Phospho-ATRIP (Ser224) Antibody - #AF5437

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Product: Phospho-ATRIP (Ser224) Antibody
Catalog: AF5437
Description: Rabbit polyclonal antibody to Phospho-ATRIP (Ser224)
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Sheep, Rabbit
Mol.Wt.: 85 kDa; 86kD(Calculated).
Uniprot: Q8WXE1
RRID: AB_2837921

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Sheep(86%), Rabbit(88%)
Clonality:
Polyclonal
Specificity:
Phospho-ATRIP (Ser224) Antibody detects endogenous levels of ATRIP only when phosphorylated at Ser224.
RRID:
AB_2837921
Cite Format: Affinity Biosciences Cat# AF5437, RRID:AB_2837921.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AGS1; ATIP; ATM and Rad3 related interacting protein; ATM and Rad3-related-interacting protein; ATR interacting protein; ATR-interacting protein; Atrip; ATRIP_HUMAN; DKFZp762J2115; FLJ12343; MGC20625; MGC21482; MGC26740;

Immunogens

Immunogen:
Uniprot:

Q8WXE1(ATRIP_HUMAN) >>Visit HPA database.

Gene(ID):
ATRIP(84126)
Expression:
Q8WXE1 ATRIP_HUMAN:

Ubiquitous.

Description:
Required for checkpoint signaling after DNA damage. Required for ATR expression, possibly by stabilizing the protein.
Sequence:
MAGTSAPGSKRRSEPPAPRPGPPPGTGHPPSKRARGFSAAAAPDPDDPFGAHGDFTADDLEELDTLASQALSQCPAAARDVSSDHKVHRLLDGMSKNPSGKNRETVPIKDNFELEVLQAQYKELKEKMKVMEEEVLIKNGEIKILRDSLHQTESVLEEQRRSHFLLEQEKTQALSDKEKEFSKKLQSLQSELQFKDAEMNELRTKLQTSERANKLAAPSVSHVSPRKNPSVVIKPEACSPQFGKTSFPTKESFSANMSLPHPCQTESGYKPLVGREDSKPHSLRGDSIKQEEAQKSFVDSWRQRSNTQGSILINLLLKQPLIPGSSLSLCHLLSSSSESPAGTPLQPPGFGSTLAGMSGLRTTGSYDGSFSLSALREAQNLAFTGLNLVARNECSRDGDPAEGGRRAFPLCQLPGAVHFLPLVQFFIGLHCQALQDLAAAKRSGAPGDSPTHSSCVSSGVETNPEDSVCILEGFSVTALSILQHLVCHSGAVVSLLLSGVGADSAAGEGNRSLVHRLSDGDMTSALRGVADDQGQHPLLKMLLHLLAFSSAATGHLQASVLTQCLKVLVKLAENTSCDFLPRFQCVFQVLPKCLSPETPLPSVLLAVELLSLLADHDQLAPQLCSHSEGCLLLLLYMYITSRPDRVALETQWLQLEQEVVWLLAKLGVQSPLPPVTGSNCQCNVEVVRALTVMLHRQWLTVRRAGGPPRTDQQRRTVRCLRDTVLLLHGLSQKDKLFMMHCVEVLHQFDQVMPGVSMLIRGLPDVTDCEEAALDDLCAAETDVEDPEVECG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Rabbit
88
Sheep
86
Pig
75
Dog
63
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8WXE1 As Substrate

Site PTM Type Enzyme
S9 Phosphorylation
S13 Phosphorylation
T26 Phosphorylation
S31 Phosphorylation
K32 Acetylation
S38 Phosphorylation
S68 Phosphorylation Q13535 (ATR)
S72 Phosphorylation Q13535 (ATR)
K96 Ubiquitination
K122 Ubiquitination
S162 Phosphorylation
K205 Ubiquitination
K214 Ubiquitination
S219 Phosphorylation
S221 Phosphorylation
S224 Phosphorylation P24941 (CDK2)
S239 Phosphorylation P24941 (CDK2)
K270 Ubiquitination
K295 Ubiquitination
S305 Phosphorylation
T307 Phosphorylation
S310 Phosphorylation
S512 Phosphorylation
S518 Phosphorylation
T523 Phosphorylation
Y636 Phosphorylation

Research Backgrounds

Function:

Required for checkpoint signaling after DNA damage. Required for ATR expression, possibly by stabilizing the protein.

PTMs:

Phosphorylated by ATR.

Subcellular Location:

Nucleus.
Note: Redistributes to discrete nuclear foci upon DNA damage.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Interacts with ATR (By similarity). Heterodimer with ATR. The heterodimer binds the RPA complex and is then recruited to single-stranded DNA. Interacts with CEP164 (via N-terminus). Interacts with CINP.

Family&Domains:

The EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage.

Belongs to the ATRIP family.

Research Fields

· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.

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