p18 INK4c Antibody - #AF5356

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Product: p18 INK4c Antibody
Catalog: AF5356
Description: Rabbit polyclonal antibody to p18 INK4c
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 18 kDa; 18kD(Calculated).
Uniprot: P42773
RRID: AB_2837841

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Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(91%), Bovine(91%), Horse(82%), Sheep(91%), Rabbit(82%), Dog(91%)
Clonality:
Polyclonal
Specificity:
p18 INK4c Antibody detects endogenous levels of total p18 INK4c.
RRID:
AB_2837841
Cite Format: Affinity Biosciences Cat# AF5356, RRID:AB_2837841.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CDK6 inhibitor p18; CDKN 2C; CDKN 6; Cdkn2c; CDKN6; CDN2C_HUMAN; Cyclin dependent inhibitor; Cyclin dependent kinase 4 inhibitor C; Cyclin dependent kinase 6 inhibitor; Cyclin dependent kinase 6 inhibitor p18; Cyclin dependent kinase inhibitor 2C (p18 inhibits CDK4); Cyclin dependent kinase inhibitor 2C; Cyclin-dependent kinase 4 inhibitor C; Cyclin-dependent kinase 6 inhibitor; INK4C; OTTHUMP00000046546; p18; p18 inhibits CDK4; p18 INK4c; p18 INK6; p18-INK4c; p18-INK6;

Immunogens

Immunogen:
Uniprot:

P42773(CDN2C_HUMAN) >>Visit HPA database.

Gene(ID):
CDKN2C(1031)
Expression:
P42773 CDN2C_HUMAN:

Highest levels found in skeletal muscle. Also found in pancreas and heart.

Description:
Interacts strongly with CDK6, weakly with CDK4. Inhibits cell growth and proliferation with a correlated dependence on endogenous retinoblastoma protein RB.
Sequence:
MAEPWGNELASAAARGDLEQLTSLLQNNVNVNAQNGFGRTALQVMKLGNPEIARRLLLRGANPDLKDRTGFAVIHDAARAGFLDTLQTLLEFQADVNIEDNEGNLPLHLAAKEGHLRVVEFLVKHTASNVGHRNHKGDTACDLARLYGRNEVVSLMQANGAGGATNLQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
91
Bovine
91
Sheep
91
Dog
91
Horse
82
Rabbit
82
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P42773 As Substrate

Site PTM Type Enzyme
K46 Ubiquitination
K66 Ubiquitination
K136 Ubiquitination

Research Backgrounds

Function:

Interacts strongly with CDK6, weakly with CDK4. Inhibits cell growth and proliferation with a correlated dependence on endogenous retinoblastoma protein RB.

Tissue Specificity:

Highest levels found in skeletal muscle. Also found in pancreas and heart.

Subunit Structure:

Heterodimer of p18 with CDK6.

Family&Domains:

Belongs to the CDKN2 cyclin-dependent kinase inhibitor family.

Research Fields

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

References

1). Oxidative modification of miR-30c promotes cardiac fibroblast proliferation via CDKN2C mismatch. Scientific reports, 2024 (PubMed: 38849466) [IF=4.6]

Application: WB    Species: Mouse    Sample:

Figure 5. CDKN2C is the target of 4,5-oxo-miR-30c. (A) 4,5-oxo-miR-30c and homologies of its predicted target sites in the 3′UTR of CDKN2C mRNA. (B) Luciferase reporter assays for the 3′UTR of CDKN2C mRNA using synthesized 4,5-oxo-miR-30c, 4,5-mut-miR-30c, and miR-30c. Activity is reported relative to the nontarget control. *P 

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