TXNRD2 Antibody | Affinity Biosciences

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Western blot analysis of TRXR2 expression in HT29 cells.

Product:	TXNRD2 Antibody
Catalog:	AF5399
Description:	Rabbit polyclonal antibody to TXNRD2
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.:	56 kDa; 57kD(Calculated).
Uniprot:	Q9NNW7
RRID:	AB_2837884

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(93%), Bovine(93%), Horse(87%), Sheep(93%), Rabbit(93%), Dog(87%), Xenopus(80%)

Clonality:

Polyclonal

Specificity:

TXNRD2 Antibody detects endogenous levels of total TXNRD2.

RRID:

AB_2837884
Cite Format: Affinity Biosciences Cat# AF5399, RRID:AB_2837884.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

mitochondrial; Selenoprotein Z; SelZ; Thioredoxin reductase 2; Thioredoxin reductase 2 mitochondrial; Thioredoxin reductase 3; Thioredoxin reductase beta; Thioredoxin reductase TR3; TR 3; TR; TR beta; TR-beta; TR3; TRXR 2; TRXR2; TRXR2_HUMAN; TXNRD 2; Txnrd2;

Immunogens

Immunogen:

A synthesized peptide derived from human TXNRD2, corresponding to a region within C-terminal amino acids.

Uniprot:

Q9NNW7(TRXR2_HUMAN) >>Visit HPA database.

Gene(ID):

TXNRD2(10587)

Expression:

Q9NNW7 TRXR2_HUMAN:

Highly expressed in the prostate, ovary, liver, testis, uterus, colon and small intestine. Intermediate levels in brain, skeletal muscle, heart and spleen. Low levels in placenta, pancreas, thymus and peripheral blood leukocytes. According to PubMed:10608886, high levels in kidney, whereas according to PubMed:9923614, levels are low. High expression is observed in the adrenal cortex (PubMed:24601690).

Description:

Maintains thioredoxin in a reduced state. Implicated in the defenses against oxidative stress. May play a role in redox-regulated cell signaling.

Sequence:

Q9NNW7 TRXR2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAMAVALRGLGGRFRWRTQAVAGGVRGAARGAAAGQRDYDLLVVGGGSGGLACAKEAAQLGRKVAVVDYVEPSPQGTRWGLGGTCVNVGCIPKKLMHQAALLGGLIQDAPNYGWEVAQPVPHDWRKMAEAVQNHVKSLNWGHRVQLQDRKVKYFNIKASFVDEHTVCGVAKGGKEILLSADHIIIATGGRPRYPTHIEGALEYGITSDDIFWLKESPGKTLVVGASYVALECAGFLTGIGLDTTIMMRSIPLRGFDQQMSSMVIEHMASHGTRFLRGCAPSRVRRLPDGQLQVTWEDSTTGKEDTGTFDTVLWAIGRVPDTRSLNLEKAGVDTSPDTQKILVDSREATSVPHIYAIGDVVEGRPELTPIAIMAGRLLVQRLFGGSSDLMDYDNVPTTVFTPLEYGCVGLSEEEAVARHGQEHVEVYHAHYKPLEFTVAGRDASQCYVKMVCLREPPQLVLGLHFLGPNAGEVTQGFALGIKCGASYAQVMRTVGIHPTCSEEVVKLRISKRSGLDPTVTGCUG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Bovine

Sheep

Rabbit

Horse

Dog

Xenopus

Chicken

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NNW7 As Substrate

Q9NNW7

Site	PTM Type	Source
Y40	Phosphorylation	Uniprot
K64	Ubiquitination	Uniprot
Y70	Phosphorylation	Uniprot
K94	Ubiquitination	Uniprot
K137	Acetylation	Uniprot
K137	Ubiquitination	Uniprot
K153	Acetylation	Uniprot
K175	Acetylation	Uniprot
S180	Phosphorylation	Uniprot
T238	Phosphorylation	Uniprot
T244	Phosphorylation	Uniprot
T245	Phosphorylation	Uniprot
S250	Phosphorylation	Uniprot
K329	Ubiquitination	Uniprot
K340	Ubiquitination	Uniprot
K432	Acetylation	Uniprot
K506	Acetylation	Uniprot

Research Backgrounds

Q9NNW7_TRXR2_HUMAN

Function:

Involved in the control of reactive oxygen species levels and the regulation of mitochondrial redox homeostasis. Maintains thioredoxin in a reduced state. May play a role in redox-regulated cell signaling.

Subcellular Location:

Mitochondrion.

Tissue Specificity:

Highly expressed in the prostate, ovary, liver, testis, uterus, colon and small intestine. Intermediate levels in brain, skeletal muscle, heart and spleen. Low levels in placenta, pancreas, thymus and peripheral blood leukocytes. According to high levels in kidney, whereas according to levels are low. High expression is observed in the adrenal cortex.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Research Fields

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma. (View pathway)

· Metabolism > Metabolism of other amino acids > Selenocompound metabolism.

Restrictive clause

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For Research Use Only.
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TXNRD2 Antibody - #AF5399