ATRIP Antibody | Affinity Biosciences

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Western blot analysis of ATRIP expression in Human mammary cell lysate.

Product:	ATRIP Antibody
Catalog:	AF5417
Description:	Rabbit polyclonal antibody to ATRIP
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Bovine, Sheep, Rabbit
Mol.Wt.:	85 kDa; 86kD(Calculated).
Uniprot:	Q8WXE1
RRID:	AB_2837901

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Bovine(100%), Sheep(83%), Rabbit(86%)

Clonality:

Polyclonal

Specificity:

ATRIP Antibody detects endogenous levels of total ATRIP.

RRID:

AB_2837901
Cite Format: Affinity Biosciences Cat# AF5417, RRID:AB_2837901.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AGS1; ATIP; ATM and Rad3 related interacting protein; ATM and Rad3-related-interacting protein; ATR interacting protein; ATR-interacting protein; Atrip; ATRIP_HUMAN; DKFZp762J2115; FLJ12343; MGC20625; MGC21482; MGC26740;

Immunogens

Immunogen:

Uniprot:

Q8WXE1(ATRIP_HUMAN) >>Visit HPA database.

Gene(ID):

ATRIP(84126)

Expression:

Q8WXE1 ATRIP_HUMAN:

Ubiquitous.

Description:

Required for checkpoint signaling after DNA damage. Required for ATR expression, possibly by stabilizing the protein.

Sequence:

Q8WXE1 ATRIP_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAGTSAPGSKRRSEPPAPRPGPPPGTGHPPSKRARGFSAAAAPDPDDPFGAHGDFTADDLEELDTLASQALSQCPAAARDVSSDHKVHRLLDGMSKNPSGKNRETVPIKDNFELEVLQAQYKELKEKMKVMEEEVLIKNGEIKILRDSLHQTESVLEEQRRSHFLLEQEKTQALSDKEKEFSKKLQSLQSELQFKDAEMNELRTKLQTSERANKLAAPSVSHVSPRKNPSVVIKPEACSPQFGKTSFPTKESFSANMSLPHPCQTESGYKPLVGREDSKPHSLRGDSIKQEEAQKSFVDSWRQRSNTQGSILINLLLKQPLIPGSSLSLCHLLSSSSESPAGTPLQPPGFGSTLAGMSGLRTTGSYDGSFSLSALREAQNLAFTGLNLVARNECSRDGDPAEGGRRAFPLCQLPGAVHFLPLVQFFIGLHCQALQDLAAAKRSGAPGDSPTHSSCVSSGVETNPEDSVCILEGFSVTALSILQHLVCHSGAVVSLLLSGVGADSAAGEGNRSLVHRLSDGDMTSALRGVADDQGQHPLLKMLLHLLAFSSAATGHLQASVLTQCLKVLVKLAENTSCDFLPRFQCVFQVLPKCLSPETPLPSVLLAVELLSLLADHDQLAPQLCSHSEGCLLLLLYMYITSRPDRVALETQWLQLEQEVVWLLAKLGVQSPLPPVTGSNCQCNVEVVRALTVMLHRQWLTVRRAGGPPRTDQQRRTVRCLRDTVLLLHGLSQKDKLFMMHCVEVLHQFDQVMPGVSMLIRGLPDVTDCEEAALDDLCAAETDVEDPEVECG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

100

Rabbit

Sheep

Pig

Horse

Dog

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8WXE1 As Substrate

Q8WXE1

Site	PTM Type	Enzyme	Source
S9	Phosphorylation		Uniprot
S13	Phosphorylation		Uniprot
T26	Phosphorylation		Uniprot
S31	Phosphorylation		Uniprot
K32	Acetylation		Uniprot
S38	Phosphorylation		Uniprot
S68	Phosphorylation	Q13535 (ATR)	Uniprot
S72	Phosphorylation	Q13535 (ATR)	Uniprot
K96	Ubiquitination		Uniprot
K122	Ubiquitination		Uniprot
S162	Phosphorylation		Uniprot
K205	Ubiquitination		Uniprot
K214	Ubiquitination		Uniprot
S219	Phosphorylation		Uniprot
S221	Phosphorylation		Uniprot
S224	Phosphorylation	P24941 (CDK2)	Uniprot
S239	Phosphorylation	P24941 (CDK2)	Uniprot
K270	Ubiquitination		Uniprot
K295	Ubiquitination		Uniprot
S305	Phosphorylation		Uniprot
T307	Phosphorylation		Uniprot
S310	Phosphorylation		Uniprot
S512	Phosphorylation		Uniprot
S518	Phosphorylation		Uniprot
T523	Phosphorylation		Uniprot
Y636	Phosphorylation		Uniprot

Research Backgrounds

Q8WXE1_ATRIP_HUMAN

Function:

Required for checkpoint signaling after DNA damage. Required for ATR expression, possibly by stabilizing the protein.

PTMs:

Phosphorylated by ATR.

Subcellular Location:

Nucleus.
Note: Redistributes to discrete nuclear foci upon DNA damage.

Tissue Specificity:

Ubiquitous.

Subunit Structure:

Interacts with ATR (By similarity). Heterodimer with ATR. The heterodimer binds the RPA complex and is then recruited to single-stranded DNA. Interacts with CEP164 (via N-terminus). Interacts with CINP.

Family&Domains:

The EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage.

Belongs to the ATRIP family.

Research Fields

· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.

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ATRIP Antibody - #AF5417