VDAC2 Antibody - #AF5397

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Product: VDAC2 Antibody
Catalog: AF5397
Description: Rabbit polyclonal antibody to VDAC2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 32 kDa; 32kD(Calculated).
Uniprot: P45880
RRID: AB_2837882

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 100ul $280 In stock
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Lead Time: Same day delivery

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(89%), Bovine(89%), Horse(89%), Sheep(89%), Rabbit(89%), Dog(89%)
Clonality:
Polyclonal
Specificity:
VDAC2 Antibody detects endogenous levels of total VDAC2.
RRID:
AB_2837882
Cite Format: Affinity Biosciences Cat# AF5397, RRID:AB_2837882.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

B-36 vdac; FLJ23841; hVDAC2; OTTHUMP00000019878; OTTHUMP00000019879; OTTHUMP00000019880; Outer mitochondrial membrane protein porin 2; POR; Porin-2; VDAC 2; VDAC-2; VDAC2; VDAC2_HUMAN; Vdac6; Voltage dependent anion channel 2; Voltage dependent anion selective channel protein 2; Voltage-dependent anion-selective channel protein 2;

Immunogens

Immunogen:
Uniprot:

P45880(VDAC2_HUMAN) >>Visit HPA database.

Gene(ID):
VDAC2(7417)
Expression:
P45880 VDAC2_HUMAN:

Expressed in all tissues examined.

Description:
Forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective.
Sequence:
MATHGQTCARPMCIPPSYADLGKAARDIFNKGFGFGLVKLDVKTKSCSGVEFSTSGSSNTDTGKVTGTLETKYKWCEYGLTFTEKWNTDNTLGTEIAIEDQICQGLKLTFDTTFSPNTGKKSGKIKSSYKRECINLGCDVDFDFAGPAIHGSAVFGYEGWLAGYQMTFDSAKSKLTRNNFAVGYRTGDFQLHTNVNDGTEFGGSIYQKVCEDLDTSVNLAWTSGTNCTRFGIAAKYQLDPTASISAKVNNSSLIGVGYTQTLRPGVKLTLSALVDGKSINAGGHKVGLALELEA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
89
Horse
89
Bovine
89
Sheep
89
Dog
89
Rabbit
89
Xenopus
78
Zebrafish
78
Chicken
78
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P45880 As Substrate

Site PTM Type Enzyme
Ubiquitination
A2 Acetylation
T7 Phosphorylation
K23 Ubiquitination
K31 Acetylation
K31 Ubiquitination
K39 Acetylation
K39 Ubiquitination
T44 Phosphorylation
K45 Ubiquitination
S46 Phosphorylation
C47 S-Nitrosylation
S48 Phosphorylation
T54 Phosphorylation
S55 Phosphorylation
S57 Phosphorylation
S58 Phosphorylation
K64 Sumoylation
K64 Ubiquitination
T66 Phosphorylation
T68 Phosphorylation
K72 Acetylation
K72 Ubiquitination
Y73 Phosphorylation
K74 Acetylation
K74 Ubiquitination
C76 S-Nitrosylation
Y78 Phosphorylation
T109 Phosphorylation
T112 Phosphorylation
T113 Phosphorylation
S115 Phosphorylation
T118 Phosphorylation
K120 Acetylation
K120 Ubiquitination
K121 Ubiquitination
K124 Ubiquitination
S128 Phosphorylation
K172 Sumoylation
Y184 Phosphorylation
Y206 Phosphorylation
K235 Acetylation
K235 Ubiquitination
S245 Phosphorylation
K247 Ubiquitination
S251 Phosphorylation
S252 Phosphorylation
Y258 Phosphorylation
T259 Phosphorylation
T261 Phosphorylation
K277 Acetylation
K277 Ubiquitination
K285 Ubiquitination

Research Backgrounds

Function:

Forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. The channel adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective.

PTMs:

Ubiquitinated by PRKN during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30.

Subcellular Location:

Mitochondrion outer membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in all tissues examined.

Subunit Structure:

Interacts with hexokinases.

Family&Domains:

Consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands.

Belongs to the eukaryotic mitochondrial porin family.

Research Fields

· Cellular Processes > Cell growth and death > Ferroptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Digestive system > Cholesterol metabolism.

References

1). Deubiquitinase UCHL1 regulates estradiol synthesis by stabilizing voltage-dependent anion channel 2. Journal of Biological Chemistry, 2023 (PubMed: 37797697) [IF=4.8]

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