Cytokeratin 14 Antibody - #AF5370

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Product: Cytokeratin 14 Antibody
Catalog: AF5370
Description: Rabbit polyclonal antibody to Cytokeratin 14
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Rabbit, Dog
Mol.Wt.: 52 kDa; 52kD(Calculated).
Uniprot: P02533
RRID: AB_2837855

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Rabbit(90%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Cytokeratin 14 Antibody detects endogenous levels of total Cytokeratin 14.
RRID:
AB_2837855
Cite Format: Affinity Biosciences Cat# AF5370, RRID:AB_2837855.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CK 14; CK-14; ck14; Cytokeratin 14; Cytokeratin-14; Cytokeratin14; Dowling Meara; EBS3; EBS4; Epidermolysis bullosa simplex; K14; K1C14_HUMAN; Keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner); Keratin 14; Keratin; Keratin type I cytoskeletal 14; Keratin, type I cytoskeletal 14; Keratin-14; Keratin14; Koebner; Krt 14; Krt14; NFJ; OTTHUMP00000164624; type I cytoskeletal 14;

Immunogens

Immunogen:
Uniprot:

P02533(K1C14_HUMAN) >>Visit HPA database.

Gene(ID):
KRT14(3861)
Expression:
P02533 K1C14_HUMAN:

Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen.

Description:
The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.
Sequence:
MTTCSRQFTSSSSMKGSCGIGGGIGGGSSRISSVLAGGSCRAPSTYGGGLSVSSSRFSSGGACGLGGGYGGGFSSSSSSFGSGFGGGYGGGLGAGLGGGFGGGFAGGDGLLVGSEKVTMQNLNDRLASYLDKVRALEEANADLEVKIRDWYQRQRPAEIKDYSPYFKTIEDLRNKILTATVDNANVLLQIDNARLAADDFRTKYETELNLRMSVEADINGLRRVLDELTLARADLEMQIESLKEELAYLKKNHEEEMNALRGQVGGDVNVEMDAAPGVDLSRILNEMRDQYEKMAEKNRKDAEEWFFTKTEELNREVATNSELVQSGKSEISELRRTMQNLEIELQSQLSMKASLENSLEETKGRYCMQLAQIQEMIGSVEEQLAQLRCEMEQQNQEYKILLDVKTRLEQEIATYRRLLEGEDAHLSSSQFSSGSQSSRDVTSSSRQIRTKVMDVHDGKVVSTHEQVLRTKN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
100
Rabbit
90
Chicken
70
Pig
0
Horse
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P02533 As Substrate

Site PTM Type Enzyme
T9 Phosphorylation
S10 Phosphorylation
S12 Phosphorylation
S13 Phosphorylation
K15 Methylation
S17 Phosphorylation
S28 Phosphorylation
S32 Phosphorylation
S33 Phosphorylation
S39 Phosphorylation
S44 Phosphorylation
T45 Phosphorylation
Y46 Phosphorylation
S53 Phosphorylation
S128 Phosphorylation
Y129 Phosphorylation
K132 Acetylation
K132 Ubiquitination
Y151 Phosphorylation
S163 Phosphorylation
Y165 Phosphorylation
Y204 Phosphorylation
S213 Phosphorylation
K243 Methylation
Y248 Phosphorylation
K250 Sumoylation
K250 Ubiquitination
K251 Ubiquitination
Y291 Phosphorylation
K293 Ubiquitination
T319 Phosphorylation
S321 Phosphorylation
S326 Phosphorylation
K328 Ubiquitination
S332 Phosphorylation
S358 Phosphorylation
K363 Ubiquitination
Y398 Phosphorylation
K399 Ubiquitination
K405 Ubiquitination
T414 Phosphorylation
Y415 Phosphorylation
S429 Phosphorylation
S432 Phosphorylation
S433 Phosphorylation
S435 Phosphorylation
S437 Phosphorylation
S438 Phosphorylation

Research Backgrounds

Function:

The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.

PTMs:

A disulfide bond is formed between rather than within filaments and promotes the formation of a keratin filament cage around the nucleus.

Ubiquitinated by the BCR(KLHL24) E3 ubiquitin ligase complex.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Expressed in both as a filamentous pattern.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen.

Subunit Structure:

Heterotetramer of two type I and two type II keratins. disulfide-linked keratin-14 associates with keratin-5. Interacts with TRADD and with keratin filaments. Associates with other type I keratins. Interacts with EPPK1 (By similarity). Interacts with KLHL24.

Family&Domains:

Belongs to the intermediate filament family.

Research Fields

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

References

1). Small extracellular vesicles derived from hypoxic preconditioned dental pulp stem cells ameliorate inflammatory osteolysis by modulating macrophage polarization and osteoclastogenesis. Bioactive Materials, 2023 (PubMed: 36311048) [IF=18.9]
2). Dental follicle stem cells rescue the regenerative capacity of inflamed rat dental pulp through a paracrine pathway. Stem Cell Research & Therapy, 2020 (PubMed: 32746910) [IF=7.5]

Application: IF/ICC    Species: mouse    Sample: rDFSCs

Fig. 1 Isolation and identification of rDFSCs and rDPCs. Representative images of rDFSCs (a) and rDPCs (f). rDFSCs and rDPCs were both positive for vimentin (b, g) and negative for cytokeratin (c, h). After osteogenic induction for 14 days, mineralized nodules were detected in rDFSCs (d) and rDPCs (i) by Alizarin Red staining. After adipogenic induction for 14 days, lipid droplets were detected in rDFSCs (e) and rDPCs (j) by Oil Red O staining. Scale bar 100 μm. Representative flow cytometric results showed the expression pattern of CD markers in P3 rDFSCs (k–q) and rDPCs (r–x)
3). Hypoxia‑induced mitophagy regulates proliferation, migration and odontoblastic differentiation of human dental pulp cells through FUN14 domain‑containing 1. International Journal of Molecular Medicine, 2022 (PubMed: 35362539) [IF=5.4]

Application: IF/ICC    Species: Human    Sample: HDPCs

Figure 2 Identification of HDPCs. (A) Representative images of HDPCs at passage 3. HDPCs are (B) positive for vimentin and (C) negative for cytokeratin. (D) Representative image of mineralized nodules in HDPCs after osteogenic induction for 28 days following Alizarin Red staining. (E) Representative image of chondroblasts in HDPCs after chondrogenic induction for 21 days following Alcian Blue staining. (F) Representative image of lipid droplets in HDPCs after adipogenic induction for 28 days following Oil Red O staining. Scale bars, 50 µm. (G) Representative flow cytometry histograms showing the expression profile of markers in the p3 HDPCs. HDPCs, human dental pulp cells; VIM, vimentin; CK, cytokeratin.

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