FRA2 Antibody - #AF5345

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Product: FRA2 Antibody
Catalog: AF5345
Description: Rabbit polyclonal antibody to FRA2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 35 kDa; 35kD(Calculated).
Uniprot: P15408
RRID: AB_2837830

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
FRA2 Antibody detects endogenous levels of total FRA2.
RRID:
AB_2837830
Cite Format: Affinity Biosciences Cat# AF5345, RRID:AB_2837830.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FLJ23306; Fos L2; FOS like antigen 2; Fos related antigen 2; Fos-related antigen 2; FosL 2; Fosl2; FOSL2_HUMAN; FRA 2; FRA-2;

Immunogens

Immunogen:
Uniprot:

P15408(FOSL2_HUMAN) >>Visit HPA database.

Gene(ID):
FOSL2(2355)
Description:
Controls osteoclast survival and size (By similarity). As a dimer with JUN, activates LIF transcription (By similarity). Activates CEBPB transcription in PGE2-activated osteoblasts.
Sequence:
MYQDYPGNFDTSSRGSSGSPAHAESYSSGGGGQQKFRVDMPGSGSAFIPTINAITTSQDLQWMVQPTVITSMSNPYPRSHPYSPLPGLASVPGHMALPRPGVIKTIGTTVGRRRRDEQLSPEEEEKRRIRRERNKLAAAKCRNRRRELTEKLQAETEELEEEKSGLQKEIAELQKEKEKLEFMLVAHGPVCKISPEERRSPPAPGLQPMRSGGGSVGAVVVKQEPLEEDSPSSSSAGLDKAQRSVIKPISIAGGFYGEEPLHTPIVVTSTPAVTPGTSNLVFTYPSVLEQESPASPSESCSKAHRRSSSSGDQSSDSLNSPTLLAL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P15408 As Substrate

Site PTM Type Enzyme
M1 Acetylation
Y2 Phosphorylation
Y5 Phosphorylation
S16 Phosphorylation
S17 Phosphorylation
S19 Phosphorylation
K35 Ubiquitination
S79 Phosphorylation
S83 Phosphorylation
K104 Acetylation
K104 Methylation
K104 Ubiquitination
S120 Phosphorylation
K151 Ubiquitination
K168 Ubiquitination
S194 Phosphorylation
S200 Phosphorylation
S211 Phosphorylation
S215 Phosphorylation
K222 Acetylation
K222 Sumoylation
S230 Phosphorylation
S232 Phosphorylation
S233 Phosphorylation
S235 Phosphorylation
K240 Acetylation
K240 Ubiquitination
S307 Phosphorylation
S308 Phosphorylation
S309 Phosphorylation
S310 Phosphorylation
S317 Phosphorylation
S320 Phosphorylation
T322 Phosphorylation

Research Backgrounds

Function:

Controls osteoclast survival and size. As a dimer with JUN, activates LIF transcription. Activates CEBPB transcription in PGE2-activated osteoblasts.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Heterodimer.

Family&Domains:

Belongs to the bZIP family. Fos subfamily.

Research Fields

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

References

1). FOSL2 participates in renal fibrosis via SGK1-mediated epithelial-mesenchymal transition of proximal tubular epithelial cells. Journal of Translational Internal Medicine, 2023 (PubMed: 37662889) [IF=4.9]

Application: WB    Species: Mouse    Sample: kidneys

Figure 2 The expression level of FOSL2 is elevated in UUO-injured kidneys. (A) Schematic diagram of UUO and adenovirus infection experiments in mice. (B) Western blotting analyses of protein expression levels of α-SMA, fibronectin, and collagen type I at days 3, 7, and 14. (C) Immunohistochemistry was conducted with anti-FOSL2 antibody to demonstrate FOSL2 protein expression in renal tissues. Magnification: 400×. Scale bar: 50 μm. (D) The mRNA and protein expression levels of FOSL2 were analyzed using qRT-PCR and western blotting, respectively. The values are expressed as mean ± standard deviation. *P < 0.05 versus sham (Day 3) group; ##P < 0.01 versus sham (Day 7) group, ++P < 0.01 versus sham (Day 14) group. UUO: unilateral ureteral obstruction; α-SMA: α-smooth muscle actin; qRT-PCR: quantitative real-time polymerase chain reaction; FOSL2: fos-related antigen 2.

Application: IHC    Species: Mouse    Sample: kidneys

Figure 2 The expression level of FOSL2 is elevated in UUO-injured kidneys. (A) Schematic diagram of UUO and adenovirus infection experiments in mice. (B) Western blotting analyses of protein expression levels of α-SMA, fibronectin, and collagen type I at days 3, 7, and 14. (C) Immunohistochemistry was conducted with anti-FOSL2 antibody to demonstrate FOSL2 protein expression in renal tissues. Magnification: 400×. Scale bar: 50 μm. (D) The mRNA and protein expression levels of FOSL2 were analyzed using qRT-PCR and western blotting, respectively. The values are expressed as mean ± standard deviation. *P < 0.05 versus sham (Day 3) group; ##P < 0.01 versus sham (Day 7) group, ++P < 0.01 versus sham (Day 14) group. UUO: unilateral ureteral obstruction; α-SMA: α-smooth muscle actin; qRT-PCR: quantitative real-time polymerase chain reaction; FOSL2: fos-related antigen 2.

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