Stathmin 1 Antibody - #AF5414

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Product: Stathmin 1 Antibody
Catalog: AF5414
Description: Rabbit polyclonal antibody to Stathmin 1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 17 kDa; 17kD(Calculated).
Uniprot: P16949
RRID: AB_2837898

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(88%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Stathmin 1 Antibody detects endogenous levels of total Stathmin 1.
RRID:
AB_2837898
Cite Format: Affinity Biosciences Cat# AF5414, RRID:AB_2837898.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

C1orf215; Lag; LAP 18; LAP18; Leukemia associated phosphoprotein p18; Leukemia-associated phosphoprotein p18; Metablastin; Oncoprotein 18; OP 18; Op18; p18; p19; Phosphoprotein 19; Phosphoprotein p19; pp17; pp19; PR22; Pr22 protein; Prosolin; Protein Pr22; SMN; Stathmin; Stathmin1; STMN 1; Stmn1; STMN1_HUMAN;

Immunogens

Immunogen:
Uniprot:

P16949(STMN1_HUMAN) >>Visit HPA database.

Gene(ID):
STMN1(3925)
Expression:
P16949 STMN1_HUMAN:

Ubiquitous. Expression is strongest in fetal and adult brain, spinal cord, and cerebellum, followed by thymus, bone marrow, testis, and fetal liver. Expression is intermediate in colon, ovary, placenta, uterus, and trachea, and is readily detected at substantially lower levels in all other tissues examined. Lowest expression is found in adult liver. Present in much greater abundance in cells from patients with acute leukemia of different subtypes than in normal peripheral blood lymphocytes, non-leukemic proliferating lymphoid cells, bone marrow cells, or cells from patients with chronic lymphoid or myeloid leukemia.

Description:
This protein is a ubiquitous cytosolic phosphoprotein proposed to function as an intracellular relay integrating regulatory signals of the cellular environment. The encoded protein is involved in the regulation of the microtubule filament system by destabilizing microtubules. It prevents assembly and promotes disassembly of microtubules.
Sequence:
MASSDIQVKELEKRASGQAFELILSPRSKESVPEFPLSPPKKKDLSLEEIQKKLEAAEERRKSHEAEVLKQLAEKREHEKEVLQKAIEENNNFSKMAEEKLTHKMEANKENREAQMAAKLERLREKDKHIEEVRKNKESKDPADETEAD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Chicken
88
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P16949 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S3 Phosphorylation
S4 Phosphorylation
K9 Acetylation
K9 Ubiquitination
R14 Methylation
S16 Phosphorylation Q13153 (PAK1) , Q16566 (CAMK4) , Q13554 (CAMK2B) , P17612 (PRKACA) , Q9UQM7 (CAMK2A)
S25 Phosphorylation P53778 (MAPK12) , P45983 (MAPK8) , P24941 (CDK2) , P27361 (MAPK3) , Q15759 (MAPK11) , P05771 (PRKCB) , P45984 (MAPK9) , O15264 (MAPK13) , P06493 (CDK1) , Q16539 (MAPK14) , P53779 (MAPK10) , P28482 (MAPK1)
R27 Methylation
S28 Phosphorylation
K29 Acetylation
K29 Methylation
K29 Ubiquitination
S31 Phosphorylation
S38 Phosphorylation Q00535 (CDK5) , O15264 (MAPK13) , P45983 (MAPK8) , Q8TAS1 (UHMK1) , Q13153 (PAK1) , P05771 (PRKCB) , P06493 (CDK1) , P28482 (MAPK1) , P53779 (MAPK10) , P24941 (CDK2) , P45984 (MAPK9) , P27361 (MAPK3)
K41 Ubiquitination
K43 Ubiquitination
S46 Phosphorylation
K52 Acetylation
K52 Ubiquitination
K53 Acetylation
K53 Ubiquitination
K62 Ubiquitination
S63 Phosphorylation P17612 (PRKACA)
K70 Acetylation
K70 Ubiquitination
K75 Ubiquitination
K80 Acetylation
K80 Ubiquitination
K85 Ubiquitination
S94 Phosphorylation
K95 Acetylation
K95 Ubiquitination
K100 Acetylation
K100 Ubiquitination
T102 Phosphorylation
K104 Acetylation
K104 Ubiquitination
K109 Acetylation
K119 Acetylation
K119 Ubiquitination
K128 Acetylation
K128 Ubiquitination
R134 Methylation
K137 Acetylation
K137 Ubiquitination
K140 Ubiquitination
T146 Phosphorylation

Research Backgrounds

Function:

Involved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear (By similarity).

PTMs:

Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. MAPK is responsible for the phosphorylation of stathmin in response to NGF. Phosphorylation at Ser-16 seems to be required for neuron polarization (By similarity). Phosphorylation at Ser-63 reduces tubulin binding 10-fold and suppresses the MT polymerization inhibition activity.

Subcellular Location:

Cytoplasm>Cytoskeleton.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous. Expression is strongest in fetal and adult brain, spinal cord, and cerebellum, followed by thymus, bone marrow, testis, and fetal liver. Expression is intermediate in colon, ovary, placenta, uterus, and trachea, and is readily detected at substantially lower levels in all other tissues examined. Lowest expression is found in adult liver. Present in much greater abundance in cells from patients with acute leukemia of different subtypes than in normal peripheral blood lymphocytes, non-leukemic proliferating lymphoid cells, bone marrow cells, or cells from patients with chronic lymphoid or myeloid leukemia.

Subunit Structure:

Binds to two alpha/beta-tubulin heterodimers. Interacts with KIST.

Family&Domains:

Belongs to the stathmin family.

Research Fields

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

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