Fibronectin Antibody | Affinity Biosciences

Product:	Fibronectin Antibody
Catalog:	AF5335
Description:	Rabbit polyclonal antibody to Fibronectin
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Sheep, Rabbit
Mol.Wt.:	263 kDa, 200 kDa; 272kD(Calculated).
Uniprot:	P02751
RRID:	AB_2837820

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(92%), Bovine(100%), Sheep(92%), Rabbit(100%)

Clonality:

Polyclonal

Specificity:

Fibronectin Antibody detects endogenous levels of total Fibronectin.

RRID:

AB_2837820
Cite Format: Affinity Biosciences Cat# AF5335, RRID:AB_2837820.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CIG; Cold insoluble globulin; Cold-insoluble globulin; DKFZp686F10164; DKFZp686H0342; DKFZp686I1370; DKFZp686O13149; ED B; Fibronectin 1; FINC; FINC_HUMAN; FN; FN1; FNZ; GFND; GFND2; LETS; Migration stimulating factor; MSF; Ugl-Y3;

Immunogens

Immunogen:

Uniprot:

P02751(FINC_HUMAN) >>Visit HPA database.

Gene(ID):

FN1(2335)

Expression:

P02751 FINC_HUMAN:

Expressed in the inner limiting membrane and around blood vessels in the retina (at protein level) (PubMed:29777959). Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine (PubMed:17614963).

Description:

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization

Sequence:

P02751 FINC_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLRGPGPGLLLLAVQCLGTAVPSTGASKSKRQAQQMVQPQSPVAVSQSKPGCYDNGKHYQINQQWERTYLGNALVCTCYGGSRGFNCESKPEAEETCFDKYTGNTYRVGDTYERPKDSMIWDCTCIGAGRGRISCTIANRCHEGGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEWTCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMMVDCTCLGEGSGRITCTSRNRCNDQDTRTSYRIGDTWSKKDNRGNLLQCICTGNGRGEWKCERHTSVQTTSSGSGPFTDVRAAVYQPQPHPQPPPYGHCVTDSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTEGRQDGHLWCSTTSNYEQDQKYSFCTDHTVLVQTRGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNMKWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVMYRIGDQWDKQHDMGHMMRCTCVGNGRGEWTCIAYSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNCTCFGQGRGRWKCDPVDQCQDSETGTFYQIGDSWEKYVHGVRYQCYCYGRGIGEWHCQPLQTYPSSSGPVEVFITETPSQPNSHPIQWNAPQPSHISKYILRWRPKNSVGRWKEATIPGHLNSYTIKGLKPGVVYEGQLISIQQYGHQEVTRFDFTTTSTSTPVTSNTVTGETTPFSPLVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNIPDLLPGRKYIVNVYQISEDGEQSLILSTSQTTAPDAPPDTTVDQVDDTSIVVRWSRPQAPITGYRIVYSPSVEGSSTELNLPETANSVTLSDLQPGVQYNITIYAVEENQESTPVVIQQETTGTPRSDTVPSPRDLQFVEVTDVKVTIMWTPPESAVTGYRVDVIPVNLPGEHGQRLPISRNTFAEVTGLSPGVTYYFKVFAVSHGRESKPLTAQQTTKLDAPTNLQFVNETDSTVLVRWTPPRAQITGYRLTVGLTRRGQPRQYNVGPSVSKYPLRNLQPASEYTVSLVAIKGNQESPKATGVFTTLQPGSSIPPYNTEVTETTIVITWTPAPRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGLTPGVEYVYTIQVLRDGQERDAPIVNKVVTPLSPPTNLHLEANPDTGVLTVSWERSTTPDITGYRITTTPTNGQQGNSLEEVVHADQSSCTFDNLSPGLEYNVSVYTVKDDKESVPISDTIIPEVPQLTDLSFVDITDSSIGLRWTPLNSSTIIGYRITVVAAGEGIPIFEDFVDSSVGYYTVTGLEPGIDYDISVITLINGGESAPTTLTQQTAVPPPTDLRFTNIGPDTMRVTWAPPPSIDLTNFLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYVVSVSSVYEQHESTPLRGRQKTGLDSPTGIDFSDITANSFTVHWIAPRATITGYRIRHHPEHFSGRPREDRVPHSRNSITLTNLTPGTEYVVSIVALNGREESPLLIGQQSTVSDVPRDLEVVAATPTSLLISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISINYRTEIDKPSQMQVTDVQDNSISVKWLPSSSPVTGYRVTTTPKNGPGPTKTKTAGPDQTEMTIEGLQPTVEYVVSVYAQNPSGESQPLVQTAVTNIDRPKGLAFTDVDVDSIKIAWESPQGQVSRYRVTYSSPEDGIHELFPAPDGEEDTAELQGLRPGSEYTVSVVALHDDMESQPLIGTQSTAIPAPTDLKFTQVTPTSLSAQWTPPNVQLTGYRVRVTPKEKTGPMKEINLAPDSSSVVVSGLMVATKYEVSVYALKDTLTSRPAQGVVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAVPANGQTPIQRTIKPDVRSYTITGLQPGTDYKIYLYTLNDNARSSPVVIDASTAIDAPSNLRFLATTPNSLLVSWQPPRARITGYIIKYEKPGSPPREVVPRPRPGVTEATITGLEPGTEYTIYVIALKNNQKSEPLIGRKKTDELPQLVTLPHPNLHGPEILDVPSTVQKTPFVTHPGYDTGNGIQLPGTSGQQPSVGQQMIFEEHGFRRTTPPTTATPIRHRPRPYPPNVGEEIQIGHIPREDVDYHLYPHGPGLNPNASTGQEALSQTTISWAPFQDTSEYIISCHPVGTDEEPLQFRVPGTSTSATLTGLTRGATYNVIVEALKDQQRHKVREEVVTVGNSVNEGLNQPTDDSCFDPYTVSHYAVGDEWERMSESGFKLLCQCLGFGSGHFRCDSSRWCHDNGVNYKIGEKWDRQGENGQMMSCTCLGNGKGEFKCDPHEATCYDDGKTYHVGEQWQKEYLGAICSCTCFGGQRGWRCDNCRRPGGEPSPEGTTGQSYNQYSQRYHQRTNTNVNCPIECFMPLDVQADREDSRE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Bovine

100

Pig

Sheep

Xenopus

Horse

Dog

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P02751 As Substrate

P02751

Site	PTM Type	Source
S41	Phosphorylation	Uniprot
Y101	Phosphorylation	Uniprot
Y106	Phosphorylation	Uniprot
Y112	Phosphorylation	Uniprot
T136	Phosphorylation	Uniprot
T278	O-Glycosylation	Uniprot
T279	O-Glycosylation	Uniprot
S280	O-Glycosylation	Uniprot
S281	O-Glycosylation	Uniprot
S283	O-Glycosylation	Uniprot
T287	O-Glycosylation	Uniprot
Y372	Phosphorylation	Uniprot
N430	N-Glycosylation	Uniprot
K486	Methylation	Uniprot
N528	N-Glycosylation	Uniprot
N542	N-Glycosylation	Uniprot
Y588	Phosphorylation	Uniprot
Y641	Phosphorylation	Uniprot
T715	Phosphorylation	Uniprot
N877	N-Glycosylation	Uniprot
S904	Phosphorylation	Uniprot
S909	Phosphorylation	Uniprot
Y937	Phosphorylation	Uniprot
T960	Phosphorylation	Uniprot
S968	Phosphorylation	Uniprot
T972	Phosphorylation	Uniprot
K987	Acetylation	Uniprot
N1007	N-Glycosylation	Uniprot
Y1042	Phosphorylation	Uniprot
K1050	Ubiquitination	Uniprot
Y1206	Phosphorylation	Uniprot
N1244	N-Glycosylation	Uniprot
T1271	Phosphorylation	Uniprot
T1276	O-Glycosylation	Uniprot
S1565	O-Glycosylation	Uniprot
S1566	O-Glycosylation	Uniprot
S1567	O-Glycosylation	Uniprot
T1570	O-Glycosylation	Uniprot
T1641	O-Glycosylation	Uniprot
T1743	Phosphorylation	Uniprot
T1762	Phosphorylation	Uniprot
T1786	Phosphorylation	Uniprot
S1833	Phosphorylation	Uniprot
K1837	Ubiquitination	Uniprot
T1840	Phosphorylation	Uniprot
T1842	Phosphorylation	Uniprot
T1855	Phosphorylation	Uniprot
T1860	Phosphorylation	Uniprot
Y1879	Phosphorylation	Uniprot
K1880	Acetylation	Uniprot
Y1884	Phosphorylation	Uniprot
S1982	Phosphorylation	Uniprot
T1999	O-Glycosylation	Uniprot
T2060	O-Glycosylation	Uniprot
T2061	O-Glycosylation	Uniprot
T2064	O-Glycosylation	Uniprot
T2065	O-Glycosylation	Uniprot
T2067	O-Glycosylation	Uniprot
N2108	N-Glycosylation	Uniprot
Y2258	Phosphorylation	Uniprot
Y2312	Phosphorylation	Uniprot
S2318	Phosphorylation	Uniprot
S2341	O-Glycosylation	Uniprot
S2341	Phosphorylation	Uniprot
T2345	O-Glycosylation	Uniprot
T2346	O-Glycosylation	Uniprot
S2349	O-Glycosylation	Uniprot
S2349	Phosphorylation	Uniprot
Y2350	Phosphorylation	Uniprot
Y2353	Phosphorylation	Uniprot
S2354	Phosphorylation	Uniprot
T2363	Phosphorylation	Uniprot
S2384	Phosphorylation	Uniprot

Research Backgrounds

P02751_FINC_HUMAN

Function:

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.

Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.

PTMs:

Sulfated.

It is not known whether both or only one of Thr-2155 and Thr-2156 are/is glycosylated.

Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).

Phosphorylated by FAM20C in the extracellular medium.

Proteolytic processing produces the C-terminal NC1 peptide, anastellin.

Some lysine residues are oxidized to allysine by LOXL3, promoting fibronectin activation and matrix formation.

Subcellular Location:

Secreted>Extracellular space>Extracellular matrix.

Tissue Specificity:

Expressed in the inner limiting membrane and around blood vessels in the retina (at protein level). Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.

Subunit Structure:

Mostly heterodimers or multimers of alternatively spliced variants, connected by 2 disulfide bonds near the carboxyl ends; to a lesser extent homodimers. Interacts with FBLN1, AMBP, TNR, LGALS3BP and COL13A1. Interacts with FBLN7 (By similarity). Interacts with COMP. Interacts with TNR; the interaction inhibits cell adhesion and neurite outgrowth (By similarity). Interacts with FST3 and MYOC.

(Microbial infection) Interacts with S.aureus FnbA.

(Microbial infection) Interacts with M.bovis FbpB via the collagen-binding region.

(Microbial infection) Interacts with recombinant S.pneumoniae PavA (rqcH).

(Microbial infection) Interacts with recombinant S.suis FbpS (rqcH) via fibronectin's N-terminal 30 kDa region.

(Microbial infection) Interacts with fibronectin-binding proteins from other Mycobacteria.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > ECM-receptor interaction. (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.

· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Small cell lung cancer. (View pathway)

References

1). Genomic and Transcriptomic Characterization of Natural Killer T Cell Lymphoma. CANCER CELL, 2020 (PubMed: 32183952) [IF=50.3]

Application: IF/ICC Species: human Sample: NK-92 cells

Figure 6. Biological Function of MGA and BRDT. (D) Immunofluorescence images of E-cadherin (green), fibronectin (red), or vimentin (red) in NK-92 cells transfected with MGA shRNAs or scramble. Cells were counterstained with DAPI (blue). (G) Immunofluorescence images of E-cadherin (green), fibronectin (red), or vimentin (red) in NK-92 cells transfected with pCMV6-BRDT (upper panel) or vector control (lower panel). Cells were counterstained with DAPI (blue).

2). Betulinic acid Attenuated Bleomycin-induced Pulmonary Fibrosis by Effectively Intervening Wnt/β-catenin Signaling. PHYTOMEDICINE, 2021 (PubMed: 33341025) [IF=7.9]

3). Huaier polysaccharides suppress triple-negative breast cancer metastasis and epithelial-mesenchymal transition by inducing autophagic degradation of Snail. Cell and Bioscience, 2021 (PubMed: 34481526) [IF=7.5]

Application: WB Species: human Sample: breast cancer cells

Fig. 1| PS-T inhibits invasion, migration and EMT in breast cancer cells in vitro and in vivo. d 4 T-1 and MDA-MB-231 cells are treated with 5 μg/mL PS-T for 24 h. The levels of each EMT marker are quantifed using the NIH ImageJ software. (mean ± SD, *P < 0.05 and **P < 0.01)

Application: WB Species: Human Sample: breast cancer cells

Fig. 1 PS-T inhibits invasion, migration and EMT in breast cancer cells in vitro and in vivo. a The invasiveness of 4 T-1 and MDA-MB-231 cells after treatment with PS-T at 5 μg/mL for 12 h is evaluated using Transwell invasion assays at 24 h (mean ± SD, ***P < 0.001). b Migratory ability of 4 T-1 and MDA-MB-231 cells after treatment with PS-T at different concentrations is evaluated using scratch assays at different time points (mean ± SD, **P < 0.01, ***P < 0.001). c Mice are treated with normal saline (control), 25 μg/g or 100 μg/g PS-T by oral gavage every other day for 21 days (mean ± SD, *P < 0.05, **P < 0.01, scale bars: 100 μm). d 4 T-1 and MDA-MB-231 cells are treated with 5 μg/mL PS-T for 24 h. The levels of each EMT marker are quantified using the NIH ImageJ software. (mean ± SD, *P < 0.05 and **P < 0.01)

4). Bergenin attenuates bleomycin‐induced pulmonary fibrosis in mice via inhibiting TGF‐β1 signaling pathway. PHYTOTHERAPY RESEARCH, 2021 (PubMed: 34375009) [IF=7.2]

5). Synthesis and discovery of a drug candidate for treatment of idiopathic pulmonary fibrosis through inhibition of TGF-β1 pathway. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY, 2018 (PubMed: 30096654) [IF=6.7]

6). CD39+ Fibroblasts Enhance Myofibroblast Activation by Promoting IL-11 Secretion in Hypertrophic Scars. Journal of Investigative Dermatology, 2022 (PubMed: 34537192) [IF=6.5]

7). Primary Human Trabecular Meshwork Model for Pseudoexfoliation. Cells, 2021 (PubMed: 34943956) [IF=6.0]

Application: WB Species: Human Sample:

Figure 3. TGF-β1 induces expression of pro-fibrotic molecules. Relative mRNA expression of (a) α-SMA (b) COL6A2 (c) Fibrillin (d) Fibronectin (e) PAI-1 (f) UPR genes XBP1 and CCT4 (g) Protein expression of pro-fibrotic molecules after TGF-β1 treatment with densitometry analysis using ImageJ (h). (i) Vimentin expression after 10 ng/mL TGF-β1 treatment and densitometry analysis (j). The error bars represent standard deviation

8). Myricetin ameliorates bleomycin-induced pulmonary fibrosis in mice by inhibiting TGF-β signaling via targeting HSP90β. BIOCHEMICAL PHARMACOLOGY, 2020 (PubMed: 32535102) [IF=5.8]

9). Recombinant truncated latency-associated peptide alleviates liver fibrosis in vitro and in vivo via inhibition of TGF-β/Smad pathway. MOLECULAR MEDICINE, 2022 (PubMed: 35842576) [IF=5.7]

Application: WB Species: Rat Sample: HSC-T6 cells

Fig. 4Detection of the effect of LAP and tLAP on liver fibrosis in HSC-T6 cells. A Detection of the effect of LAP and tLAP on cell proliferation by MTT. B Detection of the effect of LAP and tLAP on cell index by RTCA. C Detection of the mRNA expression α-SMA, Collagen I, Collagen IV, and Fibronectin in HSC-T6 cells. D Detection of the expression of α-SMA, Collagen I and Fibronectin in HSC-T6 cells. **P < 0.01 vs control group; ***P < 0.001 vs control group; #P < 0.05 vs TGF-β1 group; ##P < 0.01 vs TGF-β1 group; ###P < 0.001 vs TGF-β1 group; △P < 0.05, TGF-β1 + tLAP group vs TGF-β1 + LAP group; △△P < 0.01, TGF-β1 + tLAP group vs TGF-β1 + LAP group

Application: IF/ICC Species: Rat Sample: HSC-T6 cells

Fig. 5Detection of fibrosis-related proteins expression in HSC-T6 cells by Immunofluorescence. A Immunofluorescence detection of α‑SMA. B Immunofluorescence detection of collagen I. C Immunofluorescence detection of Fibronectin. DAPI (blue, nuclear stain) and antibodies to α‑SMA, Collagen I and Fibronectin (red), immunofluorescence staining (magnification × 200), Scale bars = 40 μm. **P < 0.01 vs control group; ***P < 0.001 vs control group; #P < 0.05 vs TGF-β1 group; ##P < 0.01 vs TGF-β1 group

10). Antifibrotic Mechanism of Cinobufagin in Bleomycin-Induced Pulmonary Fibrosis in Mice. Frontiers in Pharmacology, 2019 (PubMed: 31572194) [IF=5.6]

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Fibronectin Antibody - #AF5335