranges F-actin cytoskeleton and increases the blood brain barrier permeability via the RhoA/ROCK-dependent pathway. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2019 (PubMed: 30594393) [IF=3.1]

Application: WB Species: rat Sample: hippocampus

Fig. 1.| Western blotting analysis for proteins of RhoA, ROCK, MLC, cofilin, p-MLC, p-cofilin,MMP-9, occludin, claudin-5 and ZO-1 in rat hippocampus. Compared with the control,over-expressions of RhoA, ROCK, MLC, cofilin, p-MLC, p-cofilin and MMP-9 were observed in the METH-treated rats, while expressions of TJ proteins of occludin, claudin-5 and ZO-1 significantly decreased. Results were quantified by densitometry and normalized to b-actin expression. IDV is integrated density value. Significant compared with the control(*p < 0.05, **p < 0.01).

3). GLP-1 attenuates Ang II-induced proliferation and migration in rat aorta smooth muscle cells via inhibition of the RhoA/ROCK2 signaling pathway. PHARMAZIE, 2018 (PubMed: 30522551) [IF=1.6]

Application: WB Species: rat Sample: RASMCs

Fig. 5: |Effects of GLP-1, Ex-4, forskolin, Y-27632, and H89 on Ang II-induced RASMC migration. (A and E) Cell migration was measured by the wound-healing assay. (C and G) Protein expression level of p-MLC was evaluated by Western blot. Data are presented as means ± SD (n = 3,* P < 0.05 vs. control, # P < 0.05 vs. Ang II, & P < 0.05 vs. Ang II+GLP-1 or Ang II+Ex-4).

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For Research Use Only.
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Phospho-MRLC1 (Ser19) Antibody - #AF5443