Claudin 4 Antibody - #AF5350

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Product: Claudin 4 Antibody
Catalog: AF5350
Description: Rabbit polyclonal antibody to Claudin 4
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 22 kDa; 22kD(Calculated).
Uniprot: O14493
RRID: AB_2837835

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(93%), Bovine(93%), Horse(87%), Sheep(93%), Rabbit(93%), Dog(100%), Xenopus(80%)
Clonality:
Polyclonal
Specificity:
Claudin 4 Antibody detects endogenous levels of total Claudin 4.
RRID:
AB_2837835
Cite Format: Affinity Biosciences Cat# AF5350, RRID:AB_2837835.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Claudin-4; Claudin4; CLD4_HUMAN; CLDN 4; CLDN4; Clostridium perfringens enterotoxin receptor 1; Clostridium perfringens enterotoxin receptor; CPE R; CPE receptor; CPE-R; CPE-receptor; CPER; CPETR 1; CPETR; CPETR1; hCPE R; WBSCR8; Williams Beuren syndrome chromosome region 8 protein; Williams-Beuren syndrome chromosomal region 8 protein;

Immunogens

Immunogen:
Uniprot:

O14493(CLD4_HUMAN) >>Visit HPA database.

Gene(ID):
CLDN4(1364)
Description:
Note=CLDN4 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
Sequence:
MASMGLQVMGIALAVLGWLAVMLCCALPMWRVTAFIGSNIVTSQTIWEGLWMNCVVQSTGQMQCKVYDSLLALPQDLQAARALVIISIIVAALGVLLSVVGGKCTNCLEDESAKAKTMIVAGVVFLLAGLMVIVPVSWTAHNIIQDFYNPLVASGQKREMGASLYVGWAASGLLLLGGGLLCCNCPPRTDKPYSAKYSAARSAAASNYV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
100
Pig
93
Bovine
93
Sheep
93
Rabbit
93
Horse
87
Xenopus
80
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O14493 As Substrate

Site PTM Type Enzyme
T189 Phosphorylation
Y193 Phosphorylation
S194 Phosphorylation P17252 (PRKCA)
Y197 Phosphorylation
S198 Phosphorylation
S202 Phosphorylation
S206 Phosphorylation
Y208 Phosphorylation P29317 (EPHA2)

Research Backgrounds

Function:

Channel-forming tight junction protein that mediates paracellular chloride transport in the kidney. Plays a critical role in the paracellular reabsorption of filtered chloride in the kidney collecting ducts. Claudins play a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.

PTMs:

Phosphorylated. Phosphorylation by EPHA2 is stimulated by EFNA1 and alters interaction with TJP1.

Subcellular Location:

Cell junction>Tight junction. Cell membrane>Multi-pass membrane protein.
Note: CLDN4 is required for tight junction localization in the kidney.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Directly interacts with TJP1/ZO-1. Interacts with TJP2/ZO-2 and TJP3/ZO-3 (By similarity). Interacts with EPHA2; phosphorylates CLDN4 and may regulate tight junctions. Interacts with CLDN1. Interacts with CLDN8 (By similarity).

(Microbial infection) Interacts (via both extracellular domains) with Clostridium perfringens enterotoxin CPE; the interaction may disrupt claudin assembly in tight junctions.

Family&Domains:

Belongs to the claudin family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).   (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

References

1). 25-Hydroxycholesterol Exacerbated Experimental Colitis by Inhibiting Tissue Resident Memory ΓδT17 Cells Via Transcription Factor RORγt and Impairing the Mucosal Barrier.

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