hnRNP K Antibody | Affinity Biosciences

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Western blot analysis of hnRNP K expression in LOVO cells.

Product:	hnRNP K Antibody
Catalog:	AF5422
Description:	Rabbit polyclonal antibody to hnRNP K
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	51 kDa; 51kD(Calculated).
Uniprot:	P61978
RRID:	AB_2837906

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(100%), Zebrafish(89%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

hnRNP K Antibody detects endogenous levels of total hnRNP K.

RRID:

AB_2837906
Cite Format: Affinity Biosciences Cat# AF5422, RRID:AB_2837906.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CSBP; dC stretch binding protein; FLJ41122; Heterogeneous nuclear ribonucleoprotein K; hnRNP K; HNRNPK; HNRPK; HNRPK_HUMAN; Transformation up regulated nuclear protein; Transformation up-regulated nuclear protein; Transformation upregulated nuclear protein; TUNP;

Immunogens

Immunogen:

Uniprot:

P61978(HNRPK_HUMAN) >>Visit HPA database.

Gene(ID):

HNRNPK(3190)

Description:

Sequence:

P61978 HNRPK_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

METEQPEETFPNTETNGEFGKRPAEDMEEEQAFKRSRNTDEMVELRILLQSKNAGAVIGKGGKNIKALRTDYNASVSVPDSSGPERILSISADIETIGEILKKIIPTLEEGLQLPSPTATSQLPLESDAVECLNYQHYKGSDFDCELRLLIHQSLAGGIIGVKGAKIKELRENTQTTIKLFQECCPHSTDRVVLIGGKPDRVVECIKIILDLISESPIKGRAQPYDPNFYDETYDYGGFTMMFDDRRGRPVGFPMRGRGGFDRMPPGRGGRPMPPSRRDYDDMSPRRGPPPPPPGRGGRGGSRARNLPLPPPPPPRGGDLMAYDRRGRPGDRYDGMVGFSADETWDSAIDTWSPSEWQMAYEPQGGSGYDYSYAGGRGSYGDLGGPIITTQVTIPKDLAGSIIGKGGQRIKQIRHESGASIKIDEPLEGSEDRIITITGTQDQIQNAQYLLQNSVKQYSGKFF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Chicken

100

Rabbit

100

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P61978 As Substrate

P61978

Site	PTM Type	Enzyme	Source
M1	Acetylation		Uniprot
T3	Phosphorylation		Uniprot
K21	Acetylation		Uniprot
R22	Methylation		Uniprot
K34	Acetylation		Uniprot
K34	Methylation		Uniprot
K34	Sumoylation		Uniprot
K34	Ubiquitination		Uniprot
S36	Phosphorylation		Uniprot
T39	Phosphorylation		Uniprot
R46	Methylation		Uniprot
K52	Sumoylation		Uniprot
K52	Ubiquitination		Uniprot
K60	Acetylation		Uniprot
K60	Sumoylation		Uniprot
K60	Ubiquitination		Uniprot
K63	Ubiquitination		Uniprot
K66	Ubiquitination		Uniprot
Y72	Phosphorylation	P12931 (SRC)	Uniprot
S75	Phosphorylation		Uniprot
S77	Phosphorylation		Uniprot
S81	Phosphorylation		Uniprot
S82	Phosphorylation		Uniprot
S89	Phosphorylation		Uniprot
K102	Ubiquitination		Uniprot
K103	Ubiquitination		Uniprot
S116	Phosphorylation		Uniprot
T118	Phosphorylation		Uniprot
T120	Phosphorylation		Uniprot
S121	Phosphorylation		Uniprot
Y135	Phosphorylation		Uniprot
Y138	Phosphorylation		Uniprot
K139	Methylation		Uniprot
K139	Ubiquitination		Uniprot
S141	Phosphorylation		Uniprot
R148	Methylation		Uniprot
S154	Phosphorylation		Uniprot
K163	Sumoylation		Uniprot
K163	Ubiquitination		Uniprot
K166	Ubiquitination		Uniprot
K168	Ubiquitination		Uniprot
K179	Acetylation		Uniprot
K179	Ubiquitination		Uniprot
C184	S-Nitrosylation		Uniprot
C185	S-Nitrosylation		Uniprot
S188	Phosphorylation		Uniprot
R191	Methylation		Uniprot
K198	Methylation		Uniprot
K198	Ubiquitination		Uniprot
R201	Methylation		Uniprot
K207	Ubiquitination		Uniprot
S214	Phosphorylation		Uniprot
S216	Phosphorylation	P53779 (MAPK10) , P06493 (CDK1) , P24941 (CDK2) , P45983 (MAPK8)	Uniprot
K219	Acetylation		Uniprot
K219	Sumoylation		Uniprot
K219	Ubiquitination		Uniprot
Y225	Phosphorylation	P12931 (SRC)	Uniprot
Y230	Phosphorylation	P12931 (SRC)	Uniprot
Y234	Phosphorylation	P12931 (SRC)	Uniprot
Y236	Phosphorylation	P12931 (SRC)	Uniprot
R246	Methylation		Uniprot
R247	Methylation		Uniprot
R249	Methylation		Uniprot
R256	Methylation		Uniprot
R258	Methylation		Uniprot
R263	Methylation		Uniprot
R268	Methylation		Uniprot
R271	Methylation		Uniprot
S276	Phosphorylation		Uniprot
R277	Methylation		Uniprot
R278	Methylation		Uniprot
Y280	Phosphorylation		Uniprot
S284	Phosphorylation	P28482 (MAPK1) , P27361 (MAPK3)	Uniprot
R286	Methylation		Uniprot
R296	Methylation		Uniprot
R299	Methylation		Uniprot
S302	Phosphorylation	Q05655 (PRKCD)	Uniprot
R303	Methylation		Uniprot
R305	Methylation		Uniprot
R316	Methylation		Uniprot
Y323	Phosphorylation		Uniprot
R325	Methylation		Uniprot
R326	Methylation		Uniprot
R328	Methylation		Uniprot
Y333	Phosphorylation		Uniprot
S353	Phosphorylation	P53779 (MAPK10) , P45983 (MAPK8) , P27361 (MAPK3)	Uniprot
Y361	Phosphorylation		Uniprot
Y369	Phosphorylation		Uniprot
Y371	Phosphorylation		Uniprot
S372	Phosphorylation		Uniprot
Y373	Phosphorylation		Uniprot
R377	Methylation		Uniprot
S379	Phosphorylation	O14965 (AURKA)	Uniprot
Y380	Phosphorylation	P12931 (SRC)	Uniprot
T389	Phosphorylation		Uniprot
T390	Phosphorylation		Uniprot
T393	Phosphorylation		Uniprot
K396	Ubiquitination		Uniprot
S401	Phosphorylation		Uniprot
K405	Sumoylation		Uniprot
K405	Ubiquitination		Uniprot
S417	Phosphorylation		Uniprot
S420	Phosphorylation		Uniprot
K422	Acetylation		Uniprot
K422	Sumoylation		Uniprot
K422	Ubiquitination		Uniprot
S430	Phosphorylation		Uniprot
R433	Methylation		Uniprot
T436	Phosphorylation		Uniprot
T440	Phosphorylation		Uniprot
Y449	Phosphorylation		Uniprot
S454	Phosphorylation		Uniprot
K456	Ubiquitination		Uniprot
Y458	Phosphorylation	P12931 (SRC)	Uniprot
K461	Acetylation		Uniprot
K461	Ubiquitination		Uniprot

Research Backgrounds

P61978_HNRPK_HUMAN

Function:

One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction (By similarity). As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21 (lincRNA-p21), a non-coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest.

PTMs:

Arg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine.

Sumoylated by CBX4. Sumoylation is increased upon DNA damage, such as that produced by doxorubicin, etoposide, UV light and camptothecin, due to enhanced CBX4 phosphorylation by HIPK2 under these conditions.

Ubiquitinated by MDM2. Doxorubicin treatment does not affect monoubiquitination, but slightly decreases HNRNPK poly-ubiquitination.

O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.

Subcellular Location:

Cytoplasm. Nucleus>Nucleoplasm. Cell projection>Podosome.
Note: Recruited to p53/TP53-responsive promoters, in the presence of functional p53/TP53 (PubMed:16360036). In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear (PubMed:18775702).

Subunit Structure:

Interacts with RBM42 and ZIK1 (By similarity). Interacts with BRDT (By similarity). Identified in the spliceosome C complex. Interacts with ANKRD28. Interacts with ASFV p30 protein. Interacts with DDX1. Interacts with MDM2; this interaction leads to ubiquitination and proteasomal degradation. Interacts with p53/TP53. Interacts with IVNS1ABP.

(Microbial infection) Interacts with HCV core protein.

Research Fields

· Genetic Information Processing > Transcription > Spliceosome.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

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hnRNP K Antibody - #AF5422