Caspase 1 Antibody | Affinity Biosciences

WB
IHC
Cites

1/70

Western blot analysis of Caspase 1 expression in 293 cells.

AF5418 at 1/100 staining Mouse spleen tissue by IHC-P.

AF5418 at 1/100 staining Mouse lung tissue by IHC-P.

AF5418 at 1/100 staining Rat spleen tissue by IHC-P.

$Fig.2 |NaAsO2 induced autophagy, activation of NLRP3 inflammasome and hepatic stellate cell in vivo. (A) The expression level of LC3 and p62 in rat liver following exposure to NaAsO2. The protein fraction was analyzed by Western blot. (B)The expression level of cytoplasmic CTSB in rat livers following exposure to NaAsO2.The protein fraction was analyzed by Western blot. (C) The expression level of NLRP3 inflammasome-associated proteins in rat livers following exposure to NaAsO2.The protein fraction was analyzed by Western blot.$

Fig.

$Fig.2 NaAsO2 induced autophagy, activation of NLRP3 inflammasome and 662 hepatic stellate cell in vivo. (A) The expression level of LC3 and p62 in rat livers 663 following exposure to NaAsO2. The protein fraction was analyzed by Western blot. (B) 664 The expression level of cytoplasmic CTSB in rat livers following exposure to NaAsO2. 665 The protein fraction was analyzed by Western blot. (C) The expression level of NLRP3 inflammasome-associated proteins in rat livers following exposure to NaAsO2. 667 The protein fraction was analyzed by Western blot. (D) The expression level of 668 Collagen- , α-SMA, MMP-1, and TIMP-1in rat livers following exposure to NaAsO2. 669 The protein fraction was analyzed by Western blot. (E) α-SMA was detected by IF. 670 White arrows indicate positive staining. Values are mean ± SD, and n = 5. *p < 0.05, 671 **p < 0.01 compared with the control group; Scale bar = 100 µm$

Fig.

$Fig.3 NaAsO2 impairs insulin signaling via activation of NLRP3 inflammasome in HepG2 cells. HepG2 cells were pretreated with 1 μg/ml LPS for 4 hours, 5 μM MCC950, for 4 hours, and were then treated with 4 μM NaAsO2 for 24 hours. Cytosolic fractions were analyzed by Western blot analysis. GAPDH was used as an internal control. The efficiency of MCC950, and its effect on NLRP3, caspase-1 activation, IL-1β and IL- 18 production in NaAsO2-treated HepG2 cells (A-E). HepG2 cells were stimulated with 100 nM insulin for 10 min at the end of treatment. Cytosolic fractions were analyzed by Western blot analysis. GAPDH was used as an internal control. The efficiency of MCC950, and its effect on p-IRS (Ser 307)/IRS1 and p-AKT (Ser473)/AKT1 ratio in NaAsO2-treated HepG2 cells (F-H). Insulin‐ stimulated glucose uptake in HepG2 cells was measured using a glucose assay kit (I). Results are mean ± SEM (n = 3). *P < 0.05 compare with the LPS group. #P<0.05 compare with 4 μM NaAsO2 group.$

Fig.

Figure 4.

Fig.

FIGURE 8| Effect of calycosin on the expression of proteins associated with the AMPK/SKP2/CARM1 signalling pathway in TNF-αinduced C2C12 cells in vitro.

Fig.

Figure 5 The expression levels of p-P38, P38, p-IκBα, caspase 1, COX2, and iNOS proteins in AD + NS, AD + donepezil, and AD + BM25 groups.

Fig.

FIGURE 9 Effects of OCOP and COP on the NLRP3 inflammasome in DSS-induced mice (A) Representative Western blotting images of NLRP3, ASC, Caspase-1and Caspase-1 p10 (B–E) The protein expression levels of NLRP3, ASC, Caspase-1 and Caspase-1 p10 in the NLRP3 inflammasome.

Figure 4 Effects of Rg1 on the expression levels of NLRP1, cleaved-caspase-1, IL-1β, NF-κB and p-NF-κB in LPS-induced HT22 cells determined by western blotting.

FIGURE 7 Effect of DHB on NLRP3 inflammasome signaling in pO/HX-induced hyperuricemic mice.

Figure 6: |MEL attenuated the protein levels of NLRP3, TLR4, NF-κB, and caspase-1 of the terminal ileum tissues in NEC mice.

Fig.

Figure 6.

Figure 6 |C-PC treatment suppresses the expression of NLRP3 inflammasome and NF-κB signaling pathway in lung tissues.

FIGURE 1 |Effects of combined administration of RvE1 and LXA4 on pro-inflammatory factor expression.

FIGURE s4 |The total proteins of infected primary peritoneal macrophages were extracted at 24, 36, 48, and 72 h post-infection and then subjected for Western blotting.

Figure 4.

Figure 6 Effect of HPC on the expression of caspase-1, GSDMD, and NLRP3 in the brains of cerebral I/R injury model mice.

Figure 7.

Figure 5.

Fig.

Figure 5 Effects of PAL and Col (1 µM) on NF-κB/NLRP3 and Nrf2 signaling pathways in LPS plus MSU-activated THP-1 cells.

Figure 3 (a) Immunofluorescent staining labeled pyroptosis marker caspase-1 was conducted to assess the extent of pyroptosis in each group of mice (scale bar = 50 μm); (b) Western blot was used to examine the expression of pyroptosis markers in the muscle homogenate of each group of mice.

Fig.

FIGURE 10 Effect of TTWC on the Level of NLRP3, ASC, pro-caspase-1, caspase-1, pro-IL-1beta, IL-1beta, pro-IL-18 and IL-18 protein in the prefrontal cortex (B1-B8) (Mean ± SD, n = 3).

Figure 5 Milrinone diminishes the secretion of inflammatory cytokines and inhibits the formation of NLRP3 inflammasomes.

Figure 6 Tuina treatment influenced the expression of pyroptosis-related molecules in the hippocampi of HIE rats.

Figure 4 Hypoxia induces fibroblast-like synoviocytes (FLS) pyroptosis by upregulating the expression of nucleotide-binding oligomerization segment-like receptor family 3 (NLRP3) inflammasome in FLS and the effect of monomeric derivatives of paeoniflorin (MDP).

Figure 4 Quercetin alleviated ethanol-induced pyroptosis via the nuclear transfer of PGC1α.

Figure 6 PSG suppressed pyroptosis of the liver tissues in IBD mice.

FIGURE 4 Caspase-1–dependent canonical pathway activated RTEC pyroptosis.

Figure 5.

Figure 9 The expression of caspase 1 and caspase 3 in lung tissues was detected by immunohistochemistry.

Figure 10 The expressions of cleaved caspase 1/caspase 1 and cleaved caspase 3/caspase 3 in lung tissues were detected by western blot.

Figure 4 mRNA and protein expression levels of NLRP3 inflammasome.

Figure 8.

Figure 5 Effects of SeNPs on the protein expression of jejunal Casepase-1.

Figure 3 Fig.

FIGURE 2 PTS reduces reduction of NLRP3 inflammasome and apoptosis in DOX-treated mice.

Figure 5 Effect of DF supplementation on the activation of NLRP3 inflammasome.

FIGURE 8 CGGE inhibited NLRP3 Inflammasome in ipsilateral cortex.

图 6 柴胡皂苷D对免疫性肝纤维化大鼠肝脏组织中NLRP3炎症小体和细胞焦亡蛋白的影响(n=8，x±s).

FIGURE 2 PTS reduces reduction of NLRP3 inflammasome and apoptosis in DOX-treated mice.

expression in lung cancer.

Figure 1 Expression of NLRP3, Caspase-1, and GSDMD in the decidua of pregnant rats in each group.

Fig.

FIGURE 2 Effects of AB4 treatment on inflammatory infiltration and protein levels of IL‐1β, GFAP, NLRP3 and caspase‐1 in the spinal cord of the control, CIA and CIA + AB4 groups.

FIGURE 2 PTS reduces reduction of NLRP3 inflammasome and apoptosis in DOX-treated mice.

Figure 5 Que induces GSDMD-mediated pyroptosis in colon cancer cells through NLRP3 inflammasome activation.

Figure 5 BA suppresses pyroptosis and promotes anti-oxidation in the spinal cord of SCI rats.

Figure 4 Effects of AST and CY-09 on NLRP3, caspase-1 expression in 661W and ARPE-19 cells A: The mRNA levels of NLRP3 and caspase-1 in 661W cells; B: The protein levels of NLRP3 and cleaved caspase-1 in 661W and ARPE-19 cells.

FIGURE 2 PTS reduces reduction of NLRP3 inflammasome and apoptosis in DOX-treated mice.

Fig.

Product:	Caspase 1 Antibody
Catalog:	AF5418
Description:	Rabbit polyclonal antibody to Caspase 1
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Rabbit
Mol.Wt.:	45kDa(Pro); 45kD(Calculated).
Uniprot:	P29466
RRID:	AB_2837902

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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MS Report

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COA

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WB Handbook

IHC Protocol

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Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(89%), Rabbit(89%)

Clonality:

Polyclonal

Specificity:

Caspase 1 Antibody detects endogenous levels of total Caspase 1.

RRID:

AB_2837902
Cite Format: Affinity Biosciences Cat# AF5418, RRID:AB_2837902.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CASP-1; CASP1; CASP1_HUMAN; Caspase 1; Caspase-1 subunit p10; ICE; IL-1 beta-converting enzyme; IL-1BC; IL1 beta converting enzyme; IL1B convertase; Interleukin 1 beta convertase; Interleukin 1B converting enzyme; Interleukin-1 beta convertase; Interleukin-1 beta-converting enzyme; p45;

Immunogens

Immunogen:

Uniprot:

P29466(CASP1_HUMAN) >>Visit HPA database.

Gene(ID):

CASP1(834)

Expression:

P29466 CASP1_HUMAN:

Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.

Description:

Sequence:

P29466 CASP1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MADKVLKEKRKLFIRSMGEGTINGLLDELLQTRVLNKEEMEKVKRENATVMDKTRALIDSVIPKGAQACQICITYICEEDSYLAGTLGLSADQTSGNYLNMQDSQGVLSSFPAPQAVQDNPAMPTSSGSEGNVKLCSLEEAQRIWKQKSAEIYPIMDKSSRTRLALIICNEEFDSIPRRTGAEVDITGMTMLLQNLGYSVDVKKNLTASDMTTELEAFAHRPEHKTSDSTFLVFMSHGIREGICGKKHSEQVPDILQLNAIFNMLNTKNCPSLKDKPKVIIIQACRGDSPGVVWFKDSVGVSGNLSLPTTEEFEDDAIKKAHIEKDFIAFCSSTPDNVSWRHPTMGSVFIGRLIEHMQEYACSCDVEEIFRKVRFSFEQPDGRAQMPTTERVTLTRCFYLFPGH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

Rabbit

Horse

Sheep

Dog

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P29466 As Substrate

P29466

Site	PTM Type	Source
S16	Phosphorylation	Uniprot
T21	Phosphorylation	Uniprot
T32	Phosphorylation	Uniprot
K37	Ubiquitination	Uniprot
K44	Ubiquitination	Uniprot
T49	Phosphorylation	Uniprot
K53	Ubiquitination	Uniprot
K134	Ubiquitination	Uniprot
K148	Ubiquitination	Uniprot
S149	Phosphorylation	Uniprot
K158	Ubiquitination	Uniprot
K204	Ubiquitination	Uniprot
T226	Phosphorylation	Uniprot
S227	Phosphorylation	Uniprot
K268	Ubiquitination	Uniprot
K274	Ubiquitination	Uniprot
K278	Ubiquitination	Uniprot
S306	Phosphorylation	Uniprot
K319	Ubiquitination	Uniprot
K320	Ubiquitination	Uniprot
K325	Ubiquitination	Uniprot
S376	Phosphorylation	Uniprot

Research Backgrounds

P29466_CASP1_HUMAN

Function:

Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive. In apoptotic cells, cleaves SPHK2 which is released from cells and remains enzymatically active extracellularly.

PTMs:

The two subunits are derived from the precursor sequence by an autocatalytic mechanism.

Subcellular Location:

Cytoplasm. Cell membrane.

Tissue Specificity:

Subunit Structure:

Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The p20 subunit can also form a heterodimer with the epsilon isoform which then has an inhibitory effect. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NALP2 and whose function would be the activation of proinflammatory caspases. Both the p10 and p20 subunits interact with MEFV. Interacts with CARD17/INCA and CARD18. Interacts with SERPINB1; this interaction regulates CASP1 activity.

Family&Domains:

Belongs to the peptidase C14A family.

Research Fields

· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)

· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Human Diseases > Infectious diseases: Bacterial > Legionellosis.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)

· Organismal Systems > Immune system > Cytosolic DNA-sensing pathway. (View pathway)

References

1). Tetrahedral Framework Nucleic Acids Based Small Interfering RNA Targeting Receptor for Advanced Glycation End Products for Diabetic Complications Treatment. ACS Nano, 2023 (PubMed: 37751401) [IF=17.1]

2). Arsenic induces hepatic insulin resistance via mtROS-NLRP3 inflammasome pathway. JOURNAL OF HAZARDOUS MATERIALS, 2020 (PubMed: 32544768) [IF=13.6]

Application: WB Species: Human Sample: HepG2 cells

Fig.3 NaAsO2 impairs insulin signaling via activation of NLRP3 inflammasome in HepG2 cells. HepG2 cells were pretreated with 1 μg/ml LPS for 4 hours, 5 μM MCC950, for 4 hours, and were then treated with 4 μM NaAsO2 for 24 hours. Cytosolic fractions were analyzed by Western blot analysis. GAPDH was used as an internal control. The efficiency of MCC950, and its effect on NLRP3, caspase-1 activation, IL-1β and IL- 18 production in NaAsO2-treated HepG2 cells (A-E). HepG2 cells were stimulated with 100 nM insulin for 10 min at the end of treatment. Cytosolic fractions were analyzed by Western blot analysis. GAPDH was used as an internal control. The efficiency of MCC950, and its effect on p-IRS (Ser 307)/IRS1 and p-AKT (Ser473)/AKT1 ratio in NaAsO2-treated HepG2 cells (F-H). Insulin‐ stimulated glucose uptake in HepG2 cells was measured using a glucose assay kit (I). Results are mean ± SEM (n = 3). *P < 0.05 compare with the LPS group. #P<0.05 compare with 4 μM NaAsO2 group.

3). Water-extracted Lonicera japonica polysaccharide attenuates allergic rhinitis by regulating NLRP3-IL-17 signaling axis. Carbohydrate Polymers, 2022 (PubMed: 36184153) [IF=11.2]

4). Lactobacillus rhamnosus GG ameliorates triptolide-induced liver injury through modulation of the bile acid-FXR axis. Pharmacological research, 2024 (PubMed: 38908615) [IF=9.3]

5). Autophagic-CTSB-inflammasome axis modulates hepatic stellate cells activation in arsenic-induced liver fibrosis. Chemosphere, 2020 (PubMed: 31669990) [IF=8.8]

Application: WB Species: rat Sample: livers

Fig.2 |NaAsO2 induced autophagy, activation of NLRP3 inflammasome and hepatic stellate cell in vivo. (A) The expression level of LC3 and p62 in rat liver following exposure to NaAsO2. The protein fraction was analyzed by Western blot. (B)The expression level of cytoplasmic CTSB in rat livers following exposure to NaAsO2.The protein fraction was analyzed by Western blot. (C) The expression level of NLRP3 inflammasome-associated proteins in rat livers following exposure to NaAsO2.The protein fraction was analyzed by Western blot.

Application: WB Species: rat Sample: livers

Fig.2 NaAsO2 induced autophagy, activation of NLRP3 inflammasome and 662 hepatic stellate cell in vivo. (A) The expression level of LC3 and p62 in rat livers 663 following exposure to NaAsO2. The protein fraction was analyzed by Western blot. (B) 664 The expression level of cytoplasmic CTSB in rat livers following exposure to NaAsO2. 665 The protein fraction was analyzed by Western blot. (C) The expression level of NLRP3 inflammasome-associated proteins in rat livers following exposure to NaAsO2. 667 The protein fraction was analyzed by Western blot. (D) The expression level of 668 Collagen- , α-SMA, MMP-1, and TIMP-1in rat livers following exposure to NaAsO2. 669 The protein fraction was analyzed by Western blot. (E) α-SMA was detected by IF. 670 White arrows indicate positive staining. Values are mean ± SD, and n = 5. *p < 0.05, 671 **p < 0.01 compared with the control group; Scale bar = 100 µm

6). Combination of resolvin E1 and lipoxin A4 promotes the resolution of pulpitis by inhibiting NF-κB activation through upregulating sirtuin 7 in dental pulp fibroblasts. CELL PROLIFERATION, 2022 (PubMed: 35411569) [IF=8.5]

Application: WB Species: rat Sample: Dental pulp fibroblasts

FIGURE 1 |Effects of combined administration of RvE1 and LXA4 on pro-inflammatory factor expression.(C) NLRP3, (D) caspase-1, (E) IL-1β and (F) IL-18 mRNA levels on LPS-induced DPFs detected by qPCR and (G) their protein levels tested by western blotting (normalized to that of β-tubulin).

7). Gegen Qinlian pills alleviate carrageenan-induced thrombosis in mice model by regulating the HMGB1/NF-κB/NLRP3 signaling. PHYTOMEDICINE, 2022 (PubMed: 35413645) [IF=7.9]

8). Cinnamic acid preserves against myocardial ischemia/reperfusion injury via suppression of NLRP3/Caspase-1/GSDMD signaling pathway. Phytomedicine, 2022 (PubMed: 35320770) [IF=7.9]

9). Cardioprotective activity of ethyl acetate extract of Cinnamomi Ramulus against myocardial ischemia/reperfusion injury in rats via inhibiting NLRP3 inflammasome activation and pyroptosis. Phytomedicine, 2021 (PubMed: 34673348) [IF=7.9]

10). Iguratimod ameliorates bleomycin-induced pulmonary fibrosis by inhibiting the EMT process and NLRP3 inflammasome activation. Biomedicine & Pharmacotherapy, 2022 (PubMed: 36076570) [IF=7.5]

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

Caspase 1 Antibody - #AF5418

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Immunogens

Predictions

PTMs - P29466 As Substrate

Research Backgrounds

Research Fields

References

Restrictive clause