PAR4 Antibody - #AF5371

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Product: PAR4 Antibody
Catalog: AF5371
Description: Rabbit polyclonal antibody to PAR4
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Rabbit, Chicken, Xenopus
Mol.Wt.: 37 kDa; 37kD(Calculated).
Uniprot: Q96IZ0
RRID: AB_2837856

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Rabbit(88%), Chicken(100%), Xenopus(90%)
Clonality:
Polyclonal
Specificity:
PAR4 Antibody detects endogenous levels of total PAR4.
RRID:
AB_2837856
Cite Format: Affinity Biosciences Cat# AF5371, RRID:AB_2837856.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

2310001G03Rik; PAR 4; PAR-4; Pawr; PAWR_HUMAN; PRKC Apoptosis WT1 Regulator; PRKC apoptosis WT1 regulator protein; Prostate apoptosis response 4 protein; Prostate apoptosis response protein 4; prostate apoptosis response protein PAR-4; Transcriptional repressor Par-4-like protein PAWR; Transcriptional repressor PAR4; WT1 Interacting Protein;

Immunogens

Immunogen:
Uniprot:

Q96IZ0(PAWR_HUMAN) >>Visit HPA database.

Gene(ID):
PAWR(5074)
Expression:
Q96IZ0 PAWR_HUMAN:

Widely expressed. Expression is elevated in various neurodegenerative diseases such as amyotrophic lateral sclerosis, Alzheimer, Parkinson and Huntington diseases and stroke. Down-regulated in several cancers.

Description:
Pro-apoptopic protein capable of selectively inducing apoptosis in cancer cells, sensitizing the cells to diverse apoptotic stimuli and causing regression of tumors in animal models. Induces apoptosis in certain cancer cells by activation of the Fas prodeath pathway and coparallel inhibition of NF-kappa-B transcriptional activity.
Sequence:
MATGGYRTSSGLGGSTTDFLEEWKAKREKMRAKQNPPGPAPPGGGSSDAAGKPPAGALGTPAAAAANELNNNLPGGAPAAPAVPGPGGVNCAVGSAMLTRAAPGPRRSEDEPPAASASAAPPPQRDEEEPDGVPEKGKSSGPSARKGKGQIEKRKLREKRRSTGVVNIPAAECLDEYEDDEAGQKERKREDAITQQNTIQNEAVNLLDPGSSYLLQEPPRTVSGRYKSTTSVSEEDVSSRYSRTDRSGFPRYNRDANVSGTLVSSSTLEKKIEDLEKEVVRERQENLRLVRLMQDKEEMIGKLKEEIDLLNRDLDDIEDENEQLKQENKTLLKVVGQLTR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Zebrafish
100
Pig
100
Chicken
100
Xenopus
90
Rabbit
88
Horse
0
Bovine
0
Sheep
0
Dog
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96IZ0 As Substrate

Site PTM Type Enzyme
T8 Phosphorylation
S15 Phosphorylation
S95 Phosphorylation
T99 Phosphorylation
S108 Phosphorylation
K146 Methylation
S162 Phosphorylation
T163 Phosphorylation
C173 S-Nitrosylation
Y177 Phosphorylation
S223 Phosphorylation
Y226 Phosphorylation
K227 Methylation
K227 Ubiquitination
S228 Phosphorylation
T229 Phosphorylation
T230 Phosphorylation
S231 Phosphorylation P68400 (CSNK2A1)
S233 Phosphorylation
Y241 Phosphorylation
S242 Phosphorylation
S259 Phosphorylation
T261 Phosphorylation
T267 Phosphorylation
K277 Ubiquitination
K296 Acetylation
K296 Ubiquitination
K302 Acetylation
K304 Ubiquitination
K329 Ubiquitination
K333 Ubiquitination

Research Backgrounds

Function:

Pro-apoptotic protein capable of selectively inducing apoptosis in cancer cells, sensitizing the cells to diverse apoptotic stimuli and causing regression of tumors in animal models. Induces apoptosis in certain cancer cells by activation of the Fas prodeath pathway and coparallel inhibition of NF-kappa-B transcriptional activity. Inhibits the transcriptional activation and augments the transcriptional repression mediated by WT1. Down-regulates the anti-apoptotic protein BCL2 via its interaction with WT1. Seems also to be a transcriptional repressor by itself. May be directly involved in regulating the amyloid precursor protein (APP) cleavage activity of BACE1.

PTMs:

Preferentially phosphorylated at the Thr-163 by PKC in cancer cells.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Mainly cytoplasmic in absence of apoptosis signal and in normal cells. Nuclear in most cancer cell lines. Nuclear entry seems to be essential but not sufficient for apoptosis (By similarity). Nuclear localization includes nucleoplasm and PML nuclear bodies.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed. Expression is elevated in various neurodegenerative diseases such as amyotrophic lateral sclerosis, Alzheimer, Parkinson and Huntington diseases and stroke. Down-regulated in several cancers.

Subunit Structure:

Homooligomer. Interacts (via the C-terminal region) with WT1. Interacts with THAP1. Interacts with AATF. Interacts with BACE1. Interacts with SPSB1 (via B30.2/SPRY domain); this interaction is direct and occurs in association with the Elongin BC complex. Interacts with SPSB2 (via B30.2/SPRY domain); this interaction occurs in association with the Elongin BC complex. Interacts with SPSB4 (via B30.2/SPRY domain); this interaction occurs in association with the Elongin BC complex. Component of a ternary complex composed of SQSTM1 and PRKCZ. Interacts with actin (By similarity).

Family&Domains:

The leucine-zipper domain is not essential for apoptosis, but is required for sensitization of cells to exogenous apoptotic insults and for interaction with its partners.

The SAC domain is a death-inducing domain selective for apoptosis induction in cancer cells. This domain is essential for nuclear entry, Fas activation, inhibition of NF-kappa-B activity and induction of apoptosis in cancer cells (By similarity).

The B30.2/SPRY domain-binding motif mediates recognition by proteins containing a B30.2/SPRY domain.

References

1). MicroRNA-26a alleviates tubulointerstitial fibrosis in diabetic kidney disease by targeting PAR4. Journal of cellular and molecular medicine, 2024 (PubMed: 38164021) [IF=5.3]
2). Mannose binding lectin-associated serine protease-1 is a novel contributor to myocardial ischemia/reperfusion injury. International Journal of Cardiology, 2023 (PubMed: 37473815) [IF=3.5]

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