Cleaved-Caspase 6 (Asp179) Antibody

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Western blot analysis of Caspase 6 (Cleaved-Asp179) expression in Hela cells.

AF5381 at 1/100 staining Rat heart tissue by IHC-P.

Product:	Cleaved-Caspase 6 (Asp179) Antibody
Catalog:	AF5381
Description:	Rabbit polyclonal antibody to Cleaved-Caspase 6 (Asp179)
Application:	WB IHC IF/ICC
Reactivity:	Human, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Dog
Mol.Wt.:	18 kDa; 33kD(Calculated).
Uniprot:	P55212
RRID:	AB_2837866

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Rat

Prediction:

Pig(100%), Zebrafish(83%), Bovine(100%), Horse(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

Cleaved-Caspase 6 (Asp179) Antibody detects endogenous levels of fragment of activated Caspase 6 resulting from cleavage adjacent to Asp179.

RRID:

AB_2837866
Cite Format: Affinity Biosciences Cat# AF5381, RRID:AB_2837866.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Apoptotic protease Mch-2; Apoptotic protease MCH2; CASP-6; CASP6; CASP6_HUMAN; Caspase 6; Caspase 6 apoptosis related cysteine protease; Caspase 6, apoptosis related cysteine peptidase; Caspase-6 subunit p11; Mch2; OTTHUMP00000162742; OTTHUMP00000162743;

Immunogens

Immunogen:

Uniprot:

P55212(CASP6_HUMAN) >>Visit HPA database.

Gene(ID):

CASP6(839)

Description:

Caspase-6 (Mch2) is one of the major executioner caspases functioning in cellular apoptotic processes. Upon apoptotic stimulation, initiator caspases such as caspase-9 are cleaved and activated.

Sequence:

P55212 CASP6_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSSASGLRRGHPAGGEENMTETDAFYKREMFDPAEKYKMDHRRRGIALIFNHERFFWHLTLPERRGTCADRDNLTRRFSDLGFEVKCFNDLKAEELLLKIHEVSTVSHADADCFVCVFLSHGEGNHIYAYDAKIEIQTLTGLFKGDKCHSLVGKPKIFIIQACRGNQHDVPVIPLDVVDNQTEKLDTNITEVDAASVYTLPAGADFLMCYSVAEGYYSHRETVNGSWYIQDLCEMLGKYGSSLEFTELLTLVNRKVSQRRVDFCKDPSAIGKKQVPCFASMLTKKLHFFPKSN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Dog

100

Zebrafish

Sheep

Xenopus

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P55212 As Substrate

P55212

Site	PTM Type	Enzyme	Source
R9	Methylation		Uniprot
K27	Ubiquitination		Uniprot
K36	Acetylation		Uniprot
K36	Ubiquitination		Uniprot
S79	Phosphorylation		Uniprot
K92	Ubiquitination		Uniprot
K144	Ubiquitination		Uniprot
K147	Ubiquitination		Uniprot
K154	Ubiquitination		Uniprot
S257	Phosphorylation	O60285 (NUAK1)	Uniprot
K265	Ubiquitination		Uniprot
K272	Ubiquitination		Uniprot
K273	Acetylation		Uniprot
K273	Ubiquitination		Uniprot
K284	Acetylation		Uniprot
K285	Acetylation		Uniprot
K285	Ubiquitination		Uniprot

Research Backgrounds

P55212_CASP6_HUMAN

Function:

Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death.

PTMs:

Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits.

Subcellular Location:

Cytoplasm.

Subunit Structure:

Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 11 kDa (p11) subunit. Interacts with BIRC6/bruce.

Family&Domains:

Belongs to the peptidase C14A family.

Research Fields

· Cellular Processes > Cell growth and death > Apoptosis. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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Cleaved-Caspase 6 (Asp179) Antibody - #AF5381