Phospho-eIF4B (Ser422) Antibody - #AF5428

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Product: Phospho-eIF4B (Ser422) Antibody
Catalog: AF5428
Description: Rabbit polyclonal antibody to Phospho-eIF4B (Ser422)
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 69 kDa; 69kD(Calculated).
Uniprot: P23588
RRID: AB_2837912

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Phospho-eIF4B (Ser422) Antibody detects endogenous levels of eIF4B only when phosphorylated at Ser422.
RRID:
AB_2837912
Cite Format: Affinity Biosciences Cat# AF5428, RRID:AB_2837912.
Conjugate:
Unconjugated.
Purification:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

2310046H11Rik; AL024095; C85189; EIF 4B; eIF-4B; EIF4B; Eukaryotic initiation factor 4B; Eukaryotic translation initiation factor 4B; IF4B_HUMAN; PRO1843;

Immunogens

Immunogen:
Uniprot:

P23588(IF4B_HUMAN) >>Visit HPA database.

Gene(ID):
EIF4B(1975)
Description:
Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.
Sequence:
MAASAKKKNKKGKTISLTDFLAEDGGTGGGSTYVSKPVSWADETDDLEGDVSTTWHSNDDDVYRAPPIDRSILPTAPRAAREPNIDRSRLPKSPPYTAFLGNLPYDVTEESIKEFFRGLNISAVRLPREPSNPERLKGFGYAEFEDLDSLLSALSLNEESLGNRRIRVDVADQAQDKDRDDRSFGRDRNRDSDKTDTDWRARPATDSFDDYPPRRGDDSFGDKYRDRYDSDRYRDGYRDGYRDGPRRDMDRYGGRDRYDDRGSRDYDRGYDSRIGSGRRAFGSGYRRDDDYRGGGDRYEDRYDRRDDRSWSSRDDYSRDDYRRDDRGPPQRPKLNLKPRSTPKEDDSSASTSQSTRAASIFGGAKPVDTAAREREVEERLQKEQEKLQRQLDEPKLERRPRERHPSWRSEETQERERSRTGSESSQTGTSTTSSRNARRRESEKSLENETLNKEEDCHSPTSKPPKPDQPLKVMPAPPPKENAWVKRSSNPPARSQSSDTEQQSPTSGGGKVAPAQPSEEGPGRKDENKVDGMNAPKGQTGNSSRGPGDGGNRDHWKESDRKDGKKDQDSRSAPEPKKPEENPASKFSSASKYAALSVDGEDENEGEDYAE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Pig
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P23588 As Substrate

Site PTM Type Enzyme
K6 Acetylation
S16 Phosphorylation
Y33 Phosphorylation
S39 Phosphorylation
S52 Phosphorylation
S57 Phosphorylation
Y63 Phosphorylation
R70 Methylation
S71 Phosphorylation
T75 Phosphorylation
R78 Methylation
K92 Ubiquitination
S93 Phosphorylation
Y96 Phosphorylation
T97 Phosphorylation
Y105 Phosphorylation
S111 Phosphorylation
R117 Methylation
S131 Phosphorylation
Y141 Phosphorylation
S155 Phosphorylation
S160 Phosphorylation
K177 Ubiquitination
R182 Methylation
S183 Phosphorylation
S192 Phosphorylation
T195 Phosphorylation
T197 Phosphorylation
T205 Phosphorylation
S207 Phosphorylation
Y211 Phosphorylation
S219 Phosphorylation
K223 Ubiquitination
Y224 Phosphorylation
Y228 Phosphorylation
S230 Phosphorylation
Y233 Phosphorylation
Y237 Phosphorylation
Y241 Phosphorylation
Y252 Phosphorylation
Y258 Phosphorylation
R261 Methylation
S263 Phosphorylation
Y266 Phosphorylation
R268 Methylation
Y270 Phosphorylation
S272 Phosphorylation
R273 Methylation
R279 Methylation
S283 Phosphorylation
Y285 Phosphorylation
Y291 Phosphorylation
R292 Methylation
R297 Methylation
Y298 Phosphorylation
Y302 Phosphorylation
R308 Methylation
S309 Phosphorylation
S311 Phosphorylation
S312 Phosphorylation
Y316 Phosphorylation
S317 Phosphorylation
Y321 Phosphorylation
K333 Sumoylation
S340 Phosphorylation
T341 Phosphorylation
S347 Phosphorylation
S348 Phosphorylation
S350 Phosphorylation
T351 Phosphorylation
S352 Phosphorylation
S354 Phosphorylation
S359 Phosphorylation
K365 Acetylation
K365 Ubiquitination
T369 Phosphorylation
S406 Phosphorylation Q9P1W9 (PIM2) , Q14680 (MELK)
S409 Phosphorylation
T412 Phosphorylation
S418 Phosphorylation
T420 Phosphorylation
S422 Phosphorylation P51812 (RPS6KA3) , Q15418 (RPS6KA1) , P23443 (RPS6KB1) , P31749 (AKT1)
S424 Phosphorylation
S425 Phosphorylation
T427 Phosphorylation
T429 Phosphorylation
S430 Phosphorylation
T431 Phosphorylation
T432 Phosphorylation
S433 Phosphorylation
S434 Phosphorylation
S442 Phosphorylation
K444 Acetylation
S445 Phosphorylation
T450 Phosphorylation
S459 Phosphorylation
T461 Phosphorylation
S462 Phosphorylation
K463 Ubiquitination
K486 Methylation
S488 Phosphorylation
S489 Phosphorylation
S495 Phosphorylation
S497 Phosphorylation
S498 Phosphorylation
T500 Phosphorylation
S504 Phosphorylation
T506 Phosphorylation
S518 Phosphorylation
K537 Ubiquitination
S585 Phosphorylation
K586 Acetylation
K586 Ubiquitination
S589 Phosphorylation
S591 Phosphorylation
K592 Ubiquitination
Y593 Phosphorylation
S597 Phosphorylation
Y609 Phosphorylation

Research Backgrounds

Function:

Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.

PTMs:

Phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1; phosphorylation enhances the affinity of EIF4B for the EIF3 complex.

Subunit Structure:

Self-associates and interacts with EIF3 p170 subunit.

Research Fields

· Environmental Information Processing > Signal transduction > mTOR signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Genetic Information Processing > Translation > RNA transport.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

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